Next Article in Journal
Evaluation of Serological Methods and a New Real-Time Nested PCR for Small Ruminant Lentiviruses
Next Article in Special Issue
A Three-Year Look at the Phylogenetic Profile, Antimicrobial Resistance, and Associated Virulence Genes of Uropathogenic Escherichia coli
Previous Article in Journal
New and Confirmed Foci of Tick-Borne Encephalitis Virus (TBEV) in Northern Germany Determined by TBEV Detection in Ticks
Previous Article in Special Issue
Distinct Characteristics of Escherichia coli Isolated from Patients with Urinary Tract Infections in a Medical Center at a Ten-Year Interval
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Pathogens
Volume 11
Issue 2
10.3390/pathogens11020127
pathogens-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Virulence Profiles and Antibiotic Susceptibility of Escherichia coli Strains from Pet Reptiles

by
Marta Dec
1,*,
Dagmara Stepien-Pysniak
1,
Klaudiusz Szczepaniak
2,
Barbara Turchi
3 and
Renata Urban-Chmiel
1
1
Department of Veterinary Prevention and Avian Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-033 Lublin, Poland
2
Department of Parasitology and Fish Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-033 Lublin, Poland
3
Department of Veterinary Science, University of Pisa, Viale delle Piagge 2, 56124 Pisa, Italy
*
Author to whom correspondence should be addressed.
Pathogens 2022, 11(2), 127; https://doi.org/10.3390/pathogens11020127
Submission received: 10 November 2021 / Revised: 6 January 2022 / Accepted: 19 January 2022 / Published: 21 January 2022
(This article belongs to the Special Issue Virulence and Antimicrobial Resistance of Emergent Pathogens)
Browse Figures
Versions Notes

Abstract

:
Exotic reptiles are increasingly being bred as pets in many countries around the world, including Poland. However, the close contact between reptiles and their owners provides favourable conditions for the transmission of zoonotic pathogens. In this work, we examined E. coli isolates from 67 captive reptiles regarding their virulence, antibiotic susceptibility, phylogenetic affiliation, and genetic diversity. The incidence of E. coli was highest in snakes (51.6%, 16 isolates/31 samples), and slightly lower in turtles (44.4%, 8/18) and lizards (44.4%, 8/18). Genes encoding virulence factors were confirmed in 50% of isolates and the most common were the traT (37.5%, n = 12), fyuA (21.87%, n = 7), and irp-2 (15.62%, n = 5). The majority (71.87%, n = 23) of E. coli isolates were susceptible to all of the antimicrobial substances used in the study. Streptomycin resistance (21.87%, n = 7) was the most frequent, while resistance to other antimicrobial substances was sporadic. One strain (3.12%) was classified as multidrug-resistant. The presence of resistance genes (aadA, tetA, tetB, tetM, and blaTEM) was confirmed in 12.5% (n = 4) of the isolates. The majority (65.6%, n = 21) of E. coli isolates represented the B1 phylogenetic group. (GTG)5-PCR fingerprinting showed considerable genetic variation in the pool of tested isolates. The frequency of E. coli in reptiles is much lower than in mammals or birds. Due to the presence of virulence genes, characteristic of both intestinal pathogenic E. coli (IPEC) and extraintestinal pathogenic E. coli (ExPEC), reptilian strains of E. coli have pathogenic potential, and therefore people in contact with these animals should follow good hygiene practices.

