Screening of Circulation of Usutu and West Nile Viruses: A One Health Approach in Humans, Domestic Animals and Mosquitoes in Burkina Faso, West Africa
Abstract
:1. Introduction
2. Materials and Methods
2.1. Samples
2.1.1. Blood Donors
2.1.2. Sera Specimens from Fever of Unknown Etiologies
2.1.3. Sera Samples from Animals
- -
- A total of 81 horse sera samples (50 from Ouagadougou and 31 from Bobo-Dioulasso) were collected. The average age of the 81 horses analyzed was 7.11 years, with extremes ranging from 10 months to 27 years, corresponding to 79.01% of males and 20.99% of females. The samples were taken in the breeding sites by a veterinary technician. No horse was vaccinated against WNV, and all showed no symptoms at the time of sampling.
- -
- A total of 52 dogs (25 from Ouagadougou and 27 from Bobo-Dioulasso) were included in our study. All samples were taken at the owners’ homes, and no dog showed symptoms. The average age of the dogs was 2.77 years, with extremes ranging from 2 months to 12 years. There were 34 males and 18 females.
- -
- A total of 50 sera samples from chickens from traditional farms were collected in Ouagadougou (25) and Bobo-Dioulasso (25) and analyzed. The average age was 1.3 years, with extremes ranging from 5 months to 3 years. There were 10 males and 40 females. All samples were collected from poultry markets.
- -
- A total of 21 domestic pigeons from traditional breeding were included in the study. We collected 10 sera samples in Ouagadougou and 11 in Bobo-Dioulasso. The average age of the pigeons was 12.8 months, with extremes ranging from 1 month to 2 years. There were 9 males and 12 females. All samples were collected from owners’ homes.
2.1.4. Mosquito Samples
2.1.5. Mosquito Sorting and Crushing
2.2. Competitive Enzyme-Linked Immunosorbent Assay
2.3. Seroneutralization Assays
2.4. USUV and WNV qRT-PCR
2.5. Statistical Analysis
2.6. Ethical Statement and Informed Consent
3. Results
3.1. WNV and USUV Infections in Humans Samples
3.2. WNV and USUV Infections in Domestic Animals
3.3. WNV and USUV Screening in Mosquitoes
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Variable | cELISA Positive n (%) | MNT Positive n (%) | ||
---|---|---|---|---|
USUV+ | WNV+ | USUV + WNV+ | ||
Total | 389 (77.64) | 71 * (14.17) | 96 * (19.16) | 52 * (10.38) |
Origin | ||||
Ouagadougou | 216 (84.37) | 44 (17.18) | 50 (19.53) | 31 (12.10) |
Bobo-Dioulasso | 173 (70.61) | 27 (11.02) | 46 (18.77) | 21 (8.57) |
Gender | ||||
Male | 302 (78.03) | 56 (14.47) | 82 (21.18) | 45 (11.62) |
Female | 87 (76.31) | 15 (13.15) | 14 (12.28) | 7 (6.14) |
Age | ||||
18–24 | 130 (76.47) | 24 (14.11) | 33 (19.41) | 17 (10.0) |
25–34 | 146 (74.11) | 24 (12.18) | 30 (15.22) | 15 (7.65) |
35–44 | 81 (85.26) | 17 (17.89) | 23 (24.21) | 14 (14.73) |
45–59 | 32 (82.05) | 6 (15.38) | 10 (25.64) | 6 (15.38) |
(A) | |||
Variable | Positive n (%) | Odds Ratio IC95% | p-Value |
Origin | 0.0547 | ||
Ouagadougou | 44 (17.18) | 1 | |
Bobo-Dioulasso | 27 (11.02) | 0.6 [0.36–1] | |
Gender | 0.8786 | ||
Male | 56 (14.47) | 1 | |
Female | 15 (13.15) | 0.9 [0.49–1.66] | |
Age | 0.6213 | ||
