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Pathogens 2017, 6(3), 39;

The Lipid Raft Proteome of African Trypanosomes Contains Many Flagellar Proteins

Departments of Pathology and Microbiology-Immunology, Northwestern University, Chicago, IL 60611, USA
Department of Pathology and Laboratory Medicine, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA
Present address: Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115, USA.
Author to whom correspondence should be addressed.
Received: 10 August 2017 / Revised: 22 August 2017 / Accepted: 22 August 2017 / Published: 24 August 2017
(This article belongs to the Special Issue Trypanosoma brucei)
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Lipid rafts are liquid-ordered membrane microdomains that form by preferential association of 3-β-hydroxysterols, sphingolipids and raft-associated proteins often having acyl modifications. We isolated lipid rafts of the protozoan parasite Trypanosoma brucei and determined the protein composition of lipid rafts in the cell. This analysis revealed a striking enrichment of flagellar proteins and several putative signaling proteins in the lipid raft proteome. Calpains and intraflagellar transport proteins, in particular, were found to be abundant in the lipid raft proteome. These findings provide additional evidence supporting the notion that the eukaryotic cilium/flagellum is a lipid raft-enriched specialized structure with high concentrations of sterols, sphingolipids and palmitoylated proteins involved in environmental sensing and cell signaling. View Full-Text
Keywords: trypanosomes; lipid rafts; flagellum; intraflagellar transport trypanosomes; lipid rafts; flagellum; intraflagellar transport

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Sharma, A.I.; Olson, C.L.; Engman, D.M. The Lipid Raft Proteome of African Trypanosomes Contains Many Flagellar Proteins. Pathogens 2017, 6, 39.

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