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Review

Spatio-Temporal Distribution of Acinetobacter baumannii in Germany—A Comprehensive Systematic Review of Studies on Resistance Development in Humans (2000–2018)

by
Gamal Wareth
1,2,*,
Christian Brandt
3,
Lisa D. Sprague
1,
Heinrich Neubauer
1 and
Mathias W. Pletz
3,4
1
Friedrich-Loeffler-Institut, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743 Jena, Germany
2
Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh 13736, Egypt
3
Institute for Infectious Diseases and Infection Control, Jena University Hospital, Am Klinikum 1, 07747 Jena, Germany
4
Research Campus Infectognostics, Philosophenweg 7, 07743 Jena, Germany
*
Author to whom correspondence should be addressed.
Microorganisms 2020, 8(3), 375; https://doi.org/10.3390/microorganisms8030375
Submission received: 24 January 2020 / Revised: 3 March 2020 / Accepted: 3 March 2020 / Published: 6 March 2020
(This article belongs to the Section Medical Microbiology)
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Abstract

:
Acinetobacter (A.) baumannii has gained global notoriety as a significant nosocomial pathogen because it is frequently associated with multi-drug resistance and hospital-based outbreaks. There is a substantial difference in the incidence of A. baumannii infections between different countries and within Germany. However, its continuous spread within Germany is a matter of concern. A systematic literature search and analysis of the literature published between 2000 and 2018 on A. baumannii in humans was performed. Forty-four studies out of 216 articles met the criteria for inclusion, and were selected and reviewed. The number of published articles is increasing over time gradually. Case reports and outbreak investigations are representing the main body of publications. North Rhine-Westphalia, Hesse and Baden-Wuerttemberg were states with frequent reports. Hospitals in Cologne and Frankfurt were often mentioned as specialized institutions. Multiresistant strains carrying diverse resistance genes were isolated in 13 of the 16 German states. The oxacillinase blaOXA-23-like, intrinsic blaOXA-51-like, blaOXA-58 variant, blaNDM-1, blaGES-11, blaCTX-M and blaTEM are the most predominant resistance traits found in German A. baumannii isolates. Five clonal lineages IC-2, IC-7, IC-1, IC-4 and IC-6 and six sequence types ST22, ST53, ST195, ST218, ST944/ST78 and ST348/ST2 have been reported. Due to multidrug resistance, colistin, tigecycline, aminoglycosides, fosfomycin, ceftazidime/avibactam and ceftolozan/tazobactam were often reported to be the only effective antibiotics left to treat quadruple multi-resistant Gram-negative (4MRGN) A. baumannii. Dissemination and infection rates of A. baumannii are on the rise nationwide. Hence, several aspects of resistance development and pathogenesis are not fully understood yet. Increased awareness, extensive study of mechanisms of resistance and development of alternative strategies for treatment are required. One-Health genomic surveillance is needed to understand the dynamics of spread, to identify the main reservoirs and routes of transmission and to develop targeted intervention strategies.

1. Introduction

Acinetobacter (A.) baumannii is a ubiquitous, Gram-negative, non-motile bacterium associated with hospital-acquired infection globally. It has been classified among the most dangerous multiple drug-resistant (MDR) pathogens worldwide. It has been listed in the “priority 1: critical” pathogens list of the World Health Organization (WHO). The pathogen was found in humans, animals, foods and the environment. A. baumannii is associated with severe infection, high mortality rates and massive economic loss [1]. Since the late 20th century, this bacterium emerged as a major cause of healthcare-associated infection in critically ill patients causing bloodstream infections, ventilator-associated pneumonia, meningitis, endocarditis, wound infection, urinary tract infections, skin and soft tissues infections [2]. Mortality rates can reach 35% in ventilator-associated pneumonia and bloodstream infections [3]. Members of the genus Acinetobacter are usually environmental bacteria. For unclear reasons, A. baumannii emerged as an opportunistic nosocomial pathogen. It has established niches for survival in hospitals as well as in primary animal production systems and the environment [4]. The main vital factors contributing to the extravagant dissemination of A. baumannii in hospitals are a large variety of potential sources of entry, environmental resilience and its ability to develop resistance to the majority of antibiotics, particularly the ability to acquire resistance to carbapenems [1,5]. A. baumannii becomes resistant via reducing membrane permeability, increasing efflux pump activity and production of a wide variety of β-lactamases. It is also able to acquire resistance via mutations and through horizontal gene transfer [6]. A. baumannii exhibits high genetic plasticity, which allows the accumulation of the acquired resistance determinants [7]. Resistance in this pathogen is frequently associated with mobile genetic elements (MGEs) that are transferable between bacteria, thus enabling the rapid dissemination of resistance genes between bacteria of different species and creating a reservoir of antimicrobial-resistance (AMR) genes [7].
For several years, the prevalence of infection with MDR A. baumannii has been increasing dramatically in the European countries that is, from 15.4% in 2004 to 48.5% in 2014 [8]. In the late 20th century, A. baumannii has emerged as a significant nosocomial pathogen in Germany [9,10,11]. Numerous nosocomial outbreaks were reported among inpatients and preterm infants with a considerable mortality rate of up to 24% after bacteremia in intensive care units at a large teaching hospital of Cologne [12]. Since 2000, the number of reported cases and hospital outbreaks was increasing continuously in most German states [13,14,15,16,17]. Isolates with resistance to all β-lactams, including carbapenems [18] and last resort antimicrobial agents such as colistin and tigecycline [19], were reported nationwide and have become a significant cause of worry of the healthcare system. Several studies on A. baumannii have been published from different German states but a systematic review on its spatial and temporal distribution is not yet available. Thus, the current report aims at providing a comprehensive, evidence-based assessment of the literature published from 2000-2018 on A. baumannii in Germany.

2. Materials and Methods

2.1. Data Sources and Search Strategy

A comprehensive search in PubMed, Web of Science, Scopus and the catalogue of the German National Library was performed for articles that include the search terms “Acinetobacter baumannii” and “Germany” in their title and abstract. All national and international studies discussing A. baumannii in Germany published in both English and German language were collected. References cited within these papers were checked to find additional relevant information.

2.2. Selection Criteria and Data Extraction

Studies discussing isolation, epidemiology and antimicrobial resistance of A. baumannii from 2000 through 2018 were included. Review articles, congress abstracts, case reports, letters, commentaries and editorials were included. Abstract analysis for each publication was done and, if selected, the full text was carefully analyzed. The following keywords were used—Acinetobacter baumannii + Germany + human + antimicrobial resistance. Articles describing other Acinetobacter spp., A. baumannii in non-human sources, novel diagnostics, development of PCR and studies published before the year 2000 were excluded. For each study, following information was extracted from the full text—first author, year of publication, sampling date, location of sampling (city and state), study population, type and source of samples, number of strains and cases, type of study, resistance profiles of the recovered strains including phenotypic testing and detection of resistance genes. If available, the sequence and clonal type of strains were included.

2.3. Data Acquisition and Analysis

Overall, a total of 216 potentially relevant articles [PubMed (n = 114), Scopus (n = 69) and Web of Science (n = 33)] were identified. Sixty-one articles were selected for screening and forty-four studies were included in the review (Figure 1: PRISMA diagram). The information was categorized starting with the latest one.

