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Microorganisms
Volume 12
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10.3390/microorganisms12112155
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Article

Mimicking Urinary Tract Infections Caused by Uropathogenic Escherichia coli Using a Human Three-Dimensional Tissue Engineering Model

by
Félix-Antoine Pellerin
1,
Élodie Dufresne
1,
Stéphane Chabaud
1,
Hazem Orabi
1,2 and
Stéphane Bolduc
1,3,*
1
Centre de Recherche en Organogénèse Expérimentale/LOEX, Regenerative Medicine Division, CHU de Québec-Université Laval Research Center, Quebec, QC G1J 1Z4, Canada
2
Department of Urology, Assiut University, Assiut 71515, Egypt
3
Department of Surgery, Faculty of Medicine, Laval University, Quebec, QC G1V 0A6, Canada
*
Author to whom correspondence should be addressed.
Microorganisms 2024, 12(11), 2155; https://doi.org/10.3390/microorganisms12112155
Submission received: 11 September 2024 / Revised: 17 October 2024 / Accepted: 18 October 2024 / Published: 26 October 2024
(This article belongs to the Section Medical Microbiology)
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Abstract

Uropathogenic Escherichia coli are the main causal agent of urinary tract infections. These diseases can affect more than half of women during their lifetime. Moreover, recurrent urinary tract infections can affect up to 30% of patients, leading to higher social and economic costs for the community. No efficient treatment against the recurrent form of the disease has been discovered. Due to the low average rate of successful translation from 2D cell culture and in vivo animal models into clinical trials, new models that mimic pathologies, such as those produced by tissue engineering, are needed. A model of human-derived 3D bladder mucosa was produced by tissue engineering techniques using collagen gels and organ-specific primary human stromal and epithelial cell populations. This model was used to mimic the different steps of a urinary tract infection: adhesion, invasion, intracellular bacterial community and quiescent intracellular reservoir formation and, finally, bacteria resurgence after umbrella cell exfoliation through chitosan exposure to mimic the recurrent infection. The uropathogenic strain UTI-89-GFP was used as infectious bacteria and BL-21-GFP strain as a control. Our model is unique and is the first step toward mimicking the different phases of a UTI in a human context.
Keywords: urinary tract infection; Escherichia coli; tissue engineering; urothelium; chitosan urinary tract infection; Escherichia coli; tissue engineering; urothelium; chitosan

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MDPI and ACS Style

Pellerin, F.-A.; Dufresne, É.; Chabaud, S.; Orabi, H.; Bolduc, S. Mimicking Urinary Tract Infections Caused by Uropathogenic Escherichia coli Using a Human Three-Dimensional Tissue Engineering Model. Microorganisms 2024, 12, 2155. https://doi.org/10.3390/microorganisms12112155

AMA Style

Pellerin F-A, Dufresne É, Chabaud S, Orabi H, Bolduc S. Mimicking Urinary Tract Infections Caused by Uropathogenic Escherichia coli Using a Human Three-Dimensional Tissue Engineering Model. Microorganisms. 2024; 12(11):2155. https://doi.org/10.3390/microorganisms12112155

Chicago/Turabian Style

Pellerin, Félix-Antoine, Élodie Dufresne, Stéphane Chabaud, Hazem Orabi, and Stéphane Bolduc. 2024. "Mimicking Urinary Tract Infections Caused by Uropathogenic Escherichia coli Using a Human Three-Dimensional Tissue Engineering Model" Microorganisms 12, no. 11: 2155. https://doi.org/10.3390/microorganisms12112155

APA Style

Pellerin, F.-A., Dufresne, É., Chabaud, S., Orabi, H., & Bolduc, S. (2024). Mimicking Urinary Tract Infections Caused by Uropathogenic Escherichia coli Using a Human Three-Dimensional Tissue Engineering Model. Microorganisms, 12(11), 2155. https://doi.org/10.3390/microorganisms12112155

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