Comparison of Potato and Asian Citrus Psyllid Adult and Nymph Transcriptomes Identified Vector Transcripts with Potential Involvement in Circulative, Propagative Liberibacter Transmission
by
Tonja W. Fisher 1, Meenal Vyas 1, Ruifeng He 2, William Nelson 3, Joseph M. Cicero 1, Mark Willer 3, Ryan Kim 4, Robin Kramer 4, Greg A. May 4, John A. Crow 4, Carol A. Soderlund 3, David R. Gang 2 and Judith K. Brown 1,*
Tonja W. Fisher 1, Meenal Vyas 1, Ruifeng He 2, William Nelson 3, Joseph M. Cicero 1, Mark Willer 3, Ryan Kim 4, Robin Kramer 4, Greg A. May 4, John A. Crow 4, Carol A. Soderlund 3, David R. Gang 2 and Judith K. Brown 1,*
1
School of Plant Sciences, The University of Arizona, Tucson, AZ 85721, USA
2
Institute of Biological Chemistry, Washington State University, Pullman, WA 99164, USA
3
BIO5, The University of Arizona, Tucson, AZ 85721, USA
4
National Center for Genome Resources, 2935 Rodeo Park Drive East, Santa Fe, NM 87505, USA
*
Author to whom correspondence should be addressed.
Pathogens 2014, 3(4), 875-907; https://doi.org/10.3390/pathogens3040875
Received: 19 September 2014 / Revised: 18 October 2014 / Accepted: 20 October 2014 / Published: 3 November 2014
The potato psyllid (PoP) Bactericera cockerelli (Sulc) and Asian citrus psyllid (ACP) Diaphorina citri Kuwayama are the insect vectors of the fastidious plant pathogen, Candidatus Liberibacter solanacearum (CLso) and Ca. L. asiaticus (CLas), respectively. CLso causes Zebra chip disease of potato and vein-greening in solanaceous species, whereas, CLas causes citrus greening disease. The reliance on insecticides for vector management to reduce pathogen transmission has increased interest in alternative approaches, including RNA interference to abate expression of genes essential for psyllid-mediated Ca. Liberibacter transmission. To identify genes with significantly altered expression at different life stages and conditions of CLso/CLas infection, cDNA libraries were constructed for CLso-infected and -uninfected PoP adults and nymphal instars. Illumina sequencing produced 199,081,451 reads that were assembled into 82,224 unique transcripts. PoP and the analogous transcripts from ACP adult and nymphs reported elsewhere were annotated, organized into functional gene groups using the Gene Ontology classification system, and analyzed for differential in silico expression. Expression profiles revealed vector life stage differences and differential gene expression associated with Liberibacter infection of the psyllid host, including invasion, immune system modulation, nutrition, and development.
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Keywords:
circulative-propagative transmission; fastidious plant bacteria; psyllid vector; transcriptome
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MDPI and ACS Style
Fisher, T.W.; Vyas, M.; He, R.; Nelson, W.; Cicero, J.M.; Willer, M.; Kim, R.; Kramer, R.; May, G.A.; Crow, J.A.; Soderlund, C.A.; Gang, D.R.; Brown, J.K. Comparison of Potato and Asian Citrus Psyllid Adult and Nymph Transcriptomes Identified Vector Transcripts with Potential Involvement in Circulative, Propagative Liberibacter Transmission. Pathogens 2014, 3, 875-907.
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