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Open AccessArticle

Alteration of Bacterial Communities in Anterior Nares and Skin Sites of Patients Undergoing Arthroplasty Surgery: Analysis by 16S rRNA and Staphylococcal-Specific tuf Gene Sequencing

1
Department of Bacteria, Parasites and Fungi, Statens Serum Institut, 2300 Copenhagen, Denmark
2
Department of Infectious Diseases and Centre for Clinical Research and Education, County Council of Värmland, SE-651 82 Karlstad, Sweden
3
School of Medical Sciences, Faculty of Medicine and Health, Örebro University, SE-701 82 Örebro, Sweden
4
Centre for Clinical Research, Hospital Västmanland, Uppsala University, Region Västmanland, SE‐721 89 Västerås, Sweden
5
Department of Infectious Diseases and Department of Clinical and Biomedical Sciences, Linköping University, SE-581 83 Norrköping, Sweden
6
Department of Laboratory Medicine, Clinical Microbiology, Faculty of Medicine and Health, Örebro University, SE-701 82 Örebro, Sweden
*
Author to whom correspondence should be addressed.
These authors share senior authorship.
Microorganisms 2020, 8(12), 1977; https://doi.org/10.3390/microorganisms8121977
Received: 24 November 2020 / Revised: 7 December 2020 / Accepted: 8 December 2020 / Published: 12 December 2020
The aim was to study alterations of bacterial communities in patients undergoing hip or knee arthroplasty to assess the impact of chlorhexidine gluconate soap decolonisation and systemic antibiotic prophylaxis. A Swedish multicentre, prospective collection of samples obtained from elective arthroplasty patients (n = 83) by swabbing anterior nares, skin sites in the groin and the site of planned surgery, before and after arthroplasty surgery, was analysed by 16S rRNA (V3-V4) gene sequencing and a complementary targeted tuf gene sequencing approach to comprehensively characterise alterations in staphylococcal communities. Significant reductions in alpha diversity was detected for both bacterial (p = 0.04) and staphylococcal (p = 0.03) groin communities after arthroplasty surgery with significant reductions in relative Corynebacterium (p = 0.001) abundance and Staphylococcus hominis (p = 0.01) relative staphylococcal abundance. In nares, significant reductions occurred for Staphylococcus hominis (p = 0.02), Staphylococcus haemolyticus (p = 0.02), and Staphylococcus pasteuri (p = 0.003) relative to other staphylococci. Staphylococcus aureus colonised 35% of anterior nares before and 26% after arthroplasty surgery. Staphylococcus epidermidis was the most abundant staphylococcal species at all sampling sites. No bacterial genus or staphylococcal species increased significantly after arthroplasty surgery. Application of a targeted tuf gene sequencing approach provided auxiliary staphylococcal community profiles and allowed species-level characterisation directly from low biomass clinical samples. View Full-Text
Keywords: arthroplasty; prosthetic joint replacement; prosthetic joint infection; systemic antibiotic prophylaxis; antibiotics; chlorhexidine gluconate; coagulase-negative staphylococci; tuf gene sequencing; staphylome; 16S rRNA gene sequencing; microbiome arthroplasty; prosthetic joint replacement; prosthetic joint infection; systemic antibiotic prophylaxis; antibiotics; chlorhexidine gluconate; coagulase-negative staphylococci; tuf gene sequencing; staphylome; 16S rRNA gene sequencing; microbiome
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MDPI and ACS Style

Iversen, S.; Johannesen, T.B.; Ingham, A.C.; Edslev, S.M.; Tevell, S.; Månsson, E.; Nilsdotter-Augustinsson, Å.; Söderquist, B.; Stegger, M.; Andersen, P.S. Alteration of Bacterial Communities in Anterior Nares and Skin Sites of Patients Undergoing Arthroplasty Surgery: Analysis by 16S rRNA and Staphylococcal-Specific tuf Gene Sequencing. Microorganisms 2020, 8, 1977. https://doi.org/10.3390/microorganisms8121977

AMA Style

Iversen S, Johannesen TB, Ingham AC, Edslev SM, Tevell S, Månsson E, Nilsdotter-Augustinsson Å, Söderquist B, Stegger M, Andersen PS. Alteration of Bacterial Communities in Anterior Nares and Skin Sites of Patients Undergoing Arthroplasty Surgery: Analysis by 16S rRNA and Staphylococcal-Specific tuf Gene Sequencing. Microorganisms. 2020; 8(12):1977. https://doi.org/10.3390/microorganisms8121977

Chicago/Turabian Style

Iversen, Søren; Johannesen, Thor B.; Ingham, Anna C.; Edslev, Sofie M.; Tevell, Staffan; Månsson, Emeli; Nilsdotter-Augustinsson, Åsa; Söderquist, Bo; Stegger, Marc; Andersen, Paal S. 2020. "Alteration of Bacterial Communities in Anterior Nares and Skin Sites of Patients Undergoing Arthroplasty Surgery: Analysis by 16S rRNA and Staphylococcal-Specific tuf Gene Sequencing" Microorganisms 8, no. 12: 1977. https://doi.org/10.3390/microorganisms8121977

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