2.4. Relative Research Interest

The number of publications that are available on PubMed for each year was extracted and the relative research interest was calculated [20]. It is defined as the quotient of specific articles divided by all articles in this year to consider the overall publication growth per year.

3. Results

3.1. Data Analysis

Case reports represented the majority of the published articles (n = 14), followed by outbreak reports (n = 7) and evaluation of antibiotic activity (n = 6). The remaining studies were surveillance studies, routine microbiological examination and descriptive data analysis. Thirty-seven articles were published in English and seven in German [15,16,17,18,19,20,21]. The sampling date was not reported in 15 studies, while the geographical location of samples was ignored in three studies. Lost information was categorized as “non-determined” and referred to as ‘ND’ in the tables. A. baumannii was most often isolated from the bloodstream, wound infections and respiratory and urinary tract infections as well as from skin and rectal swabs. In outbreak investigations it was also isolated from the patient’s environment, medical devices, patient’s associated belongings, room equipment, infusion pumps and oxygen masks [20,22]. In the catalogue of the German National Library, 148 doctoral theses containing the word Acinetobacter in their titles were identified. Twenty-one of these were published between 2000–2018. Only nine dissertations with emphasis on isolation, molecular and functional typing, distribution and genetic composition of AMR determinants were found (data are not shown). Three of these were done in Bonn, three in Cologne and one each in Berlin, Freiburg and Frankfurt am Main representing the main centres of A. baumannii research.

3.2. Temporal Distribution of the Analyzed Studies

The emergence of MDR in A. baumannii has been steadily increasing over time and the number of published articles reflects this fact. The relative research interest has been growing after 2013. The year 2010 was super crucial for the research on A. baumannii in Germany. PubMed analysis using the search term ‘A. baumannii in Germany’ showed a gradual increase in the number of published studies over time with the highest number of annual articles in 2018 (Figure 2). Finally, 44 articles were analyzed. Approximately two thirds of studies (n = 32) were published in or after 2010, of them 13 articles have been published in the years 2016–2018 (Table 1).

3.3. Spatial Distribution of the Analyzed Studies Based on Geographical Location of Sampling

Most of the published articles are regional studies. Only six studies included samples throughout Germany but without the exact geographical origin of samples. North Rhine-Westphalia, Hesse and Baden-Wuerttemberg contributed eleven, eight and five studies, respectively, followed by Bavaria (four studies), Saxony (three studies) and Berlin and Schleswig-Holstein (two studies each). From Thuringia, Hamburg, Lower Saxony, Saxony-Anhalt, Rhineland-Palatinate and Mecklenburg-Vorpommern, only one study was accessible. Brandenburg, Bremen and Saarland are not present at all. Cologne, the largest city of North Rhine-Westphalia, was the most represented city and was mentioned in six studies. Frankfurt am Main, the largest city of Hesse, was mentioned in five studies. Both cities are centers of A. baumannii research. Munich, the largest city of Bavaria, contributed three studies. Leipzig, one of the biggest cities of Saxony, Tuebingen and Freiburg, two cities in Baden-Württemberg, were study areas for two studies each (Table 1).

3.4. Official Data Concerning the Infection and Epidemiology of Acinetobacter in Germany

Within the framework of the Infection Protection Act in Germany, the Robert Koch Institute (RKI) is responsible for collecting data on notifiable diseases. The number of nosocomial outbreaks and the number of cases in each outbreak, as well as the number of deaths were recorded for 2010 onwards [59]. As shown in Table 2, no information has been documented in 2010 and 2011 and for the years 2012, 2013 and 2016 the highest numbers of nosocomial outbreaks were reported. The annual statistics on reportable diseases of Germany reported 794 and 784 Acinetobacter infections or colonization in 2017 and 2018, with an incidence of 1.0 and 0.9, respectively. North Rhine-Westphalia reported the highest numbers of Acinetobacter infections or colonizations with 405 notifications, followed by Bavaria (n = 205), Hesse (n = 200) and Berlin (n = 196) [60]. In contrast, the lowest incidences were reported for the federal states of Bremen (n = 6) and Saarland (n = 5). It has to be taken into consideration that the numbers mentioned in the RKI statistics do not represent the total number of infections in Germany because only the carbapenem-non-susceptible isolates are reported to the RKI and species of Acinetobacter are not differentiated.

3.5. Identification of A. baumannii and Antibiotic Susceptibility Testing (AST)

Identification of A. baumannii has been done mostly by the VITEK-2 system, MALDI-TOF [18,26] and detection of the intrinsic blaOXA-51-like gene [30]. Phenotypic antibiotic sensitivity testing (AST) was performed with the VITEK-2 platform, E-test [28], disk diffusion method [24], antibiotic gradient test and micro-broth dilution using Micronaut-S system [22]. The National Committee for Clinical Laboratory Standards (NCCLS) broth microdilution method and the respective breakpoints were used until 2006 [52]. The broth dilution test was performed following the guidelines of the German Institute for Standardization (DIN) in 2010 [46] and 2013 [39]. The phenotypic detection of resistance in most of the published studies was performed following the Clinical and Laboratory Standards Institute (CLSI) recommendations and the European Committee on Antimicrobial Susceptibility Testing (EUCAST). No breakpoint for tigecycline is approved by EUCAST [49,50]. Thus, the breakpoint of EUCAST for Enterobacteriaceae has been used [16].

