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Microorganisms
Volume 13
Issue 8
10.3390/microorganisms13081932
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Article

Physico-Chemical and Antimicrobial Evaluation of Ozonated Olive Oil Produced with a Medical-Grade Generator for Veterinary Purposes

by
Călin Cosmin Repciuc
1,*,
Giulia-Ana-Maria Vișan
1,
Bernadette-Emoke Teleky
2,
Adela Pintea
3,
Cristiana Ștefania Novac
4 and
Nicușor Valentin Oros
5
1
Department of Surgery Anesthesiology and Intensive Care, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, Romania
2
Institute of Life Sciences, Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, Calea Mănăștur 3–5, 400372 Cluj-Napoca, Romania
3
Department of Biochemistry, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, Romania
4
Department of Microbiology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, Romania
5
Department of Internal Medicine, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, Romania
*
Author to whom correspondence should be addressed.
Microorganisms 2025, 13(8), 1932; https://doi.org/10.3390/microorganisms13081932
Submission received: 7 July 2025 / Revised: 11 August 2025 / Accepted: 14 August 2025 / Published: 18 August 2025
(This article belongs to the Special Issue Research on Antimicrobial Resistance and New Therapeutic Approaches)
Versions Notes

Abstract

The search for broad-spectrum antimicrobial products that do not generate resistance upon multiple applications has led to increased scientific and clinical interest in ozonated oils. The aim of this preliminary study was to evaluate the physico-chemical structure and antimicrobial properties of 1–12 h ozonated extra virgin olive oil produced in a veterinary clinic with a medical-grade generator. Prolonging the ozonation time causes a decrease in the iodine index, followed by significant increases in viscosity, acidity index, and peroxide values (p < 0.001). Other similar studies using industrial generators obtained satisfactory clinical results at peroxide values between 335 and 3590 mEq O2/Kg. Contrary to these established minimum thresholds, we found that ozonated olive oil with a peroxide index of 184 and 224 mEq O2/Kg exhibits fungicidal and bactericidal effects, demonstrating significant differences (p < 0.05) between tested and control samples for strains such as Staphylococcus aureus, E. faecalis, and E. coli. The 12 h ozonated oil showed itself to be efficient in the treatment of a 3-year-old cat presenting a chronic infected wound. The results encourage more detailed investigations of the antimicrobial effect of ozonated oils obtained with medical-grade generators and their evaluation on bacterial strains isolated from different individuals, followed by clinical evaluations and standardization.
Keywords: ozonated oil; peroxide; antibacterial; antifungal; wounds ozonated oil; peroxide; antibacterial; antifungal; wounds

Share and Cite

MDPI and ACS Style

Repciuc, C.C.; Vișan, G.-A.-M.; Teleky, B.-E.; Pintea, A.; Novac, C.Ș.; Oros, N.V. Physico-Chemical and Antimicrobial Evaluation of Ozonated Olive Oil Produced with a Medical-Grade Generator for Veterinary Purposes. Microorganisms 2025, 13, 1932. https://doi.org/10.3390/microorganisms13081932

AMA Style

Repciuc CC, Vișan G-A-M, Teleky B-E, Pintea A, Novac CȘ, Oros NV. Physico-Chemical and Antimicrobial Evaluation of Ozonated Olive Oil Produced with a Medical-Grade Generator for Veterinary Purposes. Microorganisms. 2025; 13(8):1932. https://doi.org/10.3390/microorganisms13081932

Chicago/Turabian Style

Repciuc, Călin Cosmin, Giulia-Ana-Maria Vișan, Bernadette-Emoke Teleky, Adela Pintea, Cristiana Ștefania Novac, and Nicușor Valentin Oros. 2025. "Physico-Chemical and Antimicrobial Evaluation of Ozonated Olive Oil Produced with a Medical-Grade Generator for Veterinary Purposes" Microorganisms 13, no. 8: 1932. https://doi.org/10.3390/microorganisms13081932

APA Style

Repciuc, C. C., Vișan, G.-A.-M., Teleky, B.-E., Pintea, A., Novac, C. Ș., & Oros, N. V. (2025). Physico-Chemical and Antimicrobial Evaluation of Ozonated Olive Oil Produced with a Medical-Grade Generator for Veterinary Purposes. Microorganisms, 13(8), 1932. https://doi.org/10.3390/microorganisms13081932

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