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Review

Contributions of Mass Spectrometry-Based Proteomics to Understanding Salmonella-Host Interactions

1
Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
2
Department of Microbiology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this article.
Pathogens 2020, 9(7), 581; https://doi.org/10.3390/pathogens9070581
Received: 22 June 2020 / Revised: 13 July 2020 / Accepted: 16 July 2020 / Published: 17 July 2020
(This article belongs to the Special Issue Salmonella Persistent Infections)
As a model pathogen, Salmonella invades both phagocytic and non-phagocytic host cells and adopts an intracellular lifestyle in a membrane-bound compartment during infection. Therefore, a systemic overview of Salmonella adaptations to distinct host cells together with host remodeling will assist us in charting the landscape of host-pathogen interactions. Central to the Salmonella-host interplay are bacterial virulence factors (effectors) that are injected into host cells by type III secretion systems (T3SSs). Despite great progress, functional studies of bacterial effectors have experienced daunting challenges as well. In the last decade, mass spectrometry-based proteomics has evolved into a powerful technological platform that can quantitatively measure thousands of proteins in terms of their expression as well as post-translational modifications. Here, we will review the applications of high-throughput proteomic technologies in understanding the dynamic reprogramming of both Salmonella and host proteomes during the course of infection. Furthermore, we will summarize the progress in utilizing affinity purification-mass spectrometry to screen for host substrates of Salmonella T3SS effectors. Finally, we will critically discuss some limitations/challenges with current proteomic platforms in the context of host-pathogen interactions and highlight some emerging technologies that may offer the promise of tackling these problems. View Full-Text
Keywords: mass spectrometry; proteomics; Salmonella; host-pathogen interactions; AP-MS mass spectrometry; proteomics; Salmonella; host-pathogen interactions; AP-MS
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MDPI and ACS Style

Zhang, B.; Liu, B.; Zhou, Y.; Zhang, X.; Zou, Q.; Liu, X. Contributions of Mass Spectrometry-Based Proteomics to Understanding Salmonella-Host Interactions. Pathogens 2020, 9, 581. https://doi.org/10.3390/pathogens9070581

AMA Style

Zhang B, Liu B, Zhou Y, Zhang X, Zou Q, Liu X. Contributions of Mass Spectrometry-Based Proteomics to Understanding Salmonella-Host Interactions. Pathogens. 2020; 9(7):581. https://doi.org/10.3390/pathogens9070581

Chicago/Turabian Style

Zhang, Buyu, Bohao Liu, Yinglin Zhou, Xinxiang Zhang, Qinghua Zou, and Xiaoyun Liu. 2020. "Contributions of Mass Spectrometry-Based Proteomics to Understanding Salmonella-Host Interactions" Pathogens 9, no. 7: 581. https://doi.org/10.3390/pathogens9070581

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