3.6. Resistance Development of A. baumannii

At the beginning of the 21st century, A. baumannii attracted the attention of researchers due to its rapid spread and its resistance to most of the antibiotics used. In a surveillance study performed from 2001 to 2008 in 53 German intensive care units (ICUs), the resistance to imipenem increased from 1.1% in 2001 to 4.5% in 2008 [61]. From 2002 to 2006, the rate of isolation of A. baumannii increased in university hospitals of six German federal states (Baden-Wuerttemberg, Hesse, Lower Saxony, Saxony, Schleswig–Holstein and Thuringia) from 2.1% in 2002 to 7.9% in 2006 [46] and the proportion of MDR of A. baumannii isolates raised among inpatients [46]. XDR strains resistant to all available antibiotics except colistin have been isolated from wound, skin and respiratory tract samples in an outbreak in 2003 [14]. To overcome the resistance developed against broad-spectrum antibiotics tetracycline, tigecycline a new class of antibiotics derived from tetracycline has been implemented in 2006. Between 2004 and 2007, tigecycline had excellent activity against A. baumannii and other Gram-negative nosocomial bacteria [49,50,51]. Until 2005 carbapenems remained the gold standard of therapy for severe A. baumannii infections and resistance to carbapenems remained low (0.3%) [54]. In 2006, the first outbreak of carbapenem-resistant A. baumannii (CRAb), carrying the blaOXA-23-like gene, was reported in Germany. The strain was resistant to imipenem, meropenem, penicillins, cephalosporins, ciprofloxacin, gentamicin, tobramycin and tigecycline and was related to the pan-European A. baumannii clone II [16]. Since then, carbapenemase-producing strains have been recovered from several outbreaks every year [26,27,30,33,62]. According to the National Reference Laboratory for Gram-negative nosocomial pathogens at the Ruhr-University Bochum, CRAb was found in 96.3% of strains and blaOXA-23 was the most frequent carbapenemase in A. baumannii (81.1%) in 2011 [41]. This high resistance rates can be explained by the practice that only non-susceptible strains are sent to the National Reference Laboratory for clarification. It is worth to mention that, the number of cases of carbapenem insensitive Acinetobacter infection or colonization was 794 in 2017 and 784 in 2018 [60]. In contrast, the ARS data demonstrate that only 4% of A. baumannii ABC complex strains were not carbapenem sensitive in 2015 to 2018 (https://ars.rki.de/Content/Database/ResistanceOverview.aspx).
Susceptibility to tigecycline and colistin was reported in four and nineteen studies, respectively (Table 3). Susceptibility to colistin and tigecycline remained unaffected until December 2013. At this time, the first reported pan drug-resistant (PDR)-CRAb strain was recovered from a patient at Goethe University hospital Frankfurt after a previous hospitalization in Greece. This strain was also resistant to colistin [19]. Until 2018 colistin-resistant strains were detected three times in Germany. One was isolated from a human in 2013 in Frankfurt [19], one from a dog in Giessen in 2011 [63] and one from wastewater in 2017 [64]. It has to be taken in consideration that most of the earlier colistin testing was performed by gradient tests for example, disc diffusion, E-test or with VITEK. EUCAST has revised the recommendation for colistin AST and microdilution technique is considered the only reliable method [65]. Tigecycline resistant strains were reported five times: once in 2016 in Leverkusen [30], once in 2014 from Frankfurt [19], two times in 2010 from the university hospital Frankfurt [47] and from the university hospital Rostock [15] and once in 2009 with undetermined location [16]. Colistin, tigecycline, aminoglycosides, fosfomycin, ceftazidime/avibactam and ceftolozan/tazobactam are often used antibiotics to treat 4MRGN A. baumannii infection in Germany [66]. Information regarding the resistance patterns of A. baumannii are shown in Table 3.

3.7. Resistance Genes in A. baumannii

Twenty-eight of the selected studies described AMR genes. As shown in Table 3, the metallo-ß-lactamase was first described in studies published in 2004, 2005 and 2006 [14,52,53]. The oxacillinase blaOXA-23-like, the intrinsic blaOXA-51-like, the New Delhi Metallo-ß-lactamases (blaNDM) and the blaOXA-58-like variant are the most dominant resistance genes that have been described in A. baumannii from Germany (Table 3). The gene blaOXA-23-like, the intrinsic blaOXA-51-like and the blaOXA-58-like variants were found in a CRAb strain for the first time in an outbreak, which occurred at a German university medical center in 2006. However, the location of the sampling was ignored [16]. Those three genes have been reported later on in sixteen, elven and seven studies, respectively. In 2011, the National Reference Laboratory for Gram-negative nosocomial pathogens at Ruhr-University Bochum found that the blaOXA-23 was the most frequent carbapenemase in A. baumannii [41]. The first isolation of a blaNDM-1 strain was reported in 2007 in Frankfurt [47] and then in ten other studies. Additionally, Guiana extended-spectrum β-lactamases (GES-11) [41], Cefotaxime Hydrolyzing Capabilities (CTX-M) β-lactamases [28], Temoneira (TEM) and Plasmid-Mediated Quinolone Resistance (PMQR) [35], German imipenemase (GIM-1), VIP-2 and Verona integron-encoded-metallo-β-lactamase (VIM-2) [32] and blaADC-25-like [18] have been described in A. baumannii (Table 3). Figure 3 represents the chronological emergence of resistance to carbapenems, colistin, tigecycline and quinolones antibiotics as well as the resistance genes according to time of sampling or time of reporting if the time of sampling is not available.

3.8. Clonality and Sequence Typing

Cluster analysis based on Pulsed-field Gel Electrophoresis (PFGE) and rep-PCR typing identified five clonal lineages of A. baumannii in Germany, IC-2, IC-7, IC-1, IC-4 and IC-6. The international clone IC-2 is the most common clonal lineage widespread in Germany and was present in seven studies. Followed by IC-7 and IC-1, each was reported in two studies, while IC-4 and IC-6, each was identified only in one study. IC-2 was detected in five studies alone [16,19,27,33,42] and in combination with IC-6 once [28] and with IC-1, IC-7 and IC-4 in another study during the investigation of strains collected from different laboratories in the years 2005, 2007 and 2009 [39]. Only six sequence types of A. baumannii have been reported in the reviewed articles. Multilocus sequence typing (MLST) revealed ST22 and ST53 types among 13 strains recovered from an outbreak of seven patients in Rostock [15]. ST195 and ST218 were identified in an outbreak involving ten patients in Leverkusen [30]. ST944/ST78 and ST348/ST2 were determined in three isolates recovered from two patients hospitalized in Bavaria and Hesse [28].

3.9. The Risk Factors Associated with A. baumannii Infections

Investigation and outbreak studies, as well as case reports analyzed in this review, have reported multiple risk factors for the emergence and acquisition of A. baumannii infections. Firstly, travel, medical tourism or contact with health care systems abroad, as well as cross border transfer of patients from highly endemic countries into Germany, lead to the import and emerging presence of A. baumannii in the German hospitals. Between June and December 2015, 143 refugees have been admitted to the University hospital Frankfurt, Hesse. 60.8% were positive for MDR Gram-negative bacteria and CRAb strains harboring blaOXA-23 and OXA-24 genes were reported in 1.4% of clinical samples examined [27]. blaOXA-23 and blaOXA-48, the most frequently reported resistance genes in Germany, were detected with high prevalence among Libyan war casualties admitted to the Northwest Medical Center (NMC) in Frankfurt/Main, Hesse between August 2016 and January 2017 [24]. MDR A. baumannii was recovered from patients in German hospitals after previous hospitalization in Russia [28,34], Thailand [30], Serbia [42,47], Poland [43], China, the United Arabic Emirates and Croatia [37], Greece and Italy [53] and other Mediterranean countries [52]. A patient was infected in Cameroon [14]. First reports on PDR (pan-drug resistant) A. baumannii appeared in 2001 and an outbreak was reported in Spain in 2002 [67]. Subsequently, the first isolation of a PDR strain in Germany belonging to the IC-2 cluster was done from the skin and rectal swab samples of a patient previously hospitalized in Greece [19]. In contrast, a CRAb strain harboring blaOXA-23 was isolated from a hip joint infection in a patient with no history of traveling abroad at the University hospital Bonn [33]. Between 2005 and 2009, 140 CRAb strains were isolated in 15 different medical centers in Germany, only 32% of these strains belonged to one of the known international clonal lineages [39].
Secondly, during a (long-term) ICU stay, A. baumannii can be acquired via mechanical ventilation. An epidemic strain of A. baumannii was transferred from the index patient to other nine patients at the same hospital in Leverkusen, North Rhine-Westphalia within 16 days [30]. Cross-transmission via the hands of health care workers from colonized or infected patients has been described in an epidemic setting in a German university medical center in Berlin [16]. In one year, 44 individual patients acquired infection in a hospital in Cologne. A. baumannii was recovered from the washbasin and fixation bath [18], from medical devices, infusion pumps and oxygen masks [15,16]. An identical macrorestriction pattern (PFGE-type) was detected in isolates of two different hospitals in 2008 in North Rhine-Westphalia confirming that inter-hospital transmission is possible [26]. Moreover, a prolonged hospital stay is frequently associated with exposure to antibiotics [68]. A. baumannii can rapidly acquire resistance by mutation during antimicrobial therapy [44]. Selective pressure due to preceding antimicrobial treatment was a significant factor in acquiring carbapenem-resistance in a study from Hamburg-Eppendorf, Hamburg [21].
Finally, the environmental resilience of A. baumannii and environmental contamination facilitates the spread and dissemination of infection from colonized or infected patients. Examination of environmental samples during an outbreak revealed relevant dissemination of A. baumannii [18]. A. baumannii was isolated from the patients’ environment, patients’ belongings and room equipment [15,16]. A. baumannii can survive in the environment for a prolonged time and it can colonize non-human hosts for example, pet animals with ease [69]. The occurrence of genotypically related clones I–III in animals and humans in Germany raises the concern about the possibility of a spillover of the organisms from humans to animals or vice versa [63]. Strains harboring resistance genes coding for clinically relevant antibiotics can enter the clinical setting via food and community routes [70,71]. Thus, humans can be colonized by A. baumannii through the food chain or from other environmental sources for example, wastewater, hatchery system and dust.

4. Discussion

Acinetobacter baumannii is one of the most threatening emerging environmental pathogens, causing particularly nosocomial infections in humans in Germany. The number of published studies concerning A. baumannii is dramatically increasing worldwide and also in Germany. This systematic review investigated the spatial and temporal distribution of A. baumannii in humans in Germany based on published data from 2000 to 2018. The pathogen was extensively studied in the last few years. This increase of interest reflects the increasing clinical impact and its ability to acquire resistance against the majority of antibiotics. A. baumannii MDR strains that is, strains with resistance to all β-lactams including carbapenems and production of β-lactamases of several types, were reported nationwide. A prominent number of published articles originates from North Rhine-Westphalia. This state is located in the West of Germany and has the highest population of German states. A considerable number of reports also describe the situation of Hesse, which is a central German state. The cities Cologne and Frankfurt-am-Main are centres of A. baumannii research and are home to tertiary care clinics and hospitals which treat a relatively high numbers of patients in ICUs. These hospitals also run large highly specialized treatment units that received patients from abroad [18,24]. The obtained results are in harmony with the official data of the Robert Koch Institute (RKI) considering numbers of infection or colonization of carbapenem non-susceptible Acinetobacter spp. and incidence [60]. The official data published by RKI were included to avoid loss of information, aware the shortcoming of these data that is, no differentiation between the different species was made and that numbers do not present the total number of infections in Germany, as only carbapenem non-susceptible strains have to be notified. Information published by the ARS project cover data for the whole A. baumannii complex group.
The identification of A. baumannii was mostly based on the VITEK-2 system, MALDI-TOF and PCR. The failure of the semi-automated VITEK-2 system to correctly identify A. baumannii and predict carbapenem susceptibility is well known [44]. MALDI-TOF log score values >2000 were accepted in some laboratories for species identification [72]. However, evaluation of species-specific score cut-off values of clinical A. baumannii revealed that a score value > 2.3 is the valid score value for species identification [73]. Species misidentification and false AST may negatively influence clinical outcome. A. baumannii is part of the ACB complex and it is difficult to distinguish A. baumannii from other species phenotypically. A proper, accurate and reliable molecular identification of A. baumannii to species level is needed and a typing method with high discriminatory power is crucial [18,44] for diagnosis, epidemiological trace back and vaccine production. Rates of colonization and infection with triple (3MRGN) and quadruple (4MRGN) A. baumannii are on the rise in Germany [17,29,36]. However, this type of classification is specific for Germany only. Carbapenems are the gold standard to date for the treatment of severe infections with MDR strains. However, the appearance of CRAb poses new challenges for treatment strategies [66]. Nevertheless, colistin and tigecycline are therapeutic options and showed efficacy in the treatment of MDR strains [15,25,37,51]. The appearance of colistin and tigecycline resistant [47] and colistin-resistant strains carrying the mcr-1 gene in non-human sources [64] will result in limited effectiveness in the near future. Several methods have been used to detect colistin and tigecycline resistant strains. The lack of EUCAST and CLSI breakpoints for tigecycline hampers the detection of tigecycline resistance and is a big challenge for clinicians [74]. Accordingly, the breakpoint for Enterobacteriaceae was recommended and used in different studies in the past [16]. However, caution is needed during interpretation. A. baumannii is intrinsically resistant to fosfomycin [75]. Only six articles were found discussing resistance to fosfomycin. The first fosfomycin resistant A. baumannii isolate was reported from an outbreak at the university hospital Tuebingen in 2004 [14] and then MDR strains resistant to fosfomycin were reported again in 2010, 2011, 2014 and 2017 [19,25,43,44]. According to the Surveillance of Antibiotic Use and Resistance in Intensive Care Units (SARI-ICUs) project, that was initiated in Germany in 2000, the resistance rate of MRGN pathogens increased markedly from 2001 to 2015 [76]. The increase in A. baumannii isolates with resistance to imipenem has been reported from 2005 onwards with a pronounced trend. The resistance rate has more than doubled over recent years and reach to 43% in 2015 [76]. The antibiotic resistance surveillance (ARS) project revealed that, the level of resistance in A. baumannii isolates obtained from patients in ICUs is about three times that higher than of isolates obtained from patients in normal and outpatient settings. 7.6% of A. baumannii strains were carbapenem resistant in 2014 and 4% in 2017 and 2018. However, it has to be kept in mind that this information is not restricted to A. baumannii but include all of the A. baumannii complex group (https://ars.rki.de/Content/Database/ResistanceOverview.aspx).
The blaOXA-23, blaOXA-51-like and blaOXA-58-like, as well as blaNDM-1 were the resistance genes most often found in A. baumannii in the last ten years followed by blaGES-11, blaCTX-M and blaVIM. Since the first description of the blaOXA-23 in a clinical isolate of A. baumannii in Scotland in 1995 [77], A. baumannii harboring the blaOXA-23 gene spread to hospitals worldwide and were also detected in companion animals, livestock, environment and ectoparasites [78]. It has become the most frequent carbapenemase gene among A. baumannii in most countries. Transfer of resistance genes from bacteria of other genera is a predominant mechanism of acquiring resistance to carbapenems in A. baumannii. The blaNDM-1 was identified mainly in E. coli and K. pneumoniae and to a lower extent in Pseudomonas and Acinetobacter. The first occurrence of A. baumannii carrying blaNDM-1 in Germany dates bake to the year 2007 [47,48]. Hence, current PCR protocols only identify resistance-coding genes but provide no in-depth information regarding the functions and mechanisms of resistance development or actual clinical resistance. Detection and characterization of AMR genes are gradually moving from PCR to high throughput identification via sequencing and in-silico detection utilizing several databases now. However, the use of next-generation sequencing (NGS) to investigate AMR in A. baumannii is still rare and has just begun to start in Germany.
This discriminatory power enables transmission-chain analysis, optimizes surveillance and promotes suitable containment measures. It also offers the traits to identify transmission dynamics [79]. Molecular characterization of AMR in A. baumannii often has been performed by using conventional methods and very few studies utilizing the WGS technology have been performed [18,31,35]. For outbreak investigation, PFGE has been considered the gold standard for a long time but it has been replaced by NGS technology due to its higher discriminatory properties. Most of the WGS based studies on A. baumannii were performed in outbreak settings and there is little data on WGS in the context of surveillance.
International travel of colonized patients, especially from countries with high prevalence for example, Mediterranean and Asian countries has resulted in and still resulting in the introduction and later spread of MDR A. baumannii to Europe and subsequently into Germany [53]. Following a fatal outbreak of six patients with A. baumannii infection-related death at a tertiary care facility in the northwest United States, comparative genomics of strains showed a close relation to strains isolated from Germany in 2003 [80]. Isolates from Germany were found to share the same rep-PCR pattern with isolates from Turkey and the USA [81] and identical to those from isolates from the UK [82]. Thus, cross border transmission of MDR A. baumannii or its resistance genes have to be considered and the colonization status of patients treated abroad should be handled with care to avoid the spread of this pathogen into countries with low incidence rates [53]. WGS can be the tool of choice to assist public health officer and clinicians to contain spread and to initiate well timed start of treatment with the best medication.

