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Article

Virulence Characterization of Listeria monocytogenes, Listeria innocua, and Listeria welshimeri Isolated from Fish and Shrimp Using In Vivo Early Zebrafish Larvae Models and Molecular Study

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Department of Industrial and Food Microbiology, University of Warmia and Mazury, Plac Cieszyński 1, 10-726 Olsztyn, Poland
2
Department of Pathophysiology Forensic Veterinary Medicine and Administration, University of Warmia and Mazury, ul. Oczapowskiego 14, 10-719 Olsztyn, Poland
*
Author to whom correspondence should be addressed.
Pathogens 2020, 9(12), 1028; https://doi.org/10.3390/pathogens9121028
Received: 14 November 2020 / Revised: 4 December 2020 / Accepted: 6 December 2020 / Published: 8 December 2020
Listeriosis is one of the most notable foodborne diseases and is characterized by high rates of mortality. L. monocytogenes is the main cause of human listeriosis outbreaks, however, there are isolated cases of disease caused by other species of the genus Listeria. The aim of this study was to evaluate strains of L. monocytogenes (n = 7), L. innocua (n = 6), and L. welshimeri (n = 2) isolated from fish and shrimps for their virulence based on the presence of virulence genes and the in vivo Danio rerio (zebrafish) larvae models. A total of 15 strains were analyzed. The zebrafish larvae model showed that the larvae injected with L. monocytogenes strains were characterized by the lowest survival rate (46.5%), followed by L. innocua strains (64.2%) and L. welshimeri (83.0%) strains. Multiplex PCRs were used for detection of selected virulence genes (luxS, actA2, prfA, inlB, rrn, iap, sigB, plcB, actA, hlyA), the majority of which were present in L. monocytogenes. Only a few virulence-related genes were found in L. welshimeri, however, no correlation between the occurrence of these genes and larval survival was confirmed. This research highlights the importance of the potential impact that Listeria spp. strains isolated from fish and shrimps may have on consumers. View Full-Text
Keywords: Listeria spp.; Listeria monocytogenes; Listeria innocua; Listeria welshimeri; Danio rerio; pathogenicity Listeria spp.; Listeria monocytogenes; Listeria innocua; Listeria welshimeri; Danio rerio; pathogenicity
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MDPI and ACS Style

Zakrzewski, A.J.; Chajęcka-Wierzchowska, W.; Zadernowska, A.; Podlasz, P. Virulence Characterization of Listeria monocytogenes, Listeria innocua, and Listeria welshimeri Isolated from Fish and Shrimp Using In Vivo Early Zebrafish Larvae Models and Molecular Study. Pathogens 2020, 9, 1028. https://doi.org/10.3390/pathogens9121028

AMA Style

Zakrzewski AJ, Chajęcka-Wierzchowska W, Zadernowska A, Podlasz P. Virulence Characterization of Listeria monocytogenes, Listeria innocua, and Listeria welshimeri Isolated from Fish and Shrimp Using In Vivo Early Zebrafish Larvae Models and Molecular Study. Pathogens. 2020; 9(12):1028. https://doi.org/10.3390/pathogens9121028

Chicago/Turabian Style

Zakrzewski, Arkadiusz J., Wioleta Chajęcka-Wierzchowska, Anna Zadernowska, and Piotr Podlasz. 2020. "Virulence Characterization of Listeria monocytogenes, Listeria innocua, and Listeria welshimeri Isolated from Fish and Shrimp Using In Vivo Early Zebrafish Larvae Models and Molecular Study" Pathogens 9, no. 12: 1028. https://doi.org/10.3390/pathogens9121028

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