Molecular Profiles of Multiple Antimalarial Drug Resistance Markers in Plasmodium falciparum and Plasmodium vivax in the Mandalay Region, Myanmar
Abstract
:1. Introduction
2. Materials and Methods
2.1. Blood Samples
2.2. Amplification of Antimalarial Drug Resistance Genes
2.3. Sequence Polymorphism Analysis
2.4. Statistical Analysis
3. Results
3.1. Molecular Profiles of Antimalarial Drug Resistance Genes in Myanmar P. falciparum
3.1.1. Molecular Profiles of pfdhfr and pfdhps
3.1.2. Molecular Profiles of pfmdr-1 and pfcrt
3.1.3. Molecular Profiles of pfk13, pfubp-1, and pfcytb
3.1.4. Summary of Mutations in Multiple Antimalarial Drug Resistance Genes
3.2. Molecular Profiles of Antimalarial Drug Resistance Genes in Myanmar P. vivax
3.2.1. Molecular Profiles of pvdhfr and pvdhps
3.2.2. Molecular Profiles of pvmdr-1 and pvk12
3.2.3. Summary of Mutations in Multiple Antimalarial Drug Resistance Genes
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene Combination | Mutations | Naung Cho (n = 38) | Mandalay (n = 17) | Pyin Oo Lwin (n = 6) | Tha Beik Kyin (n = 19) |
pfdhfr + pfdhps | N51I/C59R/S108N + A437G | 0 | 0 | 0 | 0 |
N51I/C59R/S108N + A437G/K540E | 1 (2.6%) | 2 (11.8%) | 0 | 0 | |
N51I/C59R/S108N + A437G/K540E/A581G | 8 (21.1%) | 1 (5.9%) | 0 | 0 | |
Gene combination | Mutations | Naung Cho (n = 16) | Mandalay (n = 11) | Pyin Oo Lwin (n = 0) | Tha Beik Kyin (n = 6) |
pfmdr-1 + pfcrt | N86Y + K76T | 1 (6.3%) | 0 | 0 | 0 |
Gene Combination | Mutations | Naung Cho (n = 43) | Mandalay (n = 9) | Tha Beik Kyin (n = 12) |
---|---|---|---|---|
pvdhfr + pvdhps | F57I/S58R/T61M/S117T + S382A/A383G/A553G | 1 (2.3%) | 0 | 0 |
F57I/S58R/T61M/S117T + A383G/A553G | 19 (41.1%) | 5 (55.6%) | 1 (8.3%) | |
F57L/S58R/T61M/S117T + A383G/A553G | 7 (16.3%) | 2 (22.2%) | 5 (41.7%) | |
F57I/S58R/T61M/S117T + S382A/A383G | 0 | 0 | 0 | |
F57L/S58R/T61M/S117T + S382A/A383G | 0 | 1 (11.1%) | 0 | |
F57L/S58R/T61M/S117T + A383G | 2 (4.7%) | 0 | 1 (8.3%) | |
F57I/S58R/T61M/S117T + A383G | 1 (2.3%) | 0 | 0 | |
S58R/S117N + A383G/A553G | 6 (13.9%) | 0 | 1 (8.3%) | |
S58R/S117T + S382A/A383G/A553G | 0 | 0 | 1 (8.3%) | |
S58R/S117N + S382A/A553G | 1 (2.3%) | 0 | 0 | |
T61M/S117N + A383G/A553G | 1 (2.3%) | 0 | 0 | |
S58R/S117T + A383G/A553G | 0 | 1 (11.1%) | 0 | |
S117N + A383G/A553G | 3 (7.0%) | 0 | 0 | |
S58R/S117N + A553G | 0 | 0 | 2 (16.7%) |
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Lê, H.G.; Naw, H.; Kang, J.-M.; Võ, T.C.; Myint, M.K.; Htun, Z.T.; Lee, J.; Yoo, W.G.; Kim, T.-S.; Shin, H.-J.; et al. Molecular Profiles of Multiple Antimalarial Drug Resistance Markers in Plasmodium falciparum and Plasmodium vivax in the Mandalay Region, Myanmar. Microorganisms 2022, 10, 2021. https://doi.org/10.3390/microorganisms10102021
Lê HG, Naw H, Kang J-M, Võ TC, Myint MK, Htun ZT, Lee J, Yoo WG, Kim T-S, Shin H-J, et al. Molecular Profiles of Multiple Antimalarial Drug Resistance Markers in Plasmodium falciparum and Plasmodium vivax in the Mandalay Region, Myanmar. Microorganisms. 2022; 10(10):2021. https://doi.org/10.3390/microorganisms10102021
Chicago/Turabian StyleLê, Hương Giang, Haung Naw, Jung-Mi Kang, Tuấn Cường Võ, Moe Kyaw Myint, Zaw Than Htun, Jinyoung Lee, Won Gi Yoo, Tong-Soo Kim, Ho-Joon Shin, and et al. 2022. "Molecular Profiles of Multiple Antimalarial Drug Resistance Markers in Plasmodium falciparum and Plasmodium vivax in the Mandalay Region, Myanmar" Microorganisms 10, no. 10: 2021. https://doi.org/10.3390/microorganisms10102021