5. Conclusions

In conclusion, the published knowledge on the existence and general distribution of A. baumannii in the German population is gradually increasing over time but is mainly based on regional studies, outbreak investigations and case reports. Only few countrywide studies have been done yet. Several aspects of resistance development remain cryptic. Large cities with tertiary care hospitals and vital tourism are hot spots for research and publication activity on A. baumannii. MDR strains harboring diverse resistance genes were found in most of the federal states. Five clonal lineages and six sequence types of A. baumannii have been identified within the last 18 years in Germany. A. baumannii is part of the ACB complex and it is difficult to distinguish it from other species. Due to clinical needs, its differentiation from other members of the ACB complex species, particularly the closely related A. pittii and A. nosocomialis is required. Accurate and safe diagnostics for species identification as well as the definition of breakpoints for all antibiotics used needs to be established. Several risk factors contributing to the spread and dissemination of infection have been identified for example, travelling, medical tourisms, long-term stay at ICUs and the environmental contamination. Dissemination and infection rates of MDR (particularly those known in Germany as 3MRGN-Ab and 4MRGN-Ab) are on the rise nationwide and need to be considered a threat to public health.
Due to the nature of the data reported to RKI they cannot differentiate between the different species causing infection or colonization. The ARS project shaves this limitation as it collects data on the A. baumannii complex following a holistic approach. The reported data published by the National Reference Laboratory and the RKI do not sum up the total number of infections or colonizations in Germany because only carbapenem non-susceptible Acinetobacter strains need to be reported to the NRL and RKI. The nationwide One-Health genomic surveillance of A. baumannii using NGS technology to understand the dynamics of resistance and mobile genetic elements (MGEs) has to be in the center of public efforts, now the NGS is afford and available. Especially pets, livestock, food and the environment are neglected reservoirs and isolates from theses niches need to be investigated to assess their impact on human health and economy. Thus, identification of novel and alternative strategies for treatment, collaboration between the veterinary and human health sectors and the increase of awareness in the public and in the risk group are fields identified to be improved.

Author Contributions

G.W. performed the online database query. G.W. and L.D.S. read abstracts and selected studies for full text review. C.B. carried out the relative research interest and associated figure. G.W. wrote the manuscript. H.N. and M.W.P. supervised the review. All authors have read and agreed to the published version of the manuscript.

Funding

This study was supported by internal funding of the Friedrich-Loeffler-Institute. C.B. and M.W.P. were co-funded by grants from the Federal Ministry of Education and Research (Germany), Grant Numbers 13GW0096D and 01KI1501.

Conflicts of Interest

The authors declare no conflict of interest.

