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Microorganisms
Volume 13
Issue 12
10.3390/microorganisms13122780
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

LuxR-Type Regulator RRP6 Positively Regulates the Biosynthesis of Plantaricin EF and Improves Its Production in Lactiplantibacillus plantarum 163

by
Yaxuan Liu
,
Siqi Liu
,
Zixian Li
,
Chuangen Huo
,
Guangli Wang
,
Xin Zeng
,
Bingyue Xin
and
Deyin Zhao
*
School of Life Science, Huaibei Normal University, Huaibei 235000, China
*
Author to whom correspondence should be addressed.
Microorganisms 2025, 13(12), 2780; https://doi.org/10.3390/microorganisms13122780 (registering DOI)
Submission received: 6 November 2025 / Revised: 28 November 2025 / Accepted: 4 December 2025 / Published: 6 December 2025
(This article belongs to the Special Issue Advances in Food Microbial Biotechnology)
Versions Notes

Abstract

The two-component system HPK6/RRP6 related to the pln locus of plantaricin biosynthesis was screened out. The overexpression of LuxR-type regulator RRP6 promoted the transcription of ABC transporter-related genes, thereby increasing plantaricin EF yield. Its yield in 163(rrp6) reached 16.01 mg/L, which was 1.20-fold that of the original strain. The regulatory mechanism indicated that RRP6 could bind to two sites of the plnG1 promoter, promoting its transcription and translation, accelerating the secretion of plantaricin and auto-inducing peptide, and enhancing the extracellular plantaricin yield. Amino acids Q73, R144, T171, and Y175 play a crucial role in the binding of RRP6. Furthermore, potential regulatory compensation within the Lactiplantibacillus plantarum 163 genome may compensate for the negative effects after the deletion of rrp6. These results provide a novel strategy for increasing plantaricin EF yield, which facilitates its large-scale application as a natural and safe food preservative in agriculture and the food industry.
Keywords: HPK6/RRP6; regulatory mechanism; plantaricin EF; improve production HPK6/RRP6; regulatory mechanism; plantaricin EF; improve production

Share and Cite

MDPI and ACS Style

Liu, Y.; Liu, S.; Li, Z.; Huo, C.; Wang, G.; Zeng, X.; Xin, B.; Zhao, D. LuxR-Type Regulator RRP6 Positively Regulates the Biosynthesis of Plantaricin EF and Improves Its Production in Lactiplantibacillus plantarum 163. Microorganisms 2025, 13, 2780. https://doi.org/10.3390/microorganisms13122780

AMA Style

Liu Y, Liu S, Li Z, Huo C, Wang G, Zeng X, Xin B, Zhao D. LuxR-Type Regulator RRP6 Positively Regulates the Biosynthesis of Plantaricin EF and Improves Its Production in Lactiplantibacillus plantarum 163. Microorganisms. 2025; 13(12):2780. https://doi.org/10.3390/microorganisms13122780

Chicago/Turabian Style

Liu, Yaxuan, Siqi Liu, Zixian Li, Chuangen Huo, Guangli Wang, Xin Zeng, Bingyue Xin, and Deyin Zhao. 2025. "LuxR-Type Regulator RRP6 Positively Regulates the Biosynthesis of Plantaricin EF and Improves Its Production in Lactiplantibacillus plantarum 163" Microorganisms 13, no. 12: 2780. https://doi.org/10.3390/microorganisms13122780

APA Style

Liu, Y., Liu, S., Li, Z., Huo, C., Wang, G., Zeng, X., Xin, B., & Zhao, D. (2025). LuxR-Type Regulator RRP6 Positively Regulates the Biosynthesis of Plantaricin EF and Improves Its Production in Lactiplantibacillus plantarum 163. Microorganisms, 13(12), 2780. https://doi.org/10.3390/microorganisms13122780

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