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Article

Comparison of Coxiella burnetii Excretion between Sheep and Goats Naturally Infected with One Cattle-Associated Genotype

1
Clinic for Swine and Small Ruminants, Forensic Medicine and Ambulatory Service, University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, 30173 Hannover, Germany
2
Lower Saxony State Office for Consumer Protection and Food Safety (LAVES), Food and Veterinary Institute Braunschweig/Hannover, Eintrachtweg 17, 30173 Hannover, Germany
3
Bundeswehr Institute of Microbiology, Neuherbergstraße 11, 80937 Munich, Germany
4
Department of Psychology, Faculty of Life Sciences, Humboldt-Universität zu Berlin, Unter den Linden 6, 10099 Berlin, Germany
5
Bundeswehr Medical Service Headquarters VI-2, Medical Intelligence & Information (MI2), Dachauer Straße 128, 80637 Munich, Germany
*
Author to whom correspondence should be addressed.
Pathogens 2020, 9(8), 652; https://doi.org/10.3390/pathogens9080652
Received: 14 July 2020 / Revised: 8 August 2020 / Accepted: 10 August 2020 / Published: 13 August 2020
(This article belongs to the Special Issue Q Fever)
The main reservoir of Coxiella (C.) burnetii are ruminants. They shed the pathogen through birth products, vaginal mucus, faeces and milk. A direct comparison of C. burnetii excretions between naturally infected sheep and goats was performed on the same farm to investigate species-specific differences. The animals were vaccinated with an inactivated C. burnetii phase I vaccine at the beginning of the study period for public health reasons. Vaginal and rectal swabs along with milk specimens were taken monthly during the lambing period and once again at the next lambing season. To estimate the environmental contamination of the animals’ housings, nasal swabs from every animal were taken simultaneously. Moreover, dust samples from the windowsills and straw beddings were collected. All samples were examined by qPCR targeting the IS1111 gene and the MLVA/VNTR typing method was performed. Whole genome sequencing was applied to determine the number of IS1111 copies followed by a calculation of C. burnetii genome equivalents of each sample. The cattle-associated genotype C7 was detected containing 29 IS1111 copies. Overall, goats seem to shed more C. burnetii through vaginal mucus and in particular shed more and for longer via the rectal route than sheep. This is supported by the larger quantities of C. burnetii DNA detected in caprine nasal swabs and environmental samples compared to the ovine ones. Transmission of C. burnetii from cattle to small ruminants must also be considered. View Full-Text
Keywords: Coxiella burnetii; sheep; goat; cattle; environmental contamination; nasal swabs; whole genome sequencing; MLVA/VNTR; Q fever; zoonosis Coxiella burnetii; sheep; goat; cattle; environmental contamination; nasal swabs; whole genome sequencing; MLVA/VNTR; Q fever; zoonosis
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MDPI and ACS Style

Bauer, B.; Prüfer, L.; Walter, M.; Ganter, I.; Frangoulidis, D.; Runge, M.; Ganter, M. Comparison of Coxiella burnetii Excretion between Sheep and Goats Naturally Infected with One Cattle-Associated Genotype. Pathogens 2020, 9, 652. https://doi.org/10.3390/pathogens9080652

AMA Style

Bauer B, Prüfer L, Walter M, Ganter I, Frangoulidis D, Runge M, Ganter M. Comparison of Coxiella burnetii Excretion between Sheep and Goats Naturally Infected with One Cattle-Associated Genotype. Pathogens. 2020; 9(8):652. https://doi.org/10.3390/pathogens9080652

Chicago/Turabian Style

Bauer, Benjamin, Louise Prüfer, Mathias Walter, Isabel Ganter, Dimitrios Frangoulidis, Martin Runge, and Martin Ganter. 2020. "Comparison of Coxiella burnetii Excretion between Sheep and Goats Naturally Infected with One Cattle-Associated Genotype" Pathogens 9, no. 8: 652. https://doi.org/10.3390/pathogens9080652

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