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Open AccessArticle

The Transcription Factor Sfp1 Regulates the Oxidative Stress Response in Candida albicans

1
Institute of Molecular and Cellular Biology, National Tsing Hua University, Hsinchu 30013, Taiwan
2
Department of Life Science, National Tsing Hua University, Hsinchu 30013, Taiwan
*
Author to whom correspondence should be addressed.
Microorganisms 2019, 7(5), 131; https://doi.org/10.3390/microorganisms7050131
Received: 31 March 2019 / Revised: 7 May 2019 / Accepted: 9 May 2019 / Published: 14 May 2019
(This article belongs to the Special Issue Molecular Mechanisms of Fungal Virulence and Commensalism)
Candida albicans is a commensal that inhabits the skin and mucous membranes of humans. Because of the increasing immunocompromised population and the limited classes of antifungal drugs available, C. albicans has emerged as an important opportunistic pathogen with high mortality rates. During infection and therapy, C. albicans frequently encounters immune cells and antifungal drugs, many of which exert their antimicrobial activity by inducing the production of reactive oxygen species (ROS). Therefore, antioxidative capacity is important for the survival and pathogenesis of C. albicans. In this study, we characterized the roles of the zinc finger transcription factor Sfp1 in the oxidative stress response against C. albicans. A sfp1-deleted mutant was more resistant to oxidants and macrophage killing than wild-type C. albicans and processed an active oxidative stress response with the phosphorylation of the mitogen-activated protein kinase (MAPK) Hog1 and high CAP1 expression. Moreover, the sfp1-deleted mutant exhibited high expression levels of antioxidant genes in response to oxidative stress, resulting in a higher total antioxidant capacity, glutathione content, and glutathione peroxidase and superoxide dismutase enzyme activity than the wild-type C. albicans. Finally, the sfp1-deleted mutant was resistant to macrophage killing and ROS-generating antifungal drugs. Together, our findings provide a new understanding of the complex regulatory machinery in the C. albicans oxidative stress response. View Full-Text
Keywords: Candida albicans; Sfp1; oxidative stress response Candida albicans; Sfp1; oxidative stress response
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Lee, S.-Y.; Chen, H.-F.; Yeh, Y.-C.; Xue, Y.-P.; Lan, C.-Y. The Transcription Factor Sfp1 Regulates the Oxidative Stress Response in Candida albicans. Microorganisms 2019, 7, 131.

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