1. Introduction

In the last few years, exotic reptiles have risen in popularity as pets, with a population of over 9 million in European households. The estimated number of captive reptiles in Poland is 215,000 [1] (pp. 44, 50). The corn snake, ball python, steppe tortoise, Greek tortoise, and lizards such as the bearded agama, chameleon and geckos are very popular [2]. Pet reptiles are sometimes kept in terrariums, but often they are free to move about in homes and treated as companion animals. In addition, reptile exhibitions organized in public spaces provide opportunities not only to observe these animals, but also to touch or hold them. However, close contact between reptiles and humans poses a public health risk, as these animals may harbour and excrete potentially pathogenic microorganisms. Reptiles are well recognized as asymptomatic carriers of Salmonella, including serotypes which can cause infections in humans, known as reptile-associated salmonellosis (RAS). At the same time, data on the characteristics of reptilian E. coli strains (RepEC), which, similarly to Salmonella, belong to the Enterobacteriaceae family, are very limited [3,4].
Within the E. coli species, apart from commensals commonly colonizing the intestines of mammals and birds, there are also intestinal pathogenic E. coli (IPEC) and extraintestinal pathogenic E. coli (ExPEC) strains. The formers are diarrhoeagenic pathogens, and the latter colonize other parts of the host’s body but can also exist as commensals. Each of these two groups contains a number of pathotypes that differ in their range of virulence factors and the type of disease they cause. However, E. coli has high genome plasticity, and hybrid strains carrying a combination of both IPEC and ExPEC virulence-associated genes are known as well [5]. ExpPEC strains, i.e., avian pathogenic E. coli (APEC), uropathogenic E. coli (UPEC), and neonatal meningitis E. coli (NMEC), are responsible for many diseases of humans and animals, such as urinary tract infections (UTIs), pneumonia, meningitis, diverse intra-abdominal infections, soft tissue infections, osteomyelitis, and sepsis [6]. Due to the high genetic similarity of virulent ExpPEC strains from animals and humans, it has been suggested that livestock or pets may be a reservoir for the transmission of E. coli infections [7,8]. Among IPECs, the enterohaemorrhagic E. coli (EHEC) strains that produce Shiga toxin are considered the most dangerous for humans. The symptoms of EHEC infection are bloody diarrhoea and severe colitis, and some patients develop haemolytic uraemic syndrome (HUS), which can be fatal [9]. The main reservoirs of Shiga-toxin-producing strains of E. coli are ruminants (cattle, sheep, and goats), but these strains have also been detected in reptiles. Moreover, lizards can be carriers of other IPECs as well, i.e., enteroaggregative E. coli (EAEC), enterotoxigenic E. coli (ETEC), and enteropathogenic E. coli (EPEC) [9,10].
Bearing in mind the above, as well as the growing interest in reptile breeding and the lack of information on the occurrence and characteristics of RepEC, in this study we aimed to determine the prevalence of E. coli strains in captive reptiles and their virulence and antibiotic sensitivity profiles, as well as their phylogenetic affiliation and genetic variation. Monitoring the presence of microorganisms that are dangerous to humans, such as pathogenic and drug-resistant E. coli, and identifying reservoirs of such strains is important for assessing the risk associated with their spread and possible infection.

2. Results

2.1. Identification of E. coli

The identification of all putative E. coli isolates grown on MacConkey medium was confirmed by PCR by using two marker genes, i.e., uidA and uspA. Glucuronidase production was also confirmed in all isolates by a phenotypic test using TBX agar. The presence of E. coli was recorded in 32 of 67 faecal samples (47.8%). A total of 32 strains of E. coli were isolated, each from a different stool sample. The incidence of E. coli was highest for snake samples (51.6%, 16 isolates/31 samples) and slightly lower for turtles (44.4%, 8/18) and lizards (44.4%, 8/18) (Table 1); however, these differences were not statistically significant (P = 0.881, chi2 = 0.253, contingency coefficients = 0.06). There were also no significant differences between the frequency of E. coli in carnivorous (48.5%, 16 isolates/33 samples), herbivorous (45.4%, 10/22) and omnivorous (54.5%, 6/11) reptiles (P = 0.886, chi2 = 0.243, contingency coefficients = 0.06).

