Next Article in Journal
Requirement of lmo1930, a Gene in the Menaquinone Biosynthesis Operon, for Esculin Hydrolysis and Lithium Chloride Tolerance in Listeria monocytogenes
Previous Article in Journal
A Pyrrhic Victory: The PMN Response to Ocular Bacterial Infections
Open AccessArticle

Mitochondria-Mediated Programmed Cell Death in Saccharomyces cerevisiae Induced by Betulinic Acid Is Accelerated by the Deletion of PEP4 Gene

Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Microorganisms 2019, 7(11), 538; https://doi.org/10.3390/microorganisms7110538
Received: 13 October 2019 / Revised: 27 October 2019 / Accepted: 5 November 2019 / Published: 7 November 2019
(This article belongs to the Section Microbial Biotechnology)
In this work, using Saccharomyces cerevisiae as a model, we showed that BetA could inhibit
cell proliferation and lead to lethal cytotoxicity accompanying programmed cell death (PCD).
Interestingly, it was found that vacuolar protease Pep4p played a pivotal role in BetA‐induced S.
cerevisiae PCD. The presence of Pep4p reduced the damage of BetA‐induced cells. This work implied
that BetA may induce cell death of S. cerevisiae through mitochondria‐mediated PCD, and the
deletion of Pep4 gene possibly accelerated the effect of PCD. The present investigation provided the
preliminary research for the complicated mechanism of BetA‐induced cell PCD regulated by vacular
protease Pep4p and lay the foundation for understanding of the Pep4p protein in an animal model.
Keywords: Saccharomyces cerevisiae; Pep4p; betulinic acid; mitochondria‐mediated programmed cell death Saccharomyces cerevisiae; Pep4p; betulinic acid; mitochondria‐mediated programmed cell death
MDPI and ACS Style

Lu, H.; Shu, Q.; Lou, H.; Chen, Q. Mitochondria-Mediated Programmed Cell Death in Saccharomyces cerevisiae Induced by Betulinic Acid Is Accelerated by the Deletion of PEP4 Gene. Microorganisms 2019, 7, 538.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop