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Microorganisms
Volume 11
Issue 3
10.3390/microorganisms11030617
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Article

Investigating Catheter-Related Infections in Southern Benin Hospitals: Identification, Susceptibility, and Resistance Genes of Involved Bacterial Strains

by
Victorien Tamègnon Dougnon
*,
Kevin Sintondji
,
Charles Hornel Koudokpon
,
Morènikè Houéto
,
Alidehou Jerrold Agbankpé
,
Phénix Assogba
,
Alida Oussou
,
Anderson Gnamy
,
Boris Legba
,
Abdoulaye Idrissou
and
Honoré Sourou Bankole
Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou BP 526, Benin
*
Author to whom correspondence should be addressed.
Microorganisms 2023, 11(3), 617; https://doi.org/10.3390/microorganisms11030617
Submission received: 21 January 2023 / Revised: 7 February 2023 / Accepted: 10 February 2023 / Published: 28 February 2023
(This article belongs to the Section Medical Microbiology)
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Abstract

The use of catheters and bladder catheters in hospitals can increase the risk of bacterial infections. This study aimed to identify the bacterial strains involved in catheter-related infections (CRI) in southern Benin hospitals. The study included 407 samples, including 95 catheter tip samples and 312 urine samples collected from bladder catheters from patients on the first day and 48 h after admission. The catheter tip samples were analyzed using traditional bacterial isolation and identification methods, while the urine samples were analyzed using VITEK-2. Antibiotic sensitivity was tested using the Kirby Bauer method, and virulence and resistance genes were detected through standard PCR. The results showed a predominance of Escherichia coli (53.5%), Klebsiella pneumoniae (23.3%), and Enterobacter aerogenes (7.0%) among Gram-negative bacilli, and coagulase-negative Staphylococcus as the most identified cocci. Bacterial susceptibility to antibiotics showed variable levels of resistance, with blaTEM being detected in 42.9% of identified bacterial species, followed by blaSHV (26.2%) and blaCTX-M-15 (16.7%). The blaNDM gene was only found in three identified bacterial strains, while vanA and vanB genes were detected in 3.2% of strains with a prevalence of 55% for the mecA gene. A prevalence of 18.8% for fimH was noted for the virulence genes. In conclusion, this study highlights the importance of following proper hygiene and aseptic practices during catheterization to effectively prevent CRIs. These findings should be used to improve interventions in hospitals and reduce healthcare-associated infections in developing countries.
Keywords: catheter-related infections; venous catheterization; bladder catheterization; antibiotic resistance genes; bacterial virulence genes; southern Benin catheter-related infections; venous catheterization; bladder catheterization; antibiotic resistance genes; bacterial virulence genes; southern Benin

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MDPI and ACS Style

Dougnon, V.T.; Sintondji, K.; Koudokpon, C.H.; Houéto, M.; Agbankpé, A.J.; Assogba, P.; Oussou, A.; Gnamy, A.; Legba, B.; Idrissou, A.; et al. Investigating Catheter-Related Infections in Southern Benin Hospitals: Identification, Susceptibility, and Resistance Genes of Involved Bacterial Strains. Microorganisms 2023, 11, 617. https://doi.org/10.3390/microorganisms11030617

AMA Style

Dougnon VT, Sintondji K, Koudokpon CH, Houéto M, Agbankpé AJ, Assogba P, Oussou A, Gnamy A, Legba B, Idrissou A, et al. Investigating Catheter-Related Infections in Southern Benin Hospitals: Identification, Susceptibility, and Resistance Genes of Involved Bacterial Strains. Microorganisms. 2023; 11(3):617. https://doi.org/10.3390/microorganisms11030617

Chicago/Turabian Style

Dougnon, Victorien Tamègnon, Kevin Sintondji, Charles Hornel Koudokpon, Morènikè Houéto, Alidehou Jerrold Agbankpé, Phénix Assogba, Alida Oussou, Anderson Gnamy, Boris Legba, Abdoulaye Idrissou, and et al. 2023. "Investigating Catheter-Related Infections in Southern Benin Hospitals: Identification, Susceptibility, and Resistance Genes of Involved Bacterial Strains" Microorganisms 11, no. 3: 617. https://doi.org/10.3390/microorganisms11030617

APA Style

Dougnon, V. T., Sintondji, K., Koudokpon, C. H., Houéto, M., Agbankpé, A. J., Assogba, P., Oussou, A., Gnamy, A., Legba, B., Idrissou, A., & Bankole, H. S. (2023). Investigating Catheter-Related Infections in Southern Benin Hospitals: Identification, Susceptibility, and Resistance Genes of Involved Bacterial Strains. Microorganisms, 11(3), 617. https://doi.org/10.3390/microorganisms11030617

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