2.2. Detection of Virulence-Associated Genes

Genes encoding virulence factors were detected in 50% (n = 16) E. coli strains. The most commonly found were traT (found in 37.5% of isolates, n = 12), encoding complement resistant protein, fyuA (21.87%, n = 7), coding for the ferric yersiniabactin uptake receptor, and irp-2 (15.62%, n = 5), responsible for biosynthesis of the siderophore yersiniabactin. Other virulence genes were detected less frequently, i.e., vat (vacuolating autotransporter toxin, 9.37%, n = 3), pic (serine protease pic autotransporter, which shows proteolytic activity on complement system proteins, 6.25%, n = 2), iucD (aerobactin siderophore synthesis, 3%, n = 1) and astA (enteroaggregative E. coli heat-stable toxin EAST1, 3.12%, n = 1) (Table 2).
In seven isolates, the coexistence of between two and four virulence genes was recorded and, interestingly, as many as six of these strains were obtained from snakes (Table 2). Moreover, 1 strain (16a, from a corn snake) with a pic-astA profile can be classified as IPEC, 1 strain (41, from milk snake) with a pic-irp-2-traT-fyuA profile is a hybrid strain (the pic gene is characteristic of IPEC, and irp-2, traT and fyuA for ExPEC), while the remaining strains contain virulence genes characteristic of ExPEC. No E. coli strain contained any of the remaining virulence genes characteristic of either IPEC (stx1, stx2, hlyA, eaeA, saa, escV, ent, bfpB, elt, estIa, estIb, invE, ipaH, and aggR) or ExPEC (pap-C, ompT, cva/cvi, iss, iutA, and kpsII).

2.3. Antibiotic Susceptibility Testing

Most of the E. coli isolates (71.87%, 23/32) were found to be susceptible to all of the tested antimicrobial agents. Among the remaining isolates, the phenotype of resistance to streptomycin (21.87%, 7/32) was the most frequent, while resistance to other substances, i.e., gentamicin (6.25%, 2/32), amikacin (6.25%, 2/32), tetracycline (6.25%, 2/32), ampicillin (3.12% 1/32), amoxicillin/clavulanic acid (3.12%, 1/32), and trimethoprim (3.12%. 1/32), was observed much less frequently. A total of 3 strains (9.37%) showed resistance to more than 1 drug, but only 1 strain (3.125%), E. coli 37a from corn snake, was classified as multidrug-resistant (MDR, defined as resistance to at least 3 or more antibiotics belonging to different antimicrobial categories). It is also interesting that intermediate streptomycin susceptibility was recorded for the majority of the strains tested (59.37%, 19/32). The 2 strains that showed resistance to AMC or AMP and 4 strains with intermediate susceptibility to AMP were susceptible to cephalosporins (2G, 3G and 4G) and carbapenems (Table 2).

2.4. Detection of Resistance Genes

The occurrence of the resistance genes was recorded for only 4 (12.5%) E. coli strains. Moreover, 1 strain (3.12%) was detected with the aadA gene encoding streptomycin/spectinomycin adenylyltransferase, 2 strains (6.25%) were confirmed with the tetA, tetB and tetM genes conferring resistance to tetracyclines, and 1 isolate (3.12%) carried the TEM-type beta-lactamase (blaTEM). For each of these strains, a correlation was found between the phenotype and the presence of resistance genes (Table 2).

2.5. Determination of E. coli Phylogenetic Groups

Phylogenetic groups of E. coli were determined based on the electrophoretic profiles of multiplex PCR amplicons (yja, TspE4.C2, chuA, svg and uidA) (Figure 1). Over 65% of the isolates (65.6%, n = 21) belonged to group B1, 18.7% (n = 6) were assigned to group A, 9.4% (n = 3) to B2, and 6.2% (n = 2) to group D. None of the isolates belonged to group B21 (no isolate contained the svg gene) (Figure 1). It is worth noting that all the strains representing group B2 were collected from snakes (Table 2).

2.6. Rep-PCR Fingerprinting

DNA amplification with the (GTG)5 primer revealed significant genotypic diversity among the E. coli isolates. The electrophoretic profiles were reproducible in the 2 separate PCR reactions, and the fingerprinting generated distinct amplification bands ranging in size from 350 bp to 2700 bp. The profiles obtained contained between 2 and 12 PCR products and allowed the 32 strains to be grouped into 27 rep-types (Figure 2). This indicates considerable genetic diversity of the E. coli isolates, with a maximum percentage of dissimilarity of 41%. There was no correlation between the electrophoretic profile and the phylogenetic group or the host species, although several strains representing the same phylogenetic group had identical fingerprints (Figure 3).

