Next Article in Journal
HPV16 E6 and E7 Upregulate Interferon-Induced Antiviral Response Genes ISG15 and IFIT1 in Human Trophoblast Cells
Next Article in Special Issue
Microfluidics-Based Approaches to the Isolation of African Trypanosomes
Previous Article in Journal
A Review of Salmonella and Squamates (Lizards, Snakes and Amphisbians): Implications for Public Health
Previous Article in Special Issue
ProtozoaDB 2.0: A Trypanosoma Brucei Case Study
Article Menu

Export Article

Open AccessArticle
Pathogens 2017, 6(3), 39; https://doi.org/10.3390/pathogens6030039

The Lipid Raft Proteome of African Trypanosomes Contains Many Flagellar Proteins

1
Departments of Pathology and Microbiology-Immunology, Northwestern University, Chicago, IL 60611, USA
2
Department of Pathology and Laboratory Medicine, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA
Present address: Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115, USA.
*
Author to whom correspondence should be addressed.
Received: 10 August 2017 / Revised: 22 August 2017 / Accepted: 22 August 2017 / Published: 24 August 2017
(This article belongs to the Special Issue Trypanosoma brucei)
Full-Text   |   PDF [754 KB, uploaded 24 August 2017]   |  

Abstract

Lipid rafts are liquid-ordered membrane microdomains that form by preferential association of 3-β-hydroxysterols, sphingolipids and raft-associated proteins often having acyl modifications. We isolated lipid rafts of the protozoan parasite Trypanosoma brucei and determined the protein composition of lipid rafts in the cell. This analysis revealed a striking enrichment of flagellar proteins and several putative signaling proteins in the lipid raft proteome. Calpains and intraflagellar transport proteins, in particular, were found to be abundant in the lipid raft proteome. These findings provide additional evidence supporting the notion that the eukaryotic cilium/flagellum is a lipid raft-enriched specialized structure with high concentrations of sterols, sphingolipids and palmitoylated proteins involved in environmental sensing and cell signaling. View Full-Text
Keywords: trypanosomes; lipid rafts; flagellum; intraflagellar transport trypanosomes; lipid rafts; flagellum; intraflagellar transport
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Sharma, A.I.; Olson, C.L.; Engman, D.M. The Lipid Raft Proteome of African Trypanosomes Contains Many Flagellar Proteins. Pathogens 2017, 6, 39.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Pathogens EISSN 2076-0817 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top