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Article

Pepper Bacterial Spot Control by Bacillus velezensis: Bioprocess Solution

1
Faculty of Technology, University of Novi Sad, Bulevar cara Lazara 1, 21000 Novi Sad, Serbia
2
Technical University of Applied Sciences Wildau, Hochschulring 1, 15745 Wildau, Germany
3
Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000 Novi Sad, Serbia
4
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade, Vojvode Stepe 444a, P. Fah 23, 11010 Belgrade, Serbia
*
Authors to whom correspondence should be addressed.
Microorganisms 2020, 8(10), 1463; https://doi.org/10.3390/microorganisms8101463
Received: 14 August 2020 / Revised: 4 September 2020 / Accepted: 5 September 2020 / Published: 24 September 2020
Pepper bacterial spot is one of the most severe plant diseases in terms of infection persistence and economic losses when it comes to fresh pepper fruits used in nutrition and industrial processing. In this study, Bacillus velezensis IP22 isolated from fresh cheese was used as a biocontrol agent of pepper bacterial spot, whose main causal agent is the cosmopolitan pathogen Xanthomonas euvesicatoria. After optimization of the cultivation medium composition aimed at maximizing of the antimicrobial activity against X. euvesicatoria and validation of the optimized medium at the scale of a laboratory bioreactor, in planta tests were performed. The results have showed significant suppression of bacterial spot symptoms in pepper plants by the produced biocontrol agent, as well as reduction of disease spreading on the healthy (uninoculated) pepper leaves. Furthermore, HPLC-MS (high pressure liquid chromatography–mass spectrometry) analysis was employed to examine antimicrobial metabolites produced by B. velezensis IP22, where lipopeptides were found with similar m/z values compared to lipopeptides from fengycin and locillomycin families. The bioprocess solution developed at the laboratory scale investigated in this study represents a promising strategy for production of pepper bacterial spot biocontrol agent based on B. velezensis IP22, a food isolate with a great perspective for application in plant protection. View Full-Text
Keywords: Xanthomonas euvesicatoria; biological control; biocontrol agent; medium optimization; lipopeptides; fengycin; locillomycin; HPLC-MS; bioreactor; laboratory scale Xanthomonas euvesicatoria; biological control; biocontrol agent; medium optimization; lipopeptides; fengycin; locillomycin; HPLC-MS; bioreactor; laboratory scale
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MDPI and ACS Style

Pajčin, I.; Vlajkov, V.; Frohme, M.; Grebinyk, S.; Grahovac, M.; Mojićević, M.; Grahovac, J. Pepper Bacterial Spot Control by Bacillus velezensis: Bioprocess Solution. Microorganisms 2020, 8, 1463. https://doi.org/10.3390/microorganisms8101463

AMA Style

Pajčin I, Vlajkov V, Frohme M, Grebinyk S, Grahovac M, Mojićević M, Grahovac J. Pepper Bacterial Spot Control by Bacillus velezensis: Bioprocess Solution. Microorganisms. 2020; 8(10):1463. https://doi.org/10.3390/microorganisms8101463

Chicago/Turabian Style

Pajčin, Ivana, Vanja Vlajkov, Marcus Frohme, Sergii Grebinyk, Mila Grahovac, Marija Mojićević, and Jovana Grahovac. 2020. "Pepper Bacterial Spot Control by Bacillus velezensis: Bioprocess Solution" Microorganisms 8, no. 10: 1463. https://doi.org/10.3390/microorganisms8101463

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