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Open AccessCommunication

Inactivation of Zika Virus by Photoactive Iodonaphthyl Azide Preserves Immunogenic Potential of the Virus

Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD 20853, USA
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Author to whom correspondence should be addressed.
Pathogens 2019, 8(4), 188; https://doi.org/10.3390/pathogens8040188
Received: 16 August 2019 / Revised: 1 October 2019 / Accepted: 9 October 2019 / Published: 12 October 2019
(This article belongs to the Special Issue Current Advances in Flavivirus Research)
Zika virus’s (ZIKV) emergence as a pathogen of significant public health importance has accelerated efforts to develop a ZIKV vaccine. To date, the need for an effective ZIKV vaccine is unmet. In this study, we report inactivation of ZIKV using a hydrophobic photoactive compound: 1, 5 iodonaphthyl azide (INA). 50 and 100 µM of INA completely inactivated ZIKV (INA-ZIKV). Western blot and ELISA analysis show some loss of the binding capacity of INA-iZIKV to anti-ZIKV monoclonal antibodies; however, immunization of mice with INA-iZIKV demonstrated seroconversion and ZIKV-neutralizing antibody response. RNA isolated from INA-iZIKV did not induce productive infection in Vero cells, suggesting inactivation of ZIKV RNA. These results suggest that in the absence of an approved ZIKV vaccine, INA-iZIKV can be pursued as a viable ZIKV vaccine candidate.
Keywords: Zika virus; vaccine; inactivation; iodonaphthyl azide; immunization Zika virus; vaccine; inactivation; iodonaphthyl azide; immunization
MDPI and ACS Style

Austin, A.L.; Galasso, B.; Nickens, C.; Knollmann-Ritschel, B.; Sharma, A. Inactivation of Zika Virus by Photoactive Iodonaphthyl Azide Preserves Immunogenic Potential of the Virus. Pathogens 2019, 8, 188.

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