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Article

Virulence Traits of Inpatient Campylobacter jejuni Isolates, and a Transcriptomic Approach to Identify Potential Genes Maintaining Intracellular Survival

1
Department of Medical Microbiology and Immunology, University of Pécs Medical School, 7624 Pécs, Hungary
2
Department of Biotechnology, Nanophagetherapy Center, Enviroinvest Corporation, 7632 Pécs, Hungary
3
Institute of Plant Biology, Biological Research Center, 6726 Szeged, Hungary
4
Veterinary Medical Research Institute, Hungarian Academy of Sciences, 1581 Budapest, Hungary
*
Author to whom correspondence should be addressed.
Microorganisms 2020, 8(4), 531; https://doi.org/10.3390/microorganisms8040531
Received: 5 March 2020 / Revised: 1 April 2020 / Accepted: 1 April 2020 / Published: 7 April 2020
(This article belongs to the Special Issue Foodborne Pathogen Campylobacter)
There are still major gaps in our understanding of the bacterial factors that influence the outcomes of human Campylobacter jejuni infection. The aim of this study was to compare the virulence-associated features of 192 human C. jejuni strains isolated from hospitalized patients with diarrhoea (150/192, 78.1%), bloody diarrhoea (23/192, 11.9%), gastroenteritis (3/192, 1.6%), ulcerative colitis (3/192, 1.5%), and stomach ache (2/192, 1.0%). Traits were analysed with genotypic and phenotypic methods, including PCR and extracellular matrix protein (ECMP) binding, adhesion, and invasion capacities. Results were studied alongside patient symptoms, but no distinct links with them could be determined. Since the capacity of C. jejuni to invade host epithelial cells is one of its most enigmatic attributes, a high throughput transcriptomic analysis was performed in the third hour of internalization with a C. jejuni strain originally isolated from bloody diarrhoea. Characteristic groups of genes were significantly upregulated, outlining a survival strategy of internalized C. jejuni comprising genes related (1) to oxidative stress; (2) to a protective sheath formed by the capsule, LOS, N-, and O- glycosylation systems; (3) to dynamic metabolic activity supported by different translocases and the membrane-integrated component of the flagellar apparatus; and (4) to hitherto unknown genes. View Full-Text
Keywords: Campylobacter jejuni; clinical isolates; genomic approach; virulence potential; intracellular survival; transcriptomic analysis Campylobacter jejuni; clinical isolates; genomic approach; virulence potential; intracellular survival; transcriptomic analysis
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MDPI and ACS Style

Kovács, J.K.; Cox, A.; Schweitzer, B.; Maróti, G.; Kovács, T.; Fenyvesi, H.; Emődy, L.; Schneider, G. Virulence Traits of Inpatient Campylobacter jejuni Isolates, and a Transcriptomic Approach to Identify Potential Genes Maintaining Intracellular Survival. Microorganisms 2020, 8, 531. https://doi.org/10.3390/microorganisms8040531

AMA Style

Kovács JK, Cox A, Schweitzer B, Maróti G, Kovács T, Fenyvesi H, Emődy L, Schneider G. Virulence Traits of Inpatient Campylobacter jejuni Isolates, and a Transcriptomic Approach to Identify Potential Genes Maintaining Intracellular Survival. Microorganisms. 2020; 8(4):531. https://doi.org/10.3390/microorganisms8040531

Chicago/Turabian Style

Kovács, Judit K., Alysia Cox, Bettina Schweitzer, Gergely Maróti, Tamás Kovács, Hajnalka Fenyvesi, Levente Emődy, and György Schneider. 2020. "Virulence Traits of Inpatient Campylobacter jejuni Isolates, and a Transcriptomic Approach to Identify Potential Genes Maintaining Intracellular Survival" Microorganisms 8, no. 4: 531. https://doi.org/10.3390/microorganisms8040531

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