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Synergism of the Combination of Traditional Antibiotics and Novel Phenolic Compounds against Escherichia coli

1
Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency, Gimcheon-si 39660, Korea
2
Laboratory of Veterinary Pharmacokinetics and Pharmacodynamics, College of Veterinary Medicine, Kyungpook National University, Bukgu, Daegu 41566, Korea
3
Division of Vectors and Parasitic Diseases, Korea Centers for Diseases Control and Prevention, Cheongju 28159, Korea
*
Author to whom correspondence should be addressed.
Pathogens 2020, 9(10), 811; https://doi.org/10.3390/pathogens9100811
Received: 31 August 2020 / Revised: 30 September 2020 / Accepted: 1 October 2020 / Published: 3 October 2020
Pathogenic Escherichia coli (E. coli)-associated infections are becoming difficult to treat because of the rapid emergence of antibiotic-resistant strains. Novel approaches are required to prevent the progression of resistance and to extend the lifespan of existing antibiotics. This study was designed to improve the effectiveness of traditional antibiotics against E. coli using a combination of the gallic acid (GA), hamamelitannin, epicatechin gallate, epigallocatechin, and epicatechin. The fractional inhibitory concentration index (FICI) of each of the phenolic compound-antibiotic combinations against E. coli was ascertained. Considering the clinical significance and FICI, two combinations (hamamelitannin-erythromycin and GA-ampicillin) were evaluated for their impact on certain virulence factors of E. coli. Finally, the effects of hamamelitannin and GA on Rattus norvegicus (IEC-6) cell viability were investigated. The FICIs of the antibacterial combinations against E. coli were 0.281–1.008. The GA-ampicillin and hamamelitannin-erythromycin combinations more effectively prohibited the growth, biofilm viability, and swim and swarm motilities of E. coli than individual antibiotics. The concentration of hamamelitannin and GA required to reduce viability by 50% (IC50) in IEC-6 cells was 988.54 μM and 564.55 μM, correspondingly. GA-ampicillin and hamamelitannin-erythromycin may be potent combinations and promising candidates for eradicating pathogenic E. coli in humans and animals. View Full-Text
Keywords: synergistic effect; antibacterial agents; bacterial pathogenicity; ampicillin; erythromycin synergistic effect; antibacterial agents; bacterial pathogenicity; ampicillin; erythromycin
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    Doi: 10.5281/zenodo.4008777
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    Description: Effects of HAMA-ERY and GA-AMP combinations on the viability of Rattus norvegicus small intestine (IEC-6) cells
MDPI and ACS Style

Hossain, M.A.; Park, H.-C.; Park, S.-W.; Park, S.-C.; Seo, M.-G.; Her, M.; Kang, J. Synergism of the Combination of Traditional Antibiotics and Novel Phenolic Compounds against Escherichia coli. Pathogens 2020, 9, 811. https://doi.org/10.3390/pathogens9100811

AMA Style

Hossain MA, Park H-C, Park S-W, Park S-C, Seo M-G, Her M, Kang J. Synergism of the Combination of Traditional Antibiotics and Novel Phenolic Compounds against Escherichia coli. Pathogens. 2020; 9(10):811. https://doi.org/10.3390/pathogens9100811

Chicago/Turabian Style

Hossain, Md. A., Hae-Chul Park, Sung-Won Park, Seung-Chun Park, Min-Goo Seo, Moon Her, and JeongWoo Kang. 2020. "Synergism of the Combination of Traditional Antibiotics and Novel Phenolic Compounds against Escherichia coli" Pathogens 9, no. 10: 811. https://doi.org/10.3390/pathogens9100811

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