3. Discussion

The scientific literature abounds with information on the drug susceptibility and virulence of E. coli isolated from humans, mammals, and birds, but reports on the occurrence and characteristics of E. coli in reptiles are scarce. The prevalence of the E. coli strains reported in these studies (47.8%) is in line with that reported by Gopee et al. [11] (52%) for reptiles from the Emperor Valley Zoo, Trinidad, Spain. The authors recorded the highest incidence of E. coli in Crocodylidae (83.3%), lower incidences in turtles (68.08%) and snakes (37.5–51.1%, depending on the family), and the lowest for lizards (33.3%). In contrast with our observations, a higher frequency of E. coli was recorded in herbivorous reptiles (66.7%) than in carnivorous reptiles (46.4%).
A significantly higher frequency of E. coli in captive and free-living reptiles was recorded by Ramos et al. [3] in Brazil (68%) and, as in our study, they reported a higher incidence of E. coli in snakes (84.4%) than in lizards (46.6%) and turtles (40–50%). Similar results were obtained by Sylvester et al. [12] and Bautista-Trujillo et al. [10], who showed the presence of E. coli in approximately 40% of green iguanas. Several other authors have also described the presence of E. coli in reptiles; however, they did not take into account the frequency of this bacterium in individual groups of reptiles. Książczyk et al. [4] isolated a total of 35 E. coli strains from 103 stool samples from reptiles at the zoo in Wrocław, Poland. These data indicate 34% prevalence, assuming each strain was isolated from a different sample. Much higher prevalence of E. coli in reptiles is suggested by the findings of Unger et al. [13], who isolated a total of 142 E. coli isolates from 150 faecal samples derived from >60 exotic reptile species crossing the veterinary border at Frankfurt airport.
Warm-blooded animals (mammals and birds) are already known to be reservoirs of both IPEC and ExPEC E. coli strains [14,15,16], but knowledge of the occurrence of pathogenic E. coli in cold-blooded animals is scant. In terms of the presence of virulence genes, our research is largely in line with the findings of Ramos et al. [3], who detected only 2 of the many virulence genes characteristic for ETEC, EHEC and NTEC, i.e., the cnf1 gene encoding cytotoxic necrotizing factor 1 (found in 9.2% of RepEC isolates) and the astA gene encoding EAST1 toxin (2.6%). The frequency described in this study (3.12%) of the astA gene, coding for heat-stable enterotoxin EAST1 characteristic of EAEC, is also consistent with results reported by Książczyk et al. [4]. In the case of virulence genes characteristic for IPEC, it should be emphasized that our research is the first report on the presence of the pic gene in RepEC, characteristic for the EHEC pathotype. Completely different results were presented by Bautista-Trujillo et al. [10], who classified 62 of 100 E. coli isolates from captive green iguanas in Mexico as diarrhoeagenic E. coli (DEC) strains. Among them, STEC strains (40.3%) carrying the stx1 (38.7%) or stx2 (1.6%) gene were the most prevalent pathotype, followed by EAEC (27.4%, aap-positive strains), ETEC (27.4% each, lt- or st-positive) and EPEC (4.9%, bfpA- or eaeA-positive). The frequency of tratT (37.5%, coding for complement resistance protein) and fyuA (21.87%, coding for the ferric yersiniabactin uptake receptor) genes reported in these studies was about twice as high as in the case of RepEC strains isolated in Poland (traT-16.7%, fyuA-8.3%) by another research team [4]. The fyuA gene is common in both UPEC and APEC strains, while the traT gene is characteristic of the UPEC pathotype [4]. The occasional presence of the irp-2 gene in RepEC (8.3%) was also demonstrated by Książczyk et al. [4]. However, in contrast to our results, they did not detect the iucD or vat gene in any strain, and in several strains, they confirmed the presence of other virulence genes characteristic of ExPEC, i.e., papC, iss and tsh.
