Next Article in Journal
Urbanization Altered Bacterial and Archaeal Composition in Tidal Freshwater Wetlands Near Washington DC, USA, and Buenos Aires, Argentina
Previous Article in Journal
MmpL Proteins in Physiology and Pathogenesis of M. tuberculosis
Article Menu
Issue 3 (March) cover image

Export Article

Open AccessArticle
Microorganisms 2019, 7(3), 71; https://doi.org/10.3390/microorganisms7030071

Bacillus subtilis DSM 32315 Supplementation Attenuates the Effects of Clostridium perfringens Challenge on the Growth Performance and Intestinal Microbiota of Broiler Chickens

1
Department of Poultry Science, University of Georgia, Athens, GA 30602, USA
2
Evonik Nutrition & Care GmbH, 63457 Hanau, Germany
3
Evonik Corporation, Kennesaw, GA 30144, USA
*
Authors to whom correspondence should be addressed.
Received: 6 February 2019 / Revised: 23 February 2019 / Accepted: 4 March 2019 / Published: 5 March 2019
(This article belongs to the Special Issue Gut Health in Poultry Production)
  |  
PDF [1277 KB, uploaded 9 March 2019]
  |  

Abstract

The objective of this study was to evaluate the effects of the dietary supplementation of Bacillus subtilis DSM 32315 (probiotic) on the performance and intestinal microbiota of broiler chickens infected with Clostridium perfringens (CP). One-day-old broiler chickens were assigned to 3 treatments with 8 replicate pens (50 birds/pen). The treatments were: non-infected control; infected control; and infected supplemented with probiotic (1 × 106 CFU/g of feed). On day of hatch, all birds were sprayed with a coccidia vaccine based on the manufacturer recommended dosage. On d 18–20 the infected birds were inoculated with CP via feed. Necrotic enteritis (NE) lesion score was performed on d 21. Digestive tract of 2 birds/pen was collected on d 21 to analyze the ileal and cecal microbiota by 16S rRNA sequencing. Performance was evaluated on d 28 and 42. On d 21, probiotic supplementation reduced (p < 0.001) the severity of NE related lesion versus infected control birds. On d 28, feed efficiency was improved (p < 0.001) in birds supplemented with probiotic versus infected control birds. On d 42, body weight gain (BW gain) and feed conversion ratio (FCR) were improved (p < 0.001) in probiotic supplemented birds versus infected control birds. The diversity, composition and predictive function of the intestinal microbial digesta changed with the infection but the supplementation of probiotic reduced these variations. Therefore, dietary supplementation of Bacillus subtilis DSM 32315 was beneficial in attenuating the negative effects of CP challenge on the performance and intestinal microbiota of broilers chickens. View Full-Text
Keywords: Bacillus subtilis; broiler; microbiota; necrotic enteritis Bacillus subtilis; broiler; microbiota; necrotic enteritis
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Bortoluzzi, C.; Serpa Vieira, B.; de Paula Dorigam, J.C.; Menconi, A.; Sokale, A.; Doranalli, K.; Applegate, T.J. Bacillus subtilis DSM 32315 Supplementation Attenuates the Effects of Clostridium perfringens Challenge on the Growth Performance and Intestinal Microbiota of Broiler Chickens. Microorganisms 2019, 7, 71.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Microorganisms EISSN 2076-2607 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top