Next Article in Journal / Special Issue
Recent Advances in Shiga Toxin-Producing Escherichia coli Research in Latin America
Previous Article in Journal
Gut Microbiota in Patients with Different Metabolic Statuses: Moscow Study
Previous Article in Special Issue
Variability in Characterizing Escherichia coli from Cattle Feces: A Cautionary Tale
Article Menu

Export Article

Open AccessBrief Report
Microorganisms 2018, 6(4), 99; https://doi.org/10.3390/microorganisms6040099

Antimicrobial Resistance in Class 1 Integron-Positive Shiga Toxin-Producing Escherichia coli Isolated from Cattle, Pigs, Food and Farm Environment

1
Laboratorio de Inmunoquímica y Biotecnología, Facultad de Ciencias Veterinarias, UNCPBA 7000, Argentina
2
Centro de Investigación Veterinaria de Tandil (CIVETAN), CONICET, CICPBA 7000, Argentina
3
Laboratorio de Resistencia Microbiana, Cátedra de Microbiología, Facultad de Farmacia y Bioquímica, UBA, Buenos Aires 1426, Argentina
4
Department of Medical Microbiology, University Medical Center Groningen, University of Groningen, 9713 GZ Groningen, The Netherlands. j.w.a.rossen@rug.nl (J.W.A.R.)
*
Author to whom correspondence should be addressed.
Received: 18 August 2018 / Revised: 24 September 2018 / Accepted: 26 September 2018 / Published: 28 September 2018
(This article belongs to the Special Issue Pathogenesis of Enterohaemorrhagic Escherichia coli)
Full-Text   |   PDF [243 KB, uploaded 28 September 2018]

Abstract

The aim of this study was to investigate the presence of class 1 integrons in a collection of Shiga toxin-producing Escherichia coli (STEC) from different origins and to characterize pheno- and genotypically the antimicrobial resistance associated to them. A collection of 649 isolates were screened for the class 1 integrase gene (intI1) by Polymerase chain reaction The variable region of class 1 integrons was amplified and sequenced. Positive strains were evaluated for the presence of antimicrobial resistance genes with microarray and for antimicrobial susceptibility by the disk diffusion method. Seven out of 649 STEC strains some to serogroups, O26, O103 and O130 isolated from cattle, chicken burger, farm environment and pigs were identified as positive for intl1. Different arrangements of gene cassettes were detected in the variable region of class 1 integron: dfrA16, aadA23 and dfrA1-aadA1. In almost all strains, phenotypic resistance to streptomycin, tetracycline, trimethoprim/sulfamethoxazole, and sulfisoxazole was observed. Microarray analyses showed that most of the isolates carried four or more antimicrobial resistance markers and STEC strains were categorized as Multridrug-resistant. Although antimicrobials are not usually used in the treatment of STEC infections, the presence of Multridrug-resistant in isolates collected from farm and food represents a risk for animal and human health. View Full-Text
Keywords: antimicrobial resistance; class 1 integron; STEC; reservoirs antimicrobial resistance; class 1 integron; STEC; reservoirs
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Colello, R.; Krüger, A.; Conza, J.D.; Rossen, J.W.A.; Friedrich, A.W.; Gutkind, G.; Etcheverría, A.I.; Padola, N.L. Antimicrobial Resistance in Class 1 Integron-Positive Shiga Toxin-Producing Escherichia coli Isolated from Cattle, Pigs, Food and Farm Environment. Microorganisms 2018, 6, 99.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Microorganisms EISSN 2076-2607 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top