The results of this study on drug resistance in E. coli most closely match the findings of Sylvester et al. [12], who showed that among E. coli strains from wild and pet green iguanas (from Grenada), resistance to streptomycin (12%) and amoxicillin-clavulanic acid (12%) was the most common, and only 7% of strains were multi-drug resistant. A much higher prevalence of resistant E. coli strains was recorded in reptiles housed at zoo in Trinidad [11], and resistance to ampicillin (66.7%), tetracycline (57.6%) and gentamicin (12.1%) was the most frequent. Interestingly, the prevalence of streptomycin resistance (36.4%) demonstrated by these studies was somewhat similar to our data (21.9%). An agreement was also noted in the lack of resistance to fluoroquinolones. A high percentage of resistant E. coli strains was also demonstrated by Bautista-Trujillo et al. [10], who classified as many as 82.3% of DEC isolates (from captive green iguanas in Mexico) as MDR, and most frequently noted resistance to amikacin (~40%) and ampicillin (~30%).
The higher incidence of drug-resistant strains in captive reptiles from Trinidad and Mexico may be due to different veterinary practices and the different prevalence of drug-resistant E. coli in the reptile diet and environment. Details on the type and frequency of antibiotic use in reptiles in these countries are not known, but Mexico was among the top ten countries in the world with the highest use of antibiotics in veterinary medicine in 2017 [16].
The higher frequency of resistance to streptomycin (~22%) compared to resistance to other antimicrobial substances (0–6.25%) reported in these studies may be due to the widespread use of this antibiotic in veterinary medicine as well as in horticulture as a plant protection product. The resulting streptomycin-resistant strains may spread in the environment and eventually be acquired by the reptiles via their feed (plants, rodents, meat). Moreover, the results obtained in this study and some literature data suggest that the reptilian Enterobacteriaceae may have a reduced susceptibility to streptomycin. Streptomycin resistance was the most frequently reported antibiotic resistance phenotype in reptile-derived Salmonella strains in Poland (25%) [17] and Indonesia (75%) [18]. Similarly, Bertolini et al. [19] showed a high percentage (79%) of Salmonella strains intermediately susceptible to streptomycin in reptiles.
According to our best knowledge, this work is the first full report of the occurrence of resistance genes in E. coli from reptiles. The aadA (aminoglycoside (3″) (9) adenylyltransferase), tetA (tetracycline efflux protein TetA), tetB (tetracycline efflux protein TetB) and blaTEM genes detected in several RepEC strains are commonly found in drug-resistant Enterobacteriaceae isolated from humans and farm animals [20,21]. The tetM gene coding for tetracycline ribosomal protection protein has thus far been found sporadically in E. coli, mainly in pig and chicken strains [21]. Its presence confirmed in this work in one RepEC isolate may be the result of genetic transfer from Enterococcus or Lactobacillaceae, common carriers of tetM [22,23]. The TEM family of β-lactamases covers a large group of enzymes and determining which TEM lactamase has been detected in these studies requires more detailed sequencing-based analyzes. In E. coli strains, the most common is the prototype narrow-spectrum blaTEM-1 lactamase, from which the broad-spectrum lactamases (ESBL) have evolved by mutation [24,25]. The blaTEM-1 gene was also found in two strains of E. coli from reptiles transported across the veterinary border in Frankfurt, where it coexisted with many other resistance genes [13].
Phylogenetic group B1, which dominated (65.6%) among the RepEC isolates, is characteristic of non-pathogenic E. coli strains, while groups A and B2, recorded in this study for a total of 9 isolates, is characteristic of ExPEC, i.e., UPEC and APEC [4,26]. It is therefore worth noting that 3 of 4 RepEC strains, containing 4 virulence genes simultaneously, belonged to the B2 phylogenetic group. The results presented in this study are consistent with the reports of Gordon and Cowling [27], who assigned nearly 70% of E. coli isolates from snakes and lizards to phylogenetic group B1, while strains of groups A, B2 and D accounted for 15.1%, 6.1% and 9.1%, respectively. A higher percentage (25%) of strains representing the B2 phylogenetic group was recorded in turtles [28]. A higher frequency of strains of the B1 group in RepEC was reported by Ramos et al. [3] (88.4%) and Książczyk et al. [4] (95.8%).

