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Open AccessArticle

Recombinant Newcastle Disease Virus (NDV) Expressing Sigma C Protein of Avian Reovirus (ARV) Protects against Both ARV and NDV in Chickens

1
Recombinant DNA Lab, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243 122, India
2
Avian Diseases Section, Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243 122, India
3
Immunology Section, Indian Veterinary Research Institute, Izatnagar, Bareilly 243 122, India
4
Institute of Marine & Environmental Technology, University of Maryland Baltimore County, Baltimore, MD 21202, USA
*
Authors to whom correspondence should be addressed.
Pathogens 2019, 8(3), 145; https://doi.org/10.3390/pathogens8030145
Received: 31 July 2019 / Revised: 25 August 2019 / Accepted: 6 September 2019 / Published: 10 September 2019
(This article belongs to the Special Issue Newcastle Disease Vaccines: Current Research and Future Trends)
Newcastle disease (ND) and avian reovirus (ARV) infections are a serious threat to the poultry industry, which causes heavy economic losses. The mesogenic NDV strain R2B is commonly used as a booster vaccine in many Asian countries to control the disease. In this seminal work, a recombinant NDV strain R2B expressing the sigma C (σC) gene of ARV (rNDV-R2B-σC) was generated by reverse genetics, characterized in vitro and tested as a bivalent vaccine candidate in chickens. The recombinant rNDV-R2B-σC virus was attenuated as compared to the parent rNDV-R2B virus as revealed by standard pathogenicity assays. The generated vaccine candidate, rNDV-R2B-σC, could induce both humoral and cell mediated immune responses in birds and gave complete protection against virulent NDV and ARV challenges. Post-challenge virus shedding analysis revealed a drastic reduction in NDV shed, as compared to unvaccinated birds. View Full-Text
Keywords: newcastle disease virus; avian reovirus-σC protein; bivalent vaccine candidate; humoral and cell mediated immune responses; protection efficacy newcastle disease virus; avian reovirus-σC protein; bivalent vaccine candidate; humoral and cell mediated immune responses; protection efficacy
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Saikia, D.P.; Yadav, K.; Pathak, D.C.; Ramamurthy, N.; D’Silva, A.L.; Marriappan, A.K.; Ramakrishnan, S.; Vakharia, V.N.; Chellappa, M.M.; Dey, S. Recombinant Newcastle Disease Virus (NDV) Expressing Sigma C Protein of Avian Reovirus (ARV) Protects against Both ARV and NDV in Chickens. Pathogens 2019, 8, 145.

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