18–24 | 24 (14.11) | 1 | |
25–34 | 24 (12.18) | 0.84 [0.46–1.54] | |
35–44 | 17 (17.89) | 1.33 [0.67–2.62] | |
45–59 | 6 (15.38) | 1.11 [0.42–2.93] | |
(B) | |||
Variable | Positive n (%) | Odds Ratio IC95% | p-Value |
Origin | 0.9097 | ||
Ouagadougou | 50 (19.53) | 1 | |
Bobo-Dioulasso | 46 (18.77) | 0.95 [0.61–1.49] | |
Gender | 0.0415 * | ||
Male | 82 (20.15) | 1 | |
Female | 14 (12.28) | 0.52 [0.28–0.96] | |
Age | 0.2040 | ||
18–24 | 31 (18.23) | 1 | |
25–34 | 29 (14.72) | 0.75 [0.44–1.29] | |
35–44 | 22 (23.15) | 1.33 [0.73–2.45] | |
45–59 | 10 (25.64) | 1.43 [0.63–3.22] |
Species | Number of Specimens | cELISA | MNT Antibodies Titer | Conclusion | |
---|---|---|---|---|---|
USUV | WNV | ||||
Horses | 81 | 73 | 128 | 128 | USUV + WNV+ |
32 | Negative | USUV+ | |||
32 | 256 | WNV+ | |||
64 | 64 | USUV + WNV+ | |||
8 | Negative | USUV+ | |||
8 | Negative | USUV+ | |||
32 | 128 | WNV+ | |||
Negative | 128 | WNV+ | |||
Negative | 128 | WNV+ | |||
Negative | 32 | WNV+ | |||
Negative | 32 | WNV+ | |||
Negative | 8 | WNV+ | |||
Negative | 256 | WNV+ | |||
Negative | 16 | WNV+ | |||
Negative | 32 | WNV+ | |||
Negative | 16 | WNV+ | |||
Negative | 16 | WNV+ | |||
Dogs | 52 | 14 | 0 | 64 | WNV+ |
Chickens | 50 | 4 | Negative | Negative | Negative |
Pigeons | 21 | 2 | 8 | Negative | USUV+ |
0 | 8 | WNV+ |
Species | Results | Total | Origin | |
---|---|---|---|---|
Ouagadougou | Bobo-Dioulasso | |||
Horses | USUV + n (%) | 5 (6.17%) | 2 (4%) | 3 (9.67%) |
WNV + n (%) | 14 (17.28%) | 1 (2%) | 13 (41.93) | |
Dogs | USUV + n (%) | 0 (00%) | 0 (00%) | 0 (00%) |
WNV + n (%) | 1 (1.92%) | 1 (4%) | 0 (00%) | |
Chickens | USUV + n (%) | 0 (00%) | 0 (00%) | 0 (00%) |
WNV + n (%) | 0 (00%) | 0 (00%) | 0 (00%) | |
Pigeons | USUV + n (%) | 1 (4.76%) | 0 (00%) | 1 (9.09%) |
WNV + n (%) | 1 (4.76%) | 0 (00%) | 1 (9.09%) |
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Tinto, B.; Kaboré, D.P.A.; Kagoné, T.S.; Constant, O.; Barthelemy, J.; Kiba-Koumaré, A.; Van de Perre, P.; Dabiré, R.K.; Baldet, T.; Gutierrez, S.; et al. Screening of Circulation of Usutu and West Nile Viruses: A One Health Approach in Humans, Domestic Animals and Mosquitoes in Burkina Faso, West Africa. Microorganisms 2022, 10, 2016. https://doi.org/10.3390/microorganisms10102016
Tinto B, Kaboré DPA, Kagoné TS, Constant O, Barthelemy J, Kiba-Koumaré A, Van de Perre P, Dabiré RK, Baldet T, Gutierrez S, et al. Screening of Circulation of Usutu and West Nile Viruses: A One Health Approach in Humans, Domestic Animals and Mosquitoes in Burkina Faso, West Africa. Microorganisms. 2022; 10(10):2016. https://doi.org/10.3390/microorganisms10102016
Chicago/Turabian StyleTinto, Bachirou, Didier Patinde Alexandre Kaboré, Thérèse Samdapawindé Kagoné, Orianne Constant, Jonathan Barthelemy, Alice Kiba-Koumaré, Philippe Van de Perre, Roch Kounbobr Dabiré, Thierry Baldet, Serafin Gutierrez, and et al. 2022. "Screening of Circulation of Usutu and West Nile Viruses: A One Health Approach in Humans, Domestic Animals and Mosquitoes in Burkina Faso, West Africa" Microorganisms 10, no. 10: 2016. https://doi.org/10.3390/microorganisms10102016