4. Materials and Methods

4.1. Collection of Faecal Samples

The research material consisted of faecal samples collected between 2017 and 2020 from 67 reptiles, i.e., snakes (n = 31), lizards (n = 18) and turtles (n = 18) living in captivity or as pets. The animals did not show any symptoms of disease. They were obtained from private owners (n = 53) and from pet shops (n = 14) in the Lubelskie Province, Poland.

4.2. E. coli Isolation

Stool specimens collected with a swab were suspended in peptone water and incubated for approximately 18 hours, inoculated onto MacConkey agar (Oxoid Ltd., Altrincham, UK), and incubated at 37 °C for 24 h under aerobic conditions. Single pink colonies were inoculated on TSB (trypticase soy broth) (Oxoid Ltd., Altrincham, UK), and pure cultures supplemented with 20% glycerol were stored at −80 °C for further analysis. To determine the ability of the strains to produce glucuronidase, they were additionally plated on TXB agar and incubated ~24 h at 44 °C.

4.3. Identification of E. coli

Presumptive E. coli isolates were identified based on detection of the uidA glucuronidase gene and the E. coli-specific flanking region of the uspA gene (coding for universal stress protein). PCR was performed using DreamTaq polymerase (Thermo Fisher Scientific, Vilnius, Lithuania) and the following two pairs of primers: uidAF-TATGGAATTTCGCCGATTTT and uidAR-TGTTTGCCTCCCTGCTGCGG (uidA amplicon size 166 bp) and uspAF-CCGATACGCTGCCAATCAGT and uspAR-ACGCAGACCGTAGGCCAGAT (uspA amlicon 884 bp) [25]. The PCR reaction were performed using Dream Taq polymerase and the following thermal-cycling program: initial denaturation at 94 °C for 5 min; 30 cycles of 94 °C for 45 s, 57 °C for 45 s and 72 °C for 1 min; final extension step at 72 °C for 8 min. PCR products (8 µL, ~600 ng) were separated by electrophoresis (100 V, 1 h) on 1.8% agarose gels and visualized by SimplySafe (Eurx, Gdańsk, PL) staining. PCR product sizes were determined by comparison with a Nova 100 bp DNA ladder (Novazym, Poznań, Poland) using Image Lab 6.1. Software (Bio-Rad, Hercules, CA, USA).

4.4. DNA Extraction

Whole-genome DNA was extracted from all of the tested E. coli isolates (n = 32) using the Gene MATRIX Bacterial & Yeast Genomic DNA Purification Kit (Eurx, Gdańsk, Popland) following the manufacturer’s instructions and stored at −20 °C. DNA concentration was determined by measuring the absorbance of 2 µL of the sample at 260/280  nm using the NanoDrop Lite spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA), and the quality of DNA was analyzed by agarose (1.5% w/v) gel electrophoresis. No signs of DNA degradation (smearing) were observed in any of the samples, and its concentration was ~20 ng/µL.

4.5. Detection of Virulence Genes

Uniplex or multiplex PCR (Table 3), using gene-specific primers (Table S1), was used to detect the presence of 29 genes associated with virulence in both intestinal and extraintestinal pathogenic E. coli strains. Two E. coli strains from wild mammals previously confirmed to have some virulence genes [16], i.e., astA-stx1-stx2-hlyA (E. coli 22a) and astA-escV-eaeA (E. coli 19), were used as positive controls. All PCR reactions were performed in an Eppendorf Mastercycler using Dream Taq polymerase (Thermo Scientific). PCR products (8 µL, ~600 ng) were separated by electrophoresis (100 V, 1 h) on 1.8% agarose gels and visualized by SimplySafe (Eurx, Gdańsk, Poland) staining. PCR product sizes were determined by comparison with a Nova 100 bp DNA ladder (Novazym, Poznań, Poland) using Image Lab 6.1. Software (Bio-Rad, Herkules, CA, USA).

4.6. Antimicrobial Susceptibility Testing

Antibiotic susceptibility testing was performed by the agar disk diffusion method according to Clinical and Laboratory Standards Institute (CLSI) recommendations [31]. Isolates were revitalized on tryptone soy agar (TSA) plates (Oxoid, Altrincham, UK). Bacterial suspensions with a turbidity equivalent to McFarland Standard 0.5 were swabbed on Müeller Hinton agar plates (Oxoid, Altrincham, UK) with a sterile cotton swab. Antibiotic disks (Oxoid, Altrincham, UK) containing ampicillin (AMP, 10 µg), amoxicillin/clavulanic acid (AMC, 20/10 µg), cefotaxime (CTX, 30 µg), chloramphenicol (C, 30 µg), tetracycline (TE, 30 µg), trimethoprim (W, 5 µg), ciprofloxacin (CIP, 5 µg), streptomycin (S, 10 µg), gentamicin (CN, 10 µg), amikacin (AK, 30 µg) and nitrofurantoin (F, 100 µg) were placed on the plates. Strains that showed resistance or intermediate susceptibility to AMP or AMC were additionally tested against cefoxitin (FOX, 30 µg), cefepime (FEP 30 µg), ceftazidime (CAZ 30 µg), ceftazidime/clavulanic acid (CZC, 30/10 µg), imipenem (IMP, 10 µg) and meropenem (MEM, 10 µg). Inhibition zone diameters, including the diameter of the 6-mm disks, were measured after incubation at 35 °C for 20 h. Isolates were classified as resistant I, intermediate (I), and susceptible (S) according to breakpoints provided by CLSI [31]; only in the case of nitrofurantoin was the interpretation of the results based on EUCAST guidelines [32]. E. coli ATCC 25922 reference strain was used as a quality control.

4.7. Detection of Resistance Genes

All of hte E. coli isolates that showed phenotypic resistance or intermediate susceptibility to specific antimicrobial agents were tested for the presence of relevant resistance genes using a uniplex or multiplex PCR assay. Specifically, genes conferring resistance to beta-lactams (blaTEM, blaOXY, blaCTX and blaSHV), tetracyclines (tetA, tetB, tetC, tetK, tetL, tetM and tetO), aminoglycosides (aadA, strA/strB, aac(3)-II, aac(3)-IV, aphA1 and aphA2), trimethoprim and sulfonamides (sul1, sul2, sul3, dfrA1, dfrA5, and drfA7-A17) were detected using the primers and annealing temperatures shown in Table S2. Analysis of the PCR products was performed as above.

4.8. Determination of E. coli Phylogenetic Groups

To determine the phylogenetic groups of the E. coli isolates, five sets of primers for the five sets of primers for the genes yja, TspE4.C2, chuA, svg and uidA (Table S3) were used in a multiplex PCR, as previously described [33]. PCR products were separated by electrophoresis in 2.5% (wt/vol) high resolution agarose (Blirt, Gdańsk, Poland). The amplicons sizes were determined by comparison with a Nova 100 bp DNA ladder (Novazym, Poznań, Poland) using Image Lab 6.1. Software (Bio-Rad, Herkules, CA, USA). The phylogenetic groups were determined based on the PCR gel pattern [33].

4.9. Rep-PCR Fingerprinting

The genetic diversity of E. coli isolates was assessed by repetitive extragenic palindromic (REP)-PCR using the (GTG)5 primer -5′-GTGGTGGTGGTGGTG-3′ [34]. The PCR reactions were carried out in 18 µL of a reaction mixture containing 7.5 µL of 2x PCR Mix Plus (A&A Biotechnology, Gdynia, Poland), 0.75 µL of (GTG)5 primer, 0.75 µL (~20 ng/µL) of DNA template, and 9 µL of H2O. Amplification was performed in the following sequence: initial denaturation 5 min, 95 °C; 40 cycles at 95 °C for 45 s, 42 °C for 35 s and 72 °C for 2 min; and final extension at 72 °C for 8 min. PCR products were separated by electrophoresis in 1.5% agarose. The gels were run at 80 V in 1X TBE buffer, stained with SimplySafe (Eurx, Gdańsk, Poland), and visualized under an UV source using the GelDoc Go imaging system (BioRad, Herkules, CA, USA). Profiles were analysed with Image Lab Software (BioRad, Herkules, CA, USA) by comparison with the Nova 100 bp DNA ladder (Novazym, Poznań, Poland).
The presence of a given band was coded as 1 and the absence of a given band was coded as 0 in a data matrix (Excel, Microsoft Office), and the unweighted pair group method with arithmetic averages (UPGMA) was used to generate a dendrogram of dissimilarity in Statistica software (Ver. 13.1, Tulsa, OK, USA).