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Figure 1. Systematic review flowchart: Acinetobacter baumannii in Germany, the flow diagram indicates the inclusion and exclusion of publications at each stage of the systematic review process.
Figure 1. Systematic review flowchart: Acinetobacter baumannii in Germany, the flow diagram indicates the inclusion and exclusion of publications at each stage of the systematic review process.
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Microorganisms 08 00375 g002 550
Figure 2. PubMed analysis showing the relative research interest for A. baumannii in Germany from 2000–2018. Publications analyzed in this work a marked orange.
Figure 2. PubMed analysis showing the relative research interest for A. baumannii in Germany from 2000–2018. Publications analyzed in this work a marked orange.
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Microorganisms 08 00375 g003 550
Figure 3. Chronological emergence of ß-lactam resistance in A. baumannii in Germany.
Figure 3. Chronological emergence of ß-lactam resistance in A. baumannii in Germany.
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Table
Table 1. Spatio-temporal distribution of studies on A. baumannii of human origin in Germany from 2000 to 2018.
Table 1. Spatio-temporal distribution of studies on A. baumannii of human origin in Germany from 2000 to 2018.
RefFirst AuthorYr. of ReportSampling DateLocation of SamplingStudy PopulationSample TypeNo. Of Strains/CasesType of Study
[21](Katchanov et al., 2018)2018Sep. 2015 till Aug. 2016Hamburg-Eppendorf, Hamburg119 Individual patientsPneumonia and bloodstream infection18 patientsRetrospective analysis
[18](Wendel et al., 2018)2018Apr. 2017 till Mar. 2018Cologne, North Rhine-Westphalia44 Individual patientsRectal and nose/throat swabs, tracheal secretion and patients environment43/48 isolatesRetrospective analysis
[22](Kerz et al., 2018)2018NDMainz, Rhineland-PalatinateOne patientBlood culture and CSF1 caseCase report
[23](Papan et al., 2018)2018Feb. till Dec. 2015Munich, Bavaria29 Individual patientsWound, skin abscess, burn, synovial and thoracic drainage1 isolateRoutine bacterial diagnosis
[24](Lohr et al., 2018)2018Aug. 2016 till Jan. 2017NMC, Frankfurt/Main, Hesse67 Libyan war victimsRectal, nasopharyngeal and inguinal swabs and wounds15 isolatesPrevalence and investigation
[25](Tafelski et al., 2017)2017NDBerlin, Berlin1 patientCranial wound1 isolateCase report
[26](Rieber et al., 2017)2017Feb. 2008 till Jan. 2012North Rhine-Westphalia22/44 hospitalsRespiratory tract, wound, urine, vascular, joint, catheter, blood, intraoperative tissue, pharyngeal swabs79/1167 isolatesInvestigation study
[27](Reinheimer et al., 2016)2016Jun. till Dec. 2015UHF, Frankfurt/Main, Hesse143 refugee, 1489 residentsRectal swabs4 isolatesInvestigation study
[28](Pfeifer et al., 2016)2016Jul. 2012 till Sep. 2013Hospitals in Hesse and Bavaria2 Russian patientsIntraoperative wound swab3 isolates Case report
[29](Huenges et al., 2016)2016NDKiel, Schleswig-Holstein1 patientSkin swab and urine1 caseCase report
[30](Molter et al., 2016)20167 Dec. 2011 till 24 Jan. 2012LeverkusenOutbreak, 10 patientsThroat, groin, trachea10 casesOutbreak investigation
[31](Willems et al., 2016)2016Oct. 2013 till Mar. 2014Muenster, North Rhine-Westphalia2 ICUs at UHSkin, central intravenous catheter, respiratory samples32 isolatesRoutine surveillance study
[32](Ghebremedhin et al., 2016)2016NDUH Bochum and Wuppertal, North Rhine-WestphaliaStrain collection Culture isolates from laboratories38 isolatesDiagnostic study
[33](Hischebeth et al., 2015)2015NDUH Bonn, North Rhine-Westphalia1 patientHip joint infection1 isolateCase report
[34](Dersch et al., 2015)2015NDFreiburg, Baden-Württemberg1 patientCatheter associated ventriculitis/CSF1 isolateCase report
[35](Leistner et al., 2015)2015Jan. 2012 till Dec. 2013Berlin, Berlin213 Libyan patientsRectal and nasal swabs4 casesPrevalence and investigation
[36](Hauri et al., 2015)2015Jan. 2012 till Sep. 2014HesseLaboratories informationNotification cases185 complexData survey
[17](Siemers et al., 2014)201424 Dec. 2012 till 16 Jan. 2013Burn center in Halle (Saale), Saxony-Anhalt Outbreak, 7 patients Nasal and throat swabs, blood, skin and wounds7 casesOutbreak investigation
[19](Gottig et al., 2014)2014Dec. 2013Frankfurt, Hesse1 patientSkin and rectal swab1 PDR strainCase report
[37](Lahmer et al., 2014)2014NDMunich, BavariaOutbreak, 6 patientsBlood culture, bronchoalveolar lavage and ascites6 casesOutbreak investigation
[38](Kleinkauf et al., 2014)2014Apr. 2012 till Mar. 2013Frankfurt/Main, HesseHealth care facilitiesNotification cases, mainly wound swabs28 casesDescriptive analysis of data
[39](Schleicher et al., 2013)20132005, 2007 and 2009ND15 Diagnostic laboratoriesRespiratory tract, wound, intra-abdominal, blood culture140 isolatesSurveillance study
[40](Kaase, 2012a)20122011multi-centric study, NRL for MDRGNB, BochumStrain collectionRespiratory, wound, urine and blood culture292 isolatesSurveillance study
[41](Kaase, 2012b)20122011Nationwide (16 States), NRL for MDRGNB, BochumStrain collectionND287 isolatesSurveillance study
[42](Pfeifer et al., 2011)20112007ND1 patientND1 isolate 161/07Investigation study
[43](Wagner et al., 2011)2011NDLeipzig, Saxony1 patientDeep wound1 isolateCase report
[44](Higgins et al., 2010)2010NDCologne UH, North Rhine-Westphalia1 patientRespiratory, blood and bedsore33 isolatesCase report
[45](Aivazova et al., 2010)2010NDLMU, Munich, Bavaria1 patientVaginal smear1 isolateCase report
[46](Wadl et al., 2010)20102002 till 2006Thuringia, Baden-Wuerttemberg, Hesse, Lower Saxony, Saxony, Schleswig– Holstein4 from 6 University hospitalsRespiratory, blood, urine, non-specific1190 isolatesRetrospective and descriptive analysis of data
[47](Gottig et al., 2010)20102007UHF, Frankfurt, Hesse1 patientSkin, tracheal secretion, vessels prosthesis and woundNDCase report
[15](Frickmann et al., 2010)2010NDUH Rostock, Mecklenburg-VorpommernOutbreak, 7 patientsMedical devices, patient associated objectives, room equipment, urine, blood, testicular smear, lower respiratory, buccal swab, skin lesions13 isolatesOutbreak investigation
[48](Pfeifer, 2010)20102007South-Western GermanyHospitalNDTen isolatesCase report
[49](Seifert and Dowzicky, 2009)20092004–2007Throug out Germany13 CentersND187 isolatesEvaluation of antibiotic activities
[50](Kresken et al., 2009)20092007Throug out Germany15 CentersPeritoneal cavity, respiratory, blood, urine and wound117 isolatesEvaluation of antibiotic activities
[16](Kohlenberg et al., 2009)200918 Jun till 31 Aug. 2006NDOutbreak, 39 patientsWound, lower respiratory (one from each infusion pump, oxygen mask, hand of health care worker)39 isolatesOutbreak investigation
[51](Rodloff et al., 2008)2008Jan. 2004 till Aug. 2006Throug out Germany7 Medical centersBlood, wound, skin, urine, respiratory86 isolatesEvaluation of antibiotic activities
[52](Weyrich et al., 2006)2006NDUH, Tuebingen, Baden-Württemberg1 patientBlood culture1 caseCase report
[53](Schulte et al., 2005)2005NDSouth-Western Germany2 patientsBlood culture, excised tissue from fracture patella2 isolatesCase report
[54](Brauers et al., 2005)2005NDThroug out Germany + HU in Cologne, Freiburg, Leipzig13 LaboratoriesBlood culture, respiratory and urine395 isolatesEvaluation of antibiotic activities
[14](Borgmann et al., 2004)2004Feb. until Mar. 2003UH, Tuebingen, Baden-WürttembergOutbreak, 14 patientsWound, skin, respiratory250 isolatesOutbreak investigation
[55](Higgins et al., 2004b)20041991–1992Cologne, North Rhine-Westphalia2 Hospital outbreaksUrine, blood culture34 casesOutbreak investigation
[56](Frank et al., 2003)2003NDThroug out Germany19 LaboratoriesUrinary, respiratory tract, wound, blood51 isolatesEvaluation of antibiotic activities
[57](Andermahr et al., 2002)2002Jun. 1996 till Jun 2001Cologne, North Rhine-Westphalia266 patientsTracheal aspirate41 casesRoutine bacterial diagnosis
[58](Heinemann et al., 2000)2000Jul. 1990 till Dec. 1998Cologne, North Rhine-WestphaliaOutbreaks and sporadic casesBlood, urine, wound, tracheal secretion47 isolatesEvaluation of antibiotic activities
ND: not determined, MDR: Multidrug-resistant, MIC: minimum inhibitory concentrations, UH: university hospital.
Table
Table 2. Number of the nosocomial outbreaks of carbapenem-non-susceptible Acinetobacter spp. in Germany based on official data published by the Robert Koch Institute (RKI) (2010–2018).
Table 2. Number of the nosocomial outbreaks of carbapenem-non-susceptible Acinetobacter spp. in Germany based on official data published by the Robert Koch Institute (RKI) (2010–2018).
Year of ReportNo. of OutbreaksNo. of CasesNo. of DeathsNo. of InfectionIncidence
201851857840.9
2017741117941.0
201610388--
201585627--
20146291--
201314674--
201213869--
2011-----
