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Volume 12
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10.3390/microorganisms12030526
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Article

Platelet-Derived Microvesicles Contribute to the Pathophysiogenesis of Human Cutaneous Leishmaniasis: A Nano-Flow Cytometric Approach in Plasma Samples from Patients before and under Antimonial Treatment

by
Vanessa Fernandes de Abreu Costa
1,2,†,
Thaize Quiroga Chometon
1,2,‡,
Katherine Kelda Gomes de Castro
2,
Melissa Silva Gonçalves Ponte
1,2,
Maria Inês Fernandes Pimentel
3,
Marcelo Rosandiski Lyra
3 and
Alvaro Luiz Bertho
1,2,*
1
Laboratory of Immunoparasitology, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro 21040-360, RJ, Brazil
2
Flow Cytometry Core Facility, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro 21040-360, RJ, Brazil
3
Laboratory of Clinical Research and Surveillance in Leishmaniasis, Evandro Chagas National Institute of Infectious Diseases, FIOCRUZ, Rio de Janeiro 21040-360, RJ, Brazil
*
Author to whom correspondence should be addressed.
†
Current address: Beth Israel Deaconess Medical Center, Boston, MA 02215, USA.
‡
Current address: Flow Cytometry Core Facility, School of Biological Sciences, Faculty of Science, University of Auckland, Auckland 1142, New Zealand.
Microorganisms 2024, 12(3), 526; https://doi.org/10.3390/microorganisms12030526
Submission received: 4 December 2023 / Revised: 13 January 2024 / Accepted: 16 January 2024 / Published: 6 March 2024
(This article belongs to the Special Issue Leishmaniasis: Clinical Features and Epidemiology)
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Abstract

Cutaneous leishmaniasis is a neglected tropical disease caused, in Brazil, mainly by Leishmania braziliensis, which is a protozoan transmitted during the blood feeding of infected female sandflies. To control leishmaniasis, the participation of CD4+ Th1 cells together with macrophages, neutrophils, and other peripheral blood cells, including platelets, is necessary. These anuclear fragments, when activated, produce microvesicles (MVs) that can reach locations outside the blood, carrying molecules responsible for activating pro-inflammatory responses and antigen presentation. Using flow cytometry, this current study evaluated the frequency and concentration of platelet-derived MVs (pMVs) in plasma samples obtained from patients in the acute phase and undergoing treatment, as well as from healthy volunteers. Our results revealed a higher frequency and concentration of pMVs in the plasma of patients with acute CL when compared to all other groups studied. These results highlight the impact of pMVs in modulating the immune response of CL patients, correlating their higher concentrations and frequencies in CL-patient plasmas, with the acute inflammatory status of the disease and their reduction with beneficial results of systemic treatment with antimony. This knowledge is essential to define potential treatment protocols, as well as highlight pMVs as biomarkers for the different clinical stages of CL.
Keywords: human cutaneous leishmaniasis; platelet-derived microvesicles; extracellular vesicles; flow cytometry; plasma; antimony treatment human cutaneous leishmaniasis; platelet-derived microvesicles; extracellular vesicles; flow cytometry; plasma; antimony treatment

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MDPI and ACS Style

Costa, V.F.d.A.; Chometon, T.Q.; de Castro, K.K.G.; Ponte, M.S.G.; Pimentel, M.I.F.; Lyra, M.R.; Bertho, A.L. Platelet-Derived Microvesicles Contribute to the Pathophysiogenesis of Human Cutaneous Leishmaniasis: A Nano-Flow Cytometric Approach in Plasma Samples from Patients before and under Antimonial Treatment. Microorganisms 2024, 12, 526. https://doi.org/10.3390/microorganisms12030526

AMA Style

Costa VFdA, Chometon TQ, de Castro KKG, Ponte MSG, Pimentel MIF, Lyra MR, Bertho AL. Platelet-Derived Microvesicles Contribute to the Pathophysiogenesis of Human Cutaneous Leishmaniasis: A Nano-Flow Cytometric Approach in Plasma Samples from Patients before and under Antimonial Treatment. Microorganisms. 2024; 12(3):526. https://doi.org/10.3390/microorganisms12030526

Chicago/Turabian Style

Costa, Vanessa Fernandes de Abreu, Thaize Quiroga Chometon, Katherine Kelda Gomes de Castro, Melissa Silva Gonçalves Ponte, Maria Inês Fernandes Pimentel, Marcelo Rosandiski Lyra, and Alvaro Luiz Bertho. 2024. "Platelet-Derived Microvesicles Contribute to the Pathophysiogenesis of Human Cutaneous Leishmaniasis: A Nano-Flow Cytometric Approach in Plasma Samples from Patients before and under Antimonial Treatment" Microorganisms 12, no. 3: 526. https://doi.org/10.3390/microorganisms12030526

APA Style

Costa, V. F. d. A., Chometon, T. Q., de Castro, K. K. G., Ponte, M. S. G., Pimentel, M. I. F., Lyra, M. R., & Bertho, A. L. (2024). Platelet-Derived Microvesicles Contribute to the Pathophysiogenesis of Human Cutaneous Leishmaniasis: A Nano-Flow Cytometric Approach in Plasma Samples from Patients before and under Antimonial Treatment. Microorganisms, 12(3), 526. https://doi.org/10.3390/microorganisms12030526

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