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Microorganisms
Volume 12
Issue 1
10.3390/microorganisms12010183
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Communication

Application of Fourier Transform Infrared Spectroscopy to Discriminate Two Closely Related Bacterial Species: Bacillus anthracis and Bacillus cereus Sensu Stricto

by
Viviana Manzulli
1,*,
Miriam Cordovana
2,
Luigina Serrecchia
1,
Valeria Rondinone
1,
Lorenzo Pace
1,
Donatella Farina
1,
Dora Cipolletta
1,
Marta Caruso
1,
Rosa Fraccalvieri
1,
Laura Maria Difato
1,
Francesco Tolve
1,
Valerio Vetritto
1 and
Domenico Galante
1
1
Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 71121 Foggia, Italy
2
Bruker Daltonics GmbH, 28359 Bremen, Germany
*
Author to whom correspondence should be addressed.
Microorganisms 2024, 12(1), 183; https://doi.org/10.3390/microorganisms12010183
Submission received: 27 November 2023 / Revised: 29 December 2023 / Accepted: 12 January 2024 / Published: 17 January 2024
(This article belongs to the Special Issue New Methods in Microbial Research 3.0)
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Abstract

Fourier transform infrared spectroscopy (FTIRS) is a diagnostic technique historically used in the microbiological field for the characterization of bacterial strains in relation to the specific composition of their lipid, protein, and polysaccharide components. For each bacterial strain, it is possible to obtain a unique absorption spectrum that represents the fingerprint obtained based on the components of the outer cell membrane. In this study, FTIRS was applied for the first time as an experimental diagnostic tool for the discrimination of two pathogenic species belonging to the Bacillus cereus group, Bacillus anthracis and Bacillus cereus sensu stricto; these are two closely related species that are not so easy to differentiate using classical microbiological methods, representing an innovative technology in the field of animal health.
Keywords: FTIR-spectroscopy; IR Biotyper; Bacillus anthracis; Bacillus cereus sensu stricto FTIR-spectroscopy; IR Biotyper; Bacillus anthracis; Bacillus cereus sensu stricto

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MDPI and ACS Style

Manzulli, V.; Cordovana, M.; Serrecchia, L.; Rondinone, V.; Pace, L.; Farina, D.; Cipolletta, D.; Caruso, M.; Fraccalvieri, R.; Difato, L.M.; et al. Application of Fourier Transform Infrared Spectroscopy to Discriminate Two Closely Related Bacterial Species: Bacillus anthracis and Bacillus cereus Sensu Stricto. Microorganisms 2024, 12, 183. https://doi.org/10.3390/microorganisms12010183

AMA Style

Manzulli V, Cordovana M, Serrecchia L, Rondinone V, Pace L, Farina D, Cipolletta D, Caruso M, Fraccalvieri R, Difato LM, et al. Application of Fourier Transform Infrared Spectroscopy to Discriminate Two Closely Related Bacterial Species: Bacillus anthracis and Bacillus cereus Sensu Stricto. Microorganisms. 2024; 12(1):183. https://doi.org/10.3390/microorganisms12010183

Chicago/Turabian Style

Manzulli, Viviana, Miriam Cordovana, Luigina Serrecchia, Valeria Rondinone, Lorenzo Pace, Donatella Farina, Dora Cipolletta, Marta Caruso, Rosa Fraccalvieri, Laura Maria Difato, and et al. 2024. "Application of Fourier Transform Infrared Spectroscopy to Discriminate Two Closely Related Bacterial Species: Bacillus anthracis and Bacillus cereus Sensu Stricto" Microorganisms 12, no. 1: 183. https://doi.org/10.3390/microorganisms12010183

APA Style

Manzulli, V., Cordovana, M., Serrecchia, L., Rondinone, V., Pace, L., Farina, D., Cipolletta, D., Caruso, M., Fraccalvieri, R., Difato, L. M., Tolve, F., Vetritto, V., & Galante, D. (2024). Application of Fourier Transform Infrared Spectroscopy to Discriminate Two Closely Related Bacterial Species: Bacillus anthracis and Bacillus cereus Sensu Stricto. Microorganisms, 12(1), 183. https://doi.org/10.3390/microorganisms12010183

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