Integrated Stirred-Tank Bioreactor with Internal Adsorption for the Removal of Ammonium to Enhance the Cultivation Performance of gdhA Derivative Pasteurella multocida B:2

Oslan, Siti Nur Hazwani; Tan, Joo Shun; Abbasiliasi, Sahar; Ziad Sulaiman, Ahmad; Saad, Mohd Zamri; Halim, Murni; Ariff, Arbakariya B.

doi:10.3390/microorganisms8111654

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Integrated Stirred-Tank Bioreactor with Internal Adsorption for the Removal of Ammonium to Enhance the Cultivation Performance of gdhA Derivative Pasteurella multocida B:2

Siti Nur Hazwani Oslan

^1,2

³,

⁴,

²,

⁵,

⁶

and

Arbakariya B. Ariff

^1,6,*

Bioprocessing and Biomanufacturing Research Centre, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia

Faculty of Bioengineering and Technology, University Malaysia Kelantan, Jeli Campus, Jeli, Kelantan 17600, Malaysia

Bioprocess Technology, School of Industrial Technology, Universiti Sains Malaysia, Penang 11800, Malaysia

⁴

Halal Products Research Institute, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia

⁵

Research Centre for Ruminant Diseases, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia

⁶

Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia

Author to whom correspondence should be addressed.

Microorganisms 2020, 8(11), 1654; https://doi.org/10.3390/microorganisms8111654

Received: 10 September 2020 / Revised: 6 October 2020 / Accepted: 6 October 2020 / Published: 24 October 2020

(This article belongs to the Special Issue Microbial Secondary Metabolites and Biotechnology)

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Abstract

Growth of mutant gdhA Pasteurella multocida B:2 was inhibited by the accumulation of a by-product, namely ammonium in the culture medium during fermentation. The removal of this by-product during the cultivation of mutant gdhA P. multocida B:2 in a 2 L stirred-tank bioreactor integrated with an internal column using cation-exchange adsorption resin for the improvement of cell viability was studied. Different types of bioreactor system (dispersed and internal) with resins were successfully used for ammonium removal at different agitation speeds. The cultivation in a bioreactor integrated with an internal column demonstrated a significant improvement in growth performance of mutant gdhA P. multocida B:2 (1.05 × 10¹¹ cfu/mL), which was 1.6-fold and 8.4-fold as compared to cultivation with dispersed resin (7.2 × 10¹⁰ cfu/mL) and cultivation without resin (1.25 × 10¹⁰ cfu/mL), respectively. The accumulation of ammonium in culture medium without resin (801 mg/L) was 1.24-fold and 1.37-fold higher than culture with dispersed resin (642.50 mg/L) and culture in the bioreactor integrated with internal adsorption (586.50 mg/L), respectively. Results from this study demonstrated that cultivation in a bioreactor integrated with the internal adsorption column in order to remove ammonium could reduce the inhibitory effect of this by-product and improve the growth performance of mutant gdhA P. multocida B:2. View Full-Text

Keywords: cation-exchange resin; adsorption; removal; ammonium; cell viability; mutant gdhA P. multocida B:2

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Oslan, S.N.H.; Tan, J.S.; Abbasiliasi, S.; Ziad Sulaiman, A.; Saad, M.Z.; Halim, M.; Ariff, A.B. Integrated Stirred-Tank Bioreactor with Internal Adsorption for the Removal of Ammonium to Enhance the Cultivation Performance of gdhA Derivative Pasteurella multocida B:2. Microorganisms 2020, 8, 1654. https://doi.org/10.3390/microorganisms8111654

AMA Style

Oslan SNH, Tan JS, Abbasiliasi S, Ziad Sulaiman A, Saad MZ, Halim M, Ariff AB. Integrated Stirred-Tank Bioreactor with Internal Adsorption for the Removal of Ammonium to Enhance the Cultivation Performance of gdhA Derivative Pasteurella multocida B:2. Microorganisms. 2020; 8(11):1654. https://doi.org/10.3390/microorganisms8111654

Chicago/Turabian Style

Oslan, Siti N.H., Joo S. Tan, Sahar Abbasiliasi, Ahmad Ziad Sulaiman, Mohd Z. Saad, Murni Halim, and Arbakariya B. Ariff. 2020. "Integrated Stirred-Tank Bioreactor with Internal Adsorption for the Removal of Ammonium to Enhance the Cultivation Performance of gdhA Derivative Pasteurella multocida B:2" Microorganisms 8, no. 11: 1654. https://doi.org/10.3390/microorganisms8111654

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Integrated Stirred-Tank Bioreactor with Internal Adsorption for the Removal of Ammonium to Enhance the Cultivation Performance of gdhA Derivative Pasteurella multocida B:2

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