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Article

Lecanicillium psalliotae (Hypocreales: Cordycipitaceae) Exerts Ovicidal and Larvicidal Effects against the Sheep Blood-Feeding Nematode Haemonchus contortus through Its Liquid Culture Filtrates

by
Gustavo Pérez-Anzúrez
1,2,
Pedro Mendoza-de Gives
1,*,
Miguel Ángel Alonso-Díaz
3,
Elke von Son-de Fernex
3,
Adolfo Paz-Silva
4,
María Eugenia López-Arellano
1 and
Agustín Olmedo-Juárez
1
1
Laboratory of Helminthology, National Centre for Disciplinary Research in Animal Health and Innocuity (CENID-SAI), National Institute for Research in Forestry, Agriculture and Livestock, INIFAP-SADER, Jiutepec 62550, Mexico
2
Production Sciences and Animal Health, Faculty of Veterinary Medicine and Zootechnics, National Autonomous University of Mexico, Coyoacán 04510, Mexico
3
Tropical Livestock Center, Faculty of Veterinary Medicine and Zootechnics, National Autonomous University of Mexico, Martínez de la Torre 93600, Mexico
4
Department of Animal Pathology, Faculty of Veterinary, University of Santiago de Compostela, 27142 Lugo, Spain
*
Author to whom correspondence should be addressed.
Pathogens 2024, 13(7), 588; https://doi.org/10.3390/pathogens13070588
Submission received: 15 May 2024 / Revised: 5 July 2024 / Accepted: 14 July 2024 / Published: 16 July 2024
(This article belongs to the Special Issue Prevalence, Pathology, and Alternative Control of Intestinal Parasites)
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Abstract

Nematophagous fungi (NF) form part of the soil microbiota and are natural enemies of nematodes, helping to regulate nematode populations. A verticillate NF isolated from soil from Tepalcingo, Mexico, was morphologically and molecularly characterised. This fungus was cultured in two different liquid media—Czapek-Dox broth (CzDoxB) and sweet potato dextrose broth (SPDB)—for 21 days. The ovicidal (OA) and larvicidal (LA) activities of fungal liquid culture filtrates (LCFs) were assessed in 96-well microtitre plates at different concentrations against Haemonchus contortus after 48 h. The morphological and molecular identification revealed the presence of Lecanicillium psalliotae. Additionally, the groups of compounds associated with nematocidal activity were determined from a qualitative chemical profile (QCP) using different reagents. The highest OA of the LCFs was obtained at 25 mg/mL from SPDB and CzDoxB and amounted to 97.2 and 99.06%, respectively. Meanwhile, the highest LA recorded with these LCFs at 100 mg/mL was 54.27% and 96.8%, respectively. The QCP revealed the presence of alkaloids and tannins in both LCFs that have previously been associated with nematocidal activity. Lecanicillium psalliotae exerted an important effect on H. contortus and could be of significance in future studies focused on the control and prevention of haemonchosis in small ruminants.
Keywords: Lecanicillium; nematophagous fungi; alkaloids; tannins; ovicidal; larvicidal Lecanicillium; nematophagous fungi; alkaloids; tannins; ovicidal; larvicidal

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MDPI and ACS Style

Pérez-Anzúrez, G.; Mendoza-de Gives, P.; Alonso-Díaz, M.Á.; von Son-de Fernex, E.; Paz-Silva, A.; López-Arellano, M.E.; Olmedo-Juárez, A. Lecanicillium psalliotae (Hypocreales: Cordycipitaceae) Exerts Ovicidal and Larvicidal Effects against the Sheep Blood-Feeding Nematode Haemonchus contortus through Its Liquid Culture Filtrates. Pathogens 2024, 13, 588. https://doi.org/10.3390/pathogens13070588

AMA Style

Pérez-Anzúrez G, Mendoza-de Gives P, Alonso-Díaz MÁ, von Son-de Fernex E, Paz-Silva A, López-Arellano ME, Olmedo-Juárez A. Lecanicillium psalliotae (Hypocreales: Cordycipitaceae) Exerts Ovicidal and Larvicidal Effects against the Sheep Blood-Feeding Nematode Haemonchus contortus through Its Liquid Culture Filtrates. Pathogens. 2024; 13(7):588. https://doi.org/10.3390/pathogens13070588

Chicago/Turabian Style

Pérez-Anzúrez, Gustavo, Pedro Mendoza-de Gives, Miguel Ángel Alonso-Díaz, Elke von Son-de Fernex, Adolfo Paz-Silva, María Eugenia López-Arellano, and Agustín Olmedo-Juárez. 2024. "Lecanicillium psalliotae (Hypocreales: Cordycipitaceae) Exerts Ovicidal and Larvicidal Effects against the Sheep Blood-Feeding Nematode Haemonchus contortus through Its Liquid Culture Filtrates" Pathogens 13, no. 7: 588. https://doi.org/10.3390/pathogens13070588

APA Style

Pérez-Anzúrez, G., Mendoza-de Gives, P., Alonso-Díaz, M. Á., von Son-de Fernex, E., Paz-Silva, A., López-Arellano, M. E., & Olmedo-Juárez, A. (2024). Lecanicillium psalliotae (Hypocreales: Cordycipitaceae) Exerts Ovicidal and Larvicidal Effects against the Sheep Blood-Feeding Nematode Haemonchus contortus through Its Liquid Culture Filtrates. Pathogens, 13(7), 588. https://doi.org/10.3390/pathogens13070588

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