2010-----
Table
Table 3. Temporal distribution of resistance profiles of A. baumannii strains from Germany based on studies published from 2000 to 2018.
Table 3. Temporal distribution of resistance profiles of A. baumannii strains from Germany based on studies published from 2000 to 2018.
No.ReferencesSampling DateResistant AntibioticsSusceptible AntibioticsResistance GenesST/IC
[21](Katchanov et al., 2018)Sep. 2015 till Aug. 2016MDR, carbapenem-resistantColistin in all tested ten isolatesTen isolates carry blaOXA-23ND
[18](Wendel et al., 2018)Apr. 2017 till Mar. 2018Two strains were carbapenem-resistantCarbapenem-2.1. Historical glance on the situation in humanCephalosporinase-encoding blaADC-25-like, blaOXA-106-like, blaOXA-51ND
[22](Kerz et al., 2018)NDCarbapenemase-producing (Meropenem > 32 mg/L)Colistin < 1 mg/LblaOXA-23 and blaNDM-1ND
[23](Papan et al., 2018)Feb. till Dec. 2015One strain was carbapenem-resistantNDblaOXA-24/40ND
[24](Lohr et al., 2018)Aug. 2016 till Jan. 2017Carbapenemase-producingNDblaOXA-23 (n = 9), blaNDM (n = 2), blaNDM+OXA-51-ISAbaI (n = 4), TEM and PMQR gene aac(6`)-Ib-crND
[25](Tafelski et al., 2017)NDMDR, imipenem, meropenem ceftriaxone, amikacin, cotrimoxazole, fosfomycin, ciprofloxacin, levofloxacin.Colistin < 0.5 mg/L and tobramycinNDND
[26](Rieber et al., 2017)Feb. 2008 till Jan. 2012ESBL, Carbapenem-resistant, ciprofloxacin and gentamicinPolymyxin BblaOXA-23 (n = 66), blaOXA-80 (n = 1), blaOXA-82 (n = 3), TEM-like (n = 62), GES-11 (n = 1), PER-1 (n = 1); blaOXA-51-like (OXA-51, OXA-66, OXA-69)ND
[27](Reinheimer et al., 2016)Jun. till Dec. 2015Three strains were carbapenem-resistant from refugee and one from residentsNDblaOXA-23 and blaOXA-24 in refugee and blaOXA-51 in residentIC-2
[28](Pfeifer et al., 2016)Jul. 2012 till Sep. 2013Penicillin/inhibitor combinations, cefepime, imipenem, meropenem, aminoglycosides (gentamicin, tobramycin and amikacin), fluoroquinolones (ciprofloxacin) and sulfamethoxazole/trimethoprim.ColistinblaOXA-40/24 related blaOXA-72, blaOXA-66, blaOXA-90, ESBL-blaCTX-M-2-like genes and blaCTX-M-115IC-2, IC-6, ST944/ST78 ST348/ST2
[29](Huenges et al., 2016)ND4MRGN-ab, intermediate to carbapenemsColistinNDND
[30](Molter et al., 2016)7 Dec. 2011 till 24 Jan. 2012Carbapenems, ciprofloxacin and tobramycin; variable to tigecycline (≤0.5 to 4 mg/L) and amikacin (4 to ≥ 64 mg/L).Colistin (MIC 0.5–1 mg/L)blaOXA-51-like and blaOXA-23-likeIC-2, ST195 and ST218
[31](Willems et al., 2016)Oct. 2013 till Mar. 2014MDR, piperacillin, 3rd/4th generation cephalosporins and fluoroquinolonesCarbapenems, imipenem and meropenemNDND
[32](Ghebremedhin et al., 2016)NDCarbapenemase-producingNDblaOXA-23, blaOXA-40, blaOXA-58, blaOXA-72, blaOXA-164, blaNDM-1, GIM-1, VIP-2 and VIM-2ND
[33](Hischebeth et al., 2015)NDCarbapenemase-producing, meropenem (MIC ≥ 32 mg/L) and imipenem (MIC ≥ 32 mg/L), tobramycin (MIC ≥ 16 mg/L) gentamicin (MIC ≥ 16 mg/L)Colistin only (MIC ≤ 0.5 mg/L)blaOXA-23-like and blaOXA-51-like.IC-2
[34](Dersch et al., 2015)NDMDRColistinNDND
[35](Leistner et al., 2015)Jan. 2012 till Dec. 2013ESBL, Carbapenem-resistant, Imipenem and Meropenem (MIC ≥ 16 mg/L), Ertapenem (MIC ≥ 32 mg/L).NDblaOXA-23, TEM-1, GES-11; PMQR genes (aac(6´)1b-cr)ND
[36](Hauri et al., 2015)Jan. 2012 till Sep. 20144MRGN, acylureidopenicillins, 3rd/4th generation cephalosporins, carbapenems, fluoroquinolone.NDblaOXA-23 and blaNDMND
[17](Siemers et al., 2014)24 Dec. till 2012 to 16 Jan. 20134MRGN, acylureidopenicillins, 3rd/4th generation cephalosporins, carbapenems, fluoroquinolone.ColistinblaOXA-58ND
[19](Gottig et al., 2014)Dec. 2013PDR, penicillins/β-lactamase inhibitors, extended-spectrum cephalosporins, carbapenems, tetracyclines, fluoroquinolones, aminoglycosides, folate pathway inhibitors and polymyxins, including colistin (MIC > 128 mg/L) and tigecycline (MIC > 8 mg/L), chloramphenicol and fosfomycinIntermediately susceptible to minocycline (MIC 8 mg/L)BlaOXA-23, blaOXA-51-like (blaOXA-61)IC-2
[37](Lahmer et al., 2014)NDMDR, Carbapenem-resistantTigecycline and colistinNDND
[38](Kleinkauf et al., 2014)Apr. 2012 till Mar. 2013Carbapenem-resistant blaOXA-23-like and blaNDM-1ND
[39](Schleicher et al., 2013)2005, 2007 and 2009Non-susceptible to carbapenem and imipenemNDblaOXA-58-like and blaOXA-23-like.IC-2, IC-1, IC-4, IC-7
[40](Kaase, 2012a)2011Carbapenem-resistant, imipenem and meropenem,NDblaOXA-23, blaOXA-72, blaOXA-58, blaOXA-164, NDM-1, ISAba1 insertion blaOXA-51 and GES-11ND
[41](Kaase, 2012b)2011Carbapenem-resistant, imipenem and meropenem,NDblaOXA-23, blaOXA-72, blaOXA-58, blaOXA-164, NDM-1, ISAba1 insertion blaOXA-51 and GES-11ND
[42](Pfeifer et al., 2011)2007MDR, imipenem and meropenem, ertapenem (MIC >32 mg/L), tigecycline (MIC1 mg/L)Colistin (MIC 0.38 mg/L)blaNDM-1 and blaOXA-64IC-7
[43](Wagner et al., 2011)NDMDR, ampicillin + sulbactam, piperacillin + tazobactam, cefuroxime, cefotaxime, ceftriaxone, ceftazidime, aztreonam, ertapenem, amikacin, fosfomycin, levofloxacin, ciprofloxacin and moxifloxacin.Imipenem and tobramycinNDND
[44](Higgins et al., 2010)NDCefepime, cefotaxime, cefoxitin, ceftazidime, ciprofloxacin, moxifloxacin, fosfomycin, gentamicin, piperacillin, piperacillin-tazobactam and ampicillin-sulbactam, imipenem, meropenem. Amikacin variable (MIC ≤ 2 and ≥ 32 mg/L), tigecycline variable (MIC 2 and ≥ 8 µg/mL).Colistin (MIC ≤1 µg/mL) and tobramycin (MIC ≤ 1 µg/mL)blaOXA-51-like (blaOXA-69), blaOXA-58-like variant (novel blaOXA-164 and blaOXA-58). adeB, adeJ, novel adeR mutationIC-1
[45](Aivazova et al., 2010)NDNDMeropenemNDND
[46](Wadl et al., 2010)2002 till 200666 are MDR, beta-lactams (piperacillin/ tazobactam), fluoroquinolones (ciprofloxacin, levofloxacin), cephalosporins (ceftazidime, cefepime), Aminoglycosides (tobramycin, gentamicin) and trimethoprim/ sulphamethoxazole combination cotrimoxazole.Lowest resistance in carbapenems (meropenem, imipenem) and beta-lactams (ampicillin/sulbactam).NDND
[47](Gottig et al., 2010)2007Carbapenems, fluoroquinolones, aminoglycosides, tigecycline, aztreonam,Colistin (MIC ≤ 1 µg/mL)blaNDM-1ND
[15](Frickmann et al., 2010)NDMDR (ampicillin-sulbactam, piperacillin-tazobactam, cefuroxime, cefotaxime, cefpodoxime, ceftazidime, imipenem, meropenem, ciprofloxacin, levofloxacin, gentamycin, tobramycin, tetracycline, trimethoprim-sulfamethoxazole, tigecycline, doripenem, cefepime, ertapenem, moxifloxacin, fosfomycin, aztreonam).ColistinNDST22, ST53
[48](Pfeifer, 2010)2007MDR, carbapenemsColistin (MIC 0.25 µg/mL)blaNDM-1ND
[49](Seifert and Dowzicky, 2009)2004–2007Ampicillin and amoxicillin/clavulanic acid (MIC ≥ 64 µg/mL), ceftriaxone (MIC ≥ 128 µg/mL) and cefepime (MIC 32 µg/mL)Tigecycline, amikacin and minocycline (MIC ≤1 µg/mL), imipenem, meropenem, levofloxacin,NDND
[50](Kresken et al., 2009)2007Imipenem (11.1%), ciprofloxacin (27.4%), gentamycin (22.2%)Tigecycline (MIC ≤ 0.25/1 µg/mL)NDND
[16](Kohlenberg et al., 2009)18 Jun till 31 Aug. 2006Carbapenem-resistant, penicillins, cephalosporins, ciprofloxacin, gentamicin, tobramycin, tigecycline (4 mg/L), imipenem and meropenem (MIC ≥ 32 mg/L)Colistin (MIC ≤ 0.5 mg/L) and amikacin (MIC 4–8 mg/L). Two isolates susceptible to imipenemblaOXA-23-like, blaOXA-58-like intrinsic blaOXA-51IC-2
[51](Rodloff et al., 2008)Jan. 2004 till Aug. 2006NDTigecycline (MIC ≤ 1 mg/L), >80% for amikacin, cefepime, ceftazidime, imipenem (MIC 1 mg/L), levofloxacin, minocycline, piperacillin-tazobactam.NDND
[52](Weyrich et al., 2006)NDMDR, penicillins, cephalosporins, aminoglycosides, fluoroquinolones, rifampicin, tetracyclines, oxazolidinones, macrolides and carbapenems (imipenem and imipenem/EDTAColistinMetallo-ß-lactamaseND
[53](Schulte et al., 2005)NDCarbapenems, meropenem, penicillins, cephalosporins, aminoglycosides, quinolonesNDMetallo-ß-lactamaseND
[54](Brauers et al., 2005)NDMIC ≥ 64 mg/L in amoxicillin-clavulanate combinations, piperacillin-tazobactam and cefotaximeAmpicillin-sulbactam and piperacillin-sulbactam (MIC ≤ 0.06 mg/L), meropenem (MIC ≤ 1 mg/L)NDND
[14](Borgmann et al., 2004)Feb. until Mar. 2003MDR, gentamicin, aztreonam, piperacillin, piperacillin/ tazobactam, cefuroxime, cefotaxime, ceftazidime, cefepime, meropenem, tetracycline, levofloxacin, ciprofloxacin and fosfomycinTobramycin, amikacin and colistinMetallo-ß-lactamaseND
[55](Higgins et al., 2004b)1991–1992Ciprofloxacin, ofloxacin, meropenem, netilmicin, tetracycline, tobramycin, clarithromycinNDgyrA and parC mutation, up-regulation of adeB geneND
[56](Frank et al., 2003)NDNDPiperacillin-sulbactamNDND
[57](Andermahr et al., 2002)Jun. 1996 till Jun 2001NDNDNDND
[58](Heinemann et al., 2000)Jul. 1990 till Dec. 1998Novel quinolones superior to ciprofloxacinClinafloxacin most active NDND
ND: not determined, MDR: Multidrug-resistant, MIC: minimum inhibitory concentrations, ST/IC: sequence type and international clone.

