Next Article in Journal
Vivax Malaria and the Potential Role of the Subtelomeric Multigene vir Superfamily
Next Article in Special Issue
Clinical Escherichia coli: From Biofilm Formation to New Antibiofilm Strategies
Previous Article in Journal
DNA Markers for Detection and Genotyping of Xanthomonas euroxanthea
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Microorganisms
Volume 10
Issue 6
10.3390/microorganisms10061082
microorganisms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Inhibition of In Vitro Clostridioides difficile Biofilm Formation by the Probiotic Yeast Saccharomyces boulardii CNCM I-745 through Modification of the Extracellular Matrix Composition

by
Pierre-Alexandre Lacotte
1,†,
Alexis Simons
1,2,†,
Sylvie Bouttier
1,
Jeanne Malet-Villemagne
1,
Valérie Nicolas
3 and
Claire Janoir
1,*
1
INRAE, Université Paris-Saclay, AgroParisTech, Micalis Institute, 92290 Châtenay-Malabry, France
2
Laboratoire Eau, Environnement et Systèmes Urbains (Leesu), Université Paris-Est Créteil, École des Ponts ParisTech, 94010 Créteil, France
3
Ingénierie et Plateformes au Service de l’Innovation (IPSIT), UMS IPSIT Université Paris-Saclay-US 31 INSERM-UAR 3679 CNRS, Plateforme d’Imagerie Cellulaire MIPSIT, 92290 Châtenay-Malabry, France
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to the work.
Microorganisms 2022, 10(6), 1082; https://doi.org/10.3390/microorganisms10061082
Submission received: 28 April 2022 / Revised: 21 May 2022 / Accepted: 21 May 2022 / Published: 24 May 2022
(This article belongs to the Special Issue Feature Papers in Microbial Biofilm Formation)
Browse Figures
Versions Notes

Abstract

Clostridioides difficile is responsible for post-antibiotic diarrhea and most of the pseudomembranous colitis cases. Multiple recurrences, one of the major challenges faced in C. difficile infection (CDI) management, can be considered as chronic infections, and the role of biofilm formation in CDI recurrences is now widely considered. Therefore, we explored if the probiotic yeast Saccharomyces boulardii CNCM I-745 could impact the in vitro formation of C. difficile biofilm. Biomass staining and viable bacterial cell quantification showed that live S. boulardii exerts an antagonistic effect on the biofilm formation for the three C. difficile strains tested. Confocal laser scanning microscopy observation revealed a weakening and an average thickness reduction of the biofilm structure when C. difficile is co-incubated with S. boulardii, compared to the single-species bacterial biofilm structure. These effects, that were not detected with another genetically close yeast, S. cerevisiae, seemed to require direct contact between the probiotic yeast and the bacterium. Quantification of the extrapolymeric matrix components, as well as results obtained after DNase treatment, revealed a significant decrease of eDNA, an essential structural component of the C. difficile biofilm matrix, in the dual-species biofilm. This modification could explain the reduced cohesion and robustness of C. difficile biofilms formed in the presence of S. boulardii CNCM I-745 and be involved in S. boulardii clinical preventive effect against CDI recurrences.
Keywords: Clostridioides difficile; biofilm; Saccharomyces boulardii CNCM I-745; matrix composition; eDNA Clostridioides difficile; biofilm; Saccharomyces boulardii CNCM I-745; matrix composition; eDNA

Share and Cite

MDPI and ACS Style

Lacotte, P.-A.; Simons, A.; Bouttier, S.; Malet-Villemagne, J.; Nicolas, V.; Janoir, C. Inhibition of In Vitro Clostridioides difficile Biofilm Formation by the Probiotic Yeast Saccharomyces boulardii CNCM I-745 through Modification of the Extracellular Matrix Composition. Microorganisms 2022, 10, 1082. https://doi.org/10.3390/microorganisms10061082

AMA Style

Lacotte P-A, Simons A, Bouttier S, Malet-Villemagne J, Nicolas V, Janoir C. Inhibition of In Vitro Clostridioides difficile Biofilm Formation by the Probiotic Yeast Saccharomyces boulardii CNCM I-745 through Modification of the Extracellular Matrix Composition. Microorganisms. 2022; 10(6):1082. https://doi.org/10.3390/microorganisms10061082

Chicago/Turabian Style

Lacotte, Pierre-Alexandre, Alexis Simons, Sylvie Bouttier, Jeanne Malet-Villemagne, Valérie Nicolas, and Claire Janoir. 2022. "Inhibition of In Vitro Clostridioides difficile Biofilm Formation by the Probiotic Yeast Saccharomyces boulardii CNCM I-745 through Modification of the Extracellular Matrix Composition" Microorganisms 10, no. 6: 1082. https://doi.org/10.3390/microorganisms10061082

APA Style

Lacotte, P.-A., Simons, A., Bouttier, S., Malet-Villemagne, J., Nicolas, V., & Janoir, C. (2022). Inhibition of In Vitro Clostridioides difficile Biofilm Formation by the Probiotic Yeast Saccharomyces boulardii CNCM I-745 through Modification of the Extracellular Matrix Composition. Microorganisms, 10(6), 1082. https://doi.org/10.3390/microorganisms10061082

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop