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Volume 11
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10.3390/pathogens11050550
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Article

Immunogenicity Analysis of the Recombinant Plasmodium falciparum Surface-Related Antigen in Mice

by
Jia-Li Yu
2,†,
Qing-Yang Liu
1,†,
Bo Yang
2,†,
Yi-Fan Sun
2,
Ya-Ju Wang
3,
Jian Jiang
3,
Bo Wang
4,
Yang Cheng
2,* and
Qiu-Bo Wang
1,*
1
Department of Clinical Laboratory, Wuxi 9th Affiliated Hospital of Soochow University (Wuxi 9th People’s Hospital), Wuxi 214000, China
2
Laboratory of Pathogen Infection and Immunity, Department of Public Health and Preventive Medicine, Wuxi School of Medicine, Jiangnan University, Wuxi 214000, China
3
Wuxi Red Cross Blood Center, Wuxi 214000, China
4
Department of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University, Hefei 230000, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Pathogens 2022, 11(5), 550; https://doi.org/10.3390/pathogens11050550
Submission received: 24 February 2022 / Revised: 29 April 2022 / Accepted: 29 April 2022 / Published: 7 May 2022
(This article belongs to the Special Issue Mosquito-Borne Diseases: Novel Control Strategies)
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Abstract

Plasmodium falciparum, mainly distributed in tropical and subtropical regions of the world, has received widespread attention owing to its severity. As a novel protein, P. falciparum surface-related antigen (PfSRA) has the structural and functional characteristics to be considered as a malaria vaccine candidate; however, limited information is available on its immunogenicity. Here, we expressed three fragments of recombinant PfSRA in an Escherichia coli system and further analyzed its immunogenicity. The results showed that rPfSRA-immunized mice produced specific antibodies with high endpoint titers (1:10,000 to 1:5,120,000) and affinity antibodies (i.e., rPfSRA-F1a (97.70%), rPfSRA-F2a (69.62%), and rPfSRA-F3a (91.87%)). In addition, the sera of immunized mice recognized both the native PfSRA and recombinant PfSRA, the rPfSRA antibodies inhibited the invasion of P. falciparum into the erythrocytes, and they were dose-dependent in vitro. This study confirmed PfSRA could be immunogenic, especially the F1a at the conserved region N-terminal and provided further support for it as a vaccine candidate against P.falciparum.
Keywords: Plasmodium falciparum; surface-related antigen; immunogenicity; invasion inhibition Plasmodium falciparum; surface-related antigen; immunogenicity; invasion inhibition

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MDPI and ACS Style

Yu, J.-L.; Liu, Q.-Y.; Yang, B.; Sun, Y.-F.; Wang, Y.-J.; Jiang, J.; Wang, B.; Cheng, Y.; Wang, Q.-B. Immunogenicity Analysis of the Recombinant Plasmodium falciparum Surface-Related Antigen in Mice. Pathogens 2022, 11, 550. https://doi.org/10.3390/pathogens11050550

AMA Style

Yu J-L, Liu Q-Y, Yang B, Sun Y-F, Wang Y-J, Jiang J, Wang B, Cheng Y, Wang Q-B. Immunogenicity Analysis of the Recombinant Plasmodium falciparum Surface-Related Antigen in Mice. Pathogens. 2022; 11(5):550. https://doi.org/10.3390/pathogens11050550

Chicago/Turabian Style

Yu, Jia-Li, Qing-Yang Liu, Bo Yang, Yi-Fan Sun, Ya-Ju Wang, Jian Jiang, Bo Wang, Yang Cheng, and Qiu-Bo Wang. 2022. "Immunogenicity Analysis of the Recombinant Plasmodium falciparum Surface-Related Antigen in Mice" Pathogens 11, no. 5: 550. https://doi.org/10.3390/pathogens11050550

APA Style

Yu, J.-L., Liu, Q.-Y., Yang, B., Sun, Y.-F., Wang, Y.-J., Jiang, J., Wang, B., Cheng, Y., & Wang, Q.-B. (2022). Immunogenicity Analysis of the Recombinant Plasmodium falciparum Surface-Related Antigen in Mice. Pathogens, 11(5), 550. https://doi.org/10.3390/pathogens11050550

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