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Microorganisms
Volume 8
Issue 10
10.3390/microorganisms8101525
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Article

Proteotyping Environmental Microorganisms by Phylopeptidomics: Case Study Screening Water from a Radioactive Material Storage Pool

by
Karim Hayoun
1,2,
Olivier Pible
1,
Pauline Petit
3,
François Allain
1,
Virginie Jouffret
1,
Karen Culotta
1,
Corinne Rivasseau
4,
Jean Armengaud
1,* and
Béatrice Alpha-Bazin
1
1
Département Médicaments et Technologies pour la Santé (DMTS), CEA, INRAE, SPI, Université Paris Saclay, F-30200 Bagnols-sur-Cèze, France
2
Laboratoire Innovations technologiques pour la Détection et le Diagnostic (Li2D), Université de Montpellier, F-30207 Bagnols-sur-Cèze, France
3
CEA, CNRS, INRA, Université Grenoble Alpes, Institut de Biosciences et Biotechnologies de Grenoble, UMR5168, F-38000 Grenoble, France
4
CEA-Saclay, DRF/Joliot/SB2SM/BBC, I2BC, 91191 Gif-sur-Yvette, France
*
Author to whom correspondence should be addressed.
Microorganisms 2020, 8(10), 1525; https://doi.org/10.3390/microorganisms8101525
Submission received: 22 August 2020 / Revised: 30 September 2020 / Accepted: 1 October 2020 / Published: 4 October 2020
(This article belongs to the Section Systems Microbiology)
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Abstract

The microbial diversity encompassed by the environmental biosphere is largely unexplored, although it represents an extensive source of new knowledge and potentially of novel enzymatic catalysts for biotechnological applications. To determine the taxonomy of microorganisms, proteotyping by tandem mass spectrometry has proved its efficiency. Its latest extension, phylopeptidomics, adds a biomass quantitation perspective for mixtures of microorganisms. Here, we present an application of phylopeptidomics to rapidly and sensitively screen microorganisms sampled from an industrial environment, i.e., a pool where radioactive material is stored. The power of this methodology is demonstrated through the identification of both prokaryotes and eukaryotes, whether as pure isolates or present as mixtures or consortia. In this study, we established accurate taxonomical identification of environmental prokaryotes belonging to the Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria phyla, as well as eukaryotes from the Ascomycota phylum. The results presented illustrate the potential of tandem mass spectrometry proteotyping, in particular phylopeptidomics, to screen for and rapidly identify microorganisms.
Keywords: microorganisms; tandem mass spectrometry; proteotyping; screening; taxonomy microorganisms; tandem mass spectrometry; proteotyping; screening; taxonomy
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MDPI and ACS Style

Hayoun, K.; Pible, O.; Petit, P.; Allain, F.; Jouffret, V.; Culotta, K.; Rivasseau, C.; Armengaud, J.; Alpha-Bazin, B. Proteotyping Environmental Microorganisms by Phylopeptidomics: Case Study Screening Water from a Radioactive Material Storage Pool. Microorganisms 2020, 8, 1525. https://doi.org/10.3390/microorganisms8101525

AMA Style

Hayoun K, Pible O, Petit P, Allain F, Jouffret V, Culotta K, Rivasseau C, Armengaud J, Alpha-Bazin B. Proteotyping Environmental Microorganisms by Phylopeptidomics: Case Study Screening Water from a Radioactive Material Storage Pool. Microorganisms. 2020; 8(10):1525. https://doi.org/10.3390/microorganisms8101525

Chicago/Turabian Style

Hayoun, Karim, Olivier Pible, Pauline Petit, François Allain, Virginie Jouffret, Karen Culotta, Corinne Rivasseau, Jean Armengaud, and Béatrice Alpha-Bazin. 2020. "Proteotyping Environmental Microorganisms by Phylopeptidomics: Case Study Screening Water from a Radioactive Material Storage Pool" Microorganisms 8, no. 10: 1525. https://doi.org/10.3390/microorganisms8101525

APA Style

Hayoun, K., Pible, O., Petit, P., Allain, F., Jouffret, V., Culotta, K., Rivasseau, C., Armengaud, J., & Alpha-Bazin, B. (2020). Proteotyping Environmental Microorganisms by Phylopeptidomics: Case Study Screening Water from a Radioactive Material Storage Pool. Microorganisms, 8(10), 1525. https://doi.org/10.3390/microorganisms8101525

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