Review Reports

Round 1

Reviewer 1 Report

The manuscript entitled “ Application of a High-throughput Targeted Sequence AmpliSeq Procedure to Assess the Presence and Variants of Virulence Genes in Salmonella” written by Gao et al. contains scientific information that can be published after a minor revision.

The authors developed an amplicon based sequencing to detect and analyze the virulence gene of Salmonella for assessing the pathogenicity potential.

The manuscript required spelling correction and changes in the singular verb and plural verb correction in many parts.

For example,

Line 77: interprete the clinical -> interpret the clinical

Line 120: a large numbers of oligonucleotide -> “large numbers of oligonucleotide” or “a large number of oligonucleotide”

Line 273; frequency and location listed in Table S3 -> frequency and location are listed in Table S3.

Author Response

We agree with Reviewer 1:

Line 77: changed to "interpret"

Line 120" changed to "a large number of....."

Line 273: changed to "frequency and location are listed......"

 

Thank you.

Reviewer 2 Report

This study presents interesting new insights about the pathogenecity of Salmonella and provides a new tool a targeted, amplicon-based next-generation sequencing method (AmpliSeqSalm227VG) for the characterization of Salmonella virulence genes. The paper is sound and the conclusions are based on sufficient data. All necessary information is given.

Author Response

Thank you to Reviewer 2.

No changes requested by reviewer.