4.10. Statistical Analysis

Pearson’s chi-squared test was used to determine the relationship between diet (carnivores, omnivores, herbivores) and the frequency of E. coli, as well as between the type of reptile (snakes, lizards, turtles) and the frequency of E. coli isolation. The level of significance was set as p < 0.05. The statistical analysis was performed using Microsoft Excel 2019.

5. Conclusions

The results presented in this study contribute to knowledge of the occurrence and phenotypic and genotypic features of RepEC strains. Although the incidence of drug resistance in E. coli strains from reptiles in Poland is low, the possibility of transmission of resistance genes to other members of Enterobacteriaceae, including Salmonella, which commonly inhabits reptile intestines, should be taken into account. In addition, RepEC strains contain virulence genes characteristic of ExPEC, and less often of IPEC. IPEC strains are diarrhoeagenic pathogens, while ExPEC strains may exist as commensals, but under favourable conditions can cause infections. Due to the pathogenic potential of RepEC strains, they may pose a health risk to humans, and reptile keepers should use good hygiene practices when handling these animals.

Supplementary Materials

The following are available online at https://www.mdpi.com/article/10.3390/pathogens11020127/s1, Table S1: Sequences of primers used to detect virulence genes, amplicon sizes and references [14,29,30]; Table S2: Sequences of primers used to detect resistance genes [14,23,35,36,37]; Table S3: Primer sequences used to determine phylogenetic groups in E. coli strains [35].

Author Contributions

Conceptualization, M.D.; methodology, M.D. and B.T.; validation, D.S.-P.; formal analysis, M.D.; investigation, M.D., D.S.-P.; resources, M.D and K.S.; data curation, M.D.; writing—original draft preparation, M.D.; writing—review and editing, R.U.-C. and B.T.; visualization, M.D.; funding acquisition, R.U.-C. and K.S. All authors have read and agreed to the published version of the manuscript.

Funding

This research received no external funding.

Institutional Review Board Statement

The research was carried out in accordance with the principles of bioethics in force in Poland (Act of 15 January 2015 on the protection of animals used for scientific or educational purposes, Journal of Laws 2015 item 266) as well as in the EU (Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes), according to which collecting stool samples for scientific research is not a procedure on animals and does not require the approval of the Local Ethical Committee. Pet and pet store owners have agreed to collect reptile feces when cleaning terrariums without interacting with animal welfare.

Informed Consent Statement

Not applicable.

Data Availability Statement

All the data presented in the study are included in the article. Further enquires can be directed to the corresponding author.

Acknowledgments

Not applicable.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. FEDIAF (The European Pet Food Industry). FEDIAF Annual Report 2021. p. 44, 50. Available online: https://www.fediaf.org/annual-report.html (accessed on 20 January 2022).
  2. Valdez, J.W. Using Google Trends to Determine Current, Past, and Future Trends in the Reptile Pet Trade. Animals 2021, 11, 676. [Google Scholar] [CrossRef]
  3. Ramos, C.P.; Santana, J.A.; Morcatti Coura, F.; Xavier, R.G.C.; Leal, C.A.G.; Oliveira Junior, C.A.; Heinemann, M.B.; Lage, A.P.; Lobato, F.C.F.; Silva, R.O.S. Identification and Characterization of Escherichia coli, Salmonella Spp., Clostridium perfringens, and C. difficile Isolates from Reptiles in Brazil. Biomed. Res. Int. 2019, 2019, 9530732. [Google Scholar] [CrossRef] [Green Version]
  4. Książczyk, M.; Dudek, B.; Kuczkowski, M.; O’Hara, R.; Korzekwa, K.; Wzorek, A.; Korzeniowska-Kowal, A.; Upton, M.; Junka, A.