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Transcriptomic and Functional Studies of the RGS Protein Rax1 in Aspergillus fumigatus

Department of Microbiology, Graduate School, Daejeon University, Daejeon 34520, Korea
Soonchunhyang Institute of Medi-bio Science, Soonchunhyang University, Chungcheongnam-do 31151, Korea
Department of Microbiology, Institute for Applied Biosciences, Karlsruhe Institute of Technology, Fritz-Haber Weg 4, D-76131 Karlsruhe, Germany
Departments of Bacteriology and Genetics, University of Wisconsin-Madison, Madison, WI 53706, USA
Department of Systems Biotechnology, Konkuk University, Seoul 05029, Korea
Authors to whom correspondence should be addressed.
Contributed equally to this work.
Pathogens 2020, 9(1), 36;
Received: 6 December 2019 / Revised: 26 December 2019 / Accepted: 30 December 2019 / Published: 31 December 2019
(This article belongs to the Special Issue Signal Transduction in Aspergillus fumigatus)
In the comparative transcriptomic studies of wild type (WT) and rax1 null mutant strains, we obtained an average of 22,222,727 reads of 101 bp per sample and found that 183 genes showed greater than 2.0-fold differential expression, where 92 and 91 genes were up-and down-regulated in Δrax1 compared to WT, respectively. In accordance with the significantly reduced levels of gliM and casB transcripts in the absence of rax1, the Δrax1 mutant exhibited increased sensitivity to exogenous gliotoxin (GT) without affecting levels of GT production. Moreover, Δrax1 resulted in significantly restricted colony growth and reduced viability under endoplasmic reticulum stress condition. In summary, Rax1 positively affects expression of gliM and metacaspase genes. View Full-Text
Keywords: Aspergillus fumigatus; GliM; metacaspase; Rax1; RNA-seq Aspergillus fumigatus; GliM; metacaspase; Rax1; RNA-seq
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Choi, Y.-H.; Lee, M.-W.; Igbalajobi, O.A.; Yu, J.-H.; Shin, K.-S. Transcriptomic and Functional Studies of the RGS Protein Rax1 in Aspergillus fumigatus. Pathogens 2020, 9, 36.

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