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Microorganisms
Volume 11
Issue 4
10.3390/microorganisms11041023
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Article

Easy, Flexible and Standardizable Anti-Nascent Biofilm Activity Assay to Assess Implant Materials

by
Jérome Vanheuverzwijn
1,
Eloise-Eliane Maillard
1,
Amal Mahat
1,
Lee Fowler
2,
Daniel Monteyne
3,
Leïla Bonnaud
4,
Nicolas Landercy
4,
Axel Hemberg
4,
Ana Janković
5,
Franck Meyer
1,
Vesna Mišković-Stanković
6,
Milena Stevanović
5,
Codruta Mirica
7,
David Pérez-Morga
3,
Reto Luginbuehl
8,9,
Christèle Combes
10,
Gabriel Furtos
11 and
Véronique Fontaine
1,*
1
Microbiology, Bioorganic and Macromolecular Chemistry Unit, Faculty of Pharmacy, Université libre de Bruxelles (ULB), Boulevard du Triomphe, 1050 Brussels, Belgium
2
Applied Materials Science, The Ångström Laboratory, Department of Engineering Sciences, Uppsala University, P.O. Box 534, 75121 Uppsala, Sweden
3
Laboratoire de Parasitologie Moléculaire, Faculté des Sciences & CMMI, Université Libre de Bruxelles (ULB), CP 300. Rue Prof. Jeener & Brachet, 12, 6041 Gosselies, Belgium
4
Center of Innovation and Research in Materials and Polymers, Materia Nova Research Center & University of Mons, 7000 Mons, Belgium
5
Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia
6
Faculty of Ecology and Environmental Protection, University Union-Nikola Tesla, Cara Dusana 62-64, 11158 Belgrade, Serbia
7
Department of Oral Health, Iuliu Hatieganu University of Medicine and Pharmacy, Victor Babes Street 15, 400012 Cluj-Napoca, Romania
8
Department of Biomedical Material Research, University of Bern, 3008 Bern, Switzerland
9
Blaser Swisslube, 3415 Hasle-Rüegsau, Switzerland
10
Centre Inter-Universitaire de Recherche et d’Ingénierie des Matériaux, CIRIMAT, Toulouse INP, Université Toulouse 3 Paul Sabatier, CNRS, Université de Toulouse, 4 allée Emile Monso, BP44362, CEDEX 4, 31030 Toulouse, France
11
Department of Dental Materials, Institute of Research in Chemistry, Babes-Bolyai University-Raluca Ripan, Fantanele Street 30, 400294 Cluj-Napoca, Romania
 
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*
Author to whom correspondence should be addressed.
Microorganisms 2023, 11(4), 1023; https://doi.org/10.3390/microorganisms11041023
Submission received: 6 March 2023 / Revised: 29 March 2023 / Accepted: 11 April 2023 / Published: 14 April 2023
(This article belongs to the Special Issue Biofilms and Device-Associated Infections)
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Abstract

Medical implants have improved the quality of life of many patients. However, surgical intervention may eventually lead to implant microbial contamination. The aims of this research were to develop an easy, robust, quantitative assay to assess surface antimicrobial activities, especially the anti-nascent biofilm activity, and to identify control surfaces, allowing for international comparisons. Using new antimicrobial assays to assess the inhibition of nascent biofilm during persistent contact or after transient contact with bacteria, we show that the 5 cent Euro coin or other metal-based antibacterial coins can be used as positive controls, as more than 4 log reduction on bacterial survival was observed when using either S. aureus or P. aeruginosa as targets. The methods and controls described here could be useful to develop an easy, flexible and standardizable assay to assess relevant antimicrobial activities of new implant materials developed by industries and academics.
Keywords: surface; anti-biofilm; infection; medical device; copper; metal ion surface; anti-biofilm; infection; medical device; copper; metal ion

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MDPI and ACS Style

Vanheuverzwijn, J.; Maillard, E.-E.; Mahat, A.; Fowler, L.; Monteyne, D.; Bonnaud, L.; Landercy, N.; Hemberg, A.; Janković, A.; Meyer, F.; et al. Easy, Flexible and Standardizable Anti-Nascent Biofilm Activity Assay to Assess Implant Materials. Microorganisms 2023, 11, 1023. https://doi.org/10.3390/microorganisms11041023

AMA Style

Vanheuverzwijn J, Maillard E-E, Mahat A, Fowler L, Monteyne D, Bonnaud L, Landercy N, Hemberg A, Janković A, Meyer F, et al. Easy, Flexible and Standardizable Anti-Nascent Biofilm Activity Assay to Assess Implant Materials. Microorganisms. 2023; 11(4):1023. https://doi.org/10.3390/microorganisms11041023

Chicago/Turabian Style

Vanheuverzwijn, Jérome, Eloise-Eliane Maillard, Amal Mahat, Lee Fowler, Daniel Monteyne, Leïla Bonnaud, Nicolas Landercy, Axel Hemberg, Ana Janković, Franck Meyer, and et al. 2023. "Easy, Flexible and Standardizable Anti-Nascent Biofilm Activity Assay to Assess Implant Materials" Microorganisms 11, no. 4: 1023. https://doi.org/10.3390/microorganisms11041023

APA Style

Vanheuverzwijn, J., Maillard, E.-E., Mahat, A., Fowler, L., Monteyne, D., Bonnaud, L., Landercy, N., Hemberg, A., Janković, A., Meyer, F., Mišković-Stanković, V., Stevanović, M., Mirica, C., Pérez-Morga, D., Luginbuehl, R., Combes, C., Furtos, G., & Fontaine, V. (2023). Easy, Flexible and Standardizable Anti-Nascent Biofilm Activity Assay to Assess Implant Materials. Microorganisms, 11(4), 1023. https://doi.org/10.3390/microorganisms11041023

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