Genetic and Pathogenic Variability of Mycogone perniciosa Isolates Causing Wet Bubble Disease on Agaricus bisporus in China
by
Dan Li 1,2,†, Frederick Leo Sossah 1,2,†
, Yang Yang 1,2,†, Zhenghui Liu 1, Yueting Dai 1, Bing Song 1,2, Yongping Fu 1,2,* and Yu Li 1,2
Dan Li 1,2,†, Frederick Leo Sossah 1,2,†, Yang Yang 1,2,†, Zhenghui Liu 1, Yueting Dai 1, Bing Song 1,2, Yongping Fu 1,2,* and Yu Li 1,2
1
Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China
2
School of Plant Protection, Jilin Agricultural University, Changchun 130118, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Pathogens 2019, 8(4), 179; https://doi.org/10.3390/pathogens8040179
Received: 3 September 2019 / Revised: 24 September 2019 / Accepted: 1 October 2019 / Published: 8 October 2019
Wet bubble disease, caused by Mycogone perniciosa, is a major threat to Agaricus bisporus production in China. In order to understand the variability in genetic, pathogenicity, morphology, and symptom production of the fungus, 18 isolates of the pathogen were collected from diseased A. bisporus in different provinces in China. The isolates were characterized by a combination of morphological, cultural, molecular, and pathogenicity testing on different strains of A. bisporus and amplified fragment length polymorphism (AFLP) analysis. The 18 isolates were identified by Koch’s postulate and confirmed different pathogenic variability among them. The yellow to brown isolates were more virulent than the white isolates. AFLP markers clustered the isolates into two distinct groups based on their colony color, with a high level of polymorphism of Jaccard similarities range from 0.39% to 0.64%. However, there was no evidence of an association between the genetic diversity and the geographical origin of the isolates. Through knowledge of the genetic diversity, phenotypic virulence of M. perniciosa is a key factor for successful breeding of resistant strains of A. bisporus and developing of an integrated disease management strategy to manage wet bubble disease of A. bisporus.
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Keywords:
AFLP; Mycogone perniciosa; wet bubble disease; pathogenicity; diversity
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MDPI and ACS Style
Li, D.; Sossah, F.L.; Yang, Y.; Liu, Z.; Dai, Y.; Song, B.; Fu, Y.; Li, Y. Genetic and Pathogenic Variability of Mycogone perniciosa Isolates Causing Wet Bubble Disease on Agaricus bisporus in China. Pathogens 2019, 8, 179.
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