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Wareth, G.; Brandt, C.; Sprague, L.D.; Neubauer, H.; Pletz, M.W. Spatio-Temporal Distribution of Acinetobacter baumannii in Germany—A Comprehensive Systematic Review of Studies on Resistance Development in Humans (2000–2018). Microorganisms 2020, 8, 375. https://doi.org/10.3390/microorganisms8030375

AMA Style

Wareth G, Brandt C, Sprague LD, Neubauer H, Pletz MW. Spatio-Temporal Distribution of Acinetobacter baumannii in Germany—A Comprehensive Systematic Review of Studies on Resistance Development in Humans (2000–2018). Microorganisms. 2020; 8(3):375. https://doi.org/10.3390/microorganisms8030375

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Wareth, Gamal, Christian Brandt, Lisa D. Sprague, Heinrich Neubauer, and Mathias W. Pletz. 2020. "Spatio-Temporal Distribution of Acinetobacter baumannii in Germany—A Comprehensive Systematic Review of Studies on Resistance Development in Humans (2000–2018)" Microorganisms 8, no. 3: 375. https://doi.org/10.3390/microorganisms8030375

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Wareth, G., Brandt, C., Sprague, L. D., Neubauer, H., & Pletz, M. W. (2020). Spatio-Temporal Distribution of Acinetobacter baumannii in Germany—A Comprehensive Systematic Review of Studies on Resistance Development in Humans (2000–2018). Microorganisms, 8(3), 375. https://doi.org/10.3390/microorganisms8030375

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