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Article

Antimicrobial Resistance and Antimicrobial Activity of Staphylococcus lugdunensis Obtained from Two Spanish Hospitals

by
Rosa Fernández-Fernández
1,
Carmen Lozano
1,*,
Laura Ruiz-Ripa
1,
Beatriz Robredo
2,
José Manuel Azcona-Gutiérrez
3,
Carla Andrea Alonso
3,
Carmen Aspiroz
4,
Myriam Zarazaga
1 and
Carmen Torres
1,*
1
Área Bioquímica y Biología Molecular, OneHealth-UR Research Group, Universidad de La Rioja, 26006 Logroño, Spain
2
Área Didáctica de las Ciencias Experimentales, OneHealth-UR Research Group, Universidad de La Rioja, 26006 Logroño, Spain
3
Servicio de Microbiología, Hospital San Pedro, 26006 Logroño, Spain
4
Servicio de Microbiología, Hospital Royo Villanova, 50015 Zaragoza, Spain
*
Authors to whom correspondence should be addressed.
Microorganisms 2022, 10(8), 1480; https://doi.org/10.3390/microorganisms10081480
Submission received: 22 June 2022 / Revised: 14 July 2022 / Accepted: 19 July 2022 / Published: 22 July 2022
(This article belongs to the Special Issue Nosocomial Pathogens and Antibiotic Resistance)
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Abstract

Staphylococcus lugdunensis is a coagulase-negative-staphylococci (CoNS) that lately has gained special attention in public health as a human pathogen and also as a bacteriocin-producer bacteria. In this study, we characterized 56 S. lugdunensis isolates recovered from human samples in two Spanish hospitals. Antimicrobial susceptibility testing was performed and antimicrobial resistance and virulence genotypes were determined. Antimicrobial activity (AA) production was evaluated by the spot-on-lawn method against 37 indicator bacteria, including multidrug-resistant (MDR) isolates, and the presence of the lugD gene coding for lugdunin bacteriocin was analyzed by PCR. The antibiotic resistance detected was as follows (% resistance/genes detected): penicillin (44.6%/blaZ), oxacillin (1.8%/mecA on SCCmec-V), erythromycin-clindamycin inducible (7.1%/erm(C), msrA), tetracycline (5.3%/tetK), gentamicin and/or tobramycin (3.6%/ant(4′)-Ia, acc(6′)-aph(2″)), and fosfomycin (21.4%). A MDR phenotype was detected in 5% of isolates. Twenty-one of the S. lugdunensis isolates showed susceptibility to all 20 antibiotics tested (37.5%). The screening for AA revealed 23 antimicrobial producer (AP) isolates with relevant inhibition against coagulase-positive-staphylococci (CoPS), including both methicillin-susceptible and –resistant S. aureus. The lugD gene was detected in 84% of the 56 S. lugdunensis isolates. All of the AP S. lugdunensis isolates (n = 23) carried the lugD gene and it was also detected in 24 of the non-AP isolates, suggesting different gene expression levels. One of the AP isolates stood out due to its high antimicrobial activity against more than 70% of the indicator bacteria tested, so it will be further characterized at genomic and proteomic level.
Keywords: S. lugdunensis; coagulase-negative-staphylococci; antibiotic resistance; bacteriocins S. lugdunensis; coagulase-negative-staphylococci; antibiotic resistance; bacteriocins

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MDPI and ACS Style

Fernández-Fernández, R.; Lozano, C.; Ruiz-Ripa, L.; Robredo, B.; Azcona-Gutiérrez, J.M.; Alonso, C.A.; Aspiroz, C.; Zarazaga, M.; Torres, C. Antimicrobial Resistance and Antimicrobial Activity of Staphylococcus lugdunensis Obtained from Two Spanish Hospitals. Microorganisms 2022, 10, 1480. https://doi.org/10.3390/microorganisms10081480

AMA Style

Fernández-Fernández R, Lozano C, Ruiz-Ripa L, Robredo B, Azcona-Gutiérrez JM, Alonso CA, Aspiroz C, Zarazaga M, Torres C. Antimicrobial Resistance and Antimicrobial Activity of Staphylococcus lugdunensis Obtained from Two Spanish Hospitals. Microorganisms. 2022; 10(8):1480. https://doi.org/10.3390/microorganisms10081480

Chicago/Turabian Style

Fernández-Fernández, Rosa, Carmen Lozano, Laura Ruiz-Ripa, Beatriz Robredo, José Manuel Azcona-Gutiérrez, Carla Andrea Alonso, Carmen Aspiroz, Myriam Zarazaga, and Carmen Torres. 2022. "Antimicrobial Resistance and Antimicrobial Activity of Staphylococcus lugdunensis Obtained from Two Spanish Hospitals" Microorganisms 10, no. 8: 1480. https://doi.org/10.3390/microorganisms10081480

APA Style

Fernández-Fernández, R., Lozano, C., Ruiz-Ripa, L., Robredo, B., Azcona-Gutiérrez, J. M., Alonso, C. A., Aspiroz, C., Zarazaga, M., & Torres, C. (2022). Antimicrobial Resistance and Antimicrobial Activity of Staphylococcus lugdunensis Obtained from Two Spanish Hospitals. Microorganisms, 10(8), 1480. https://doi.org/10.3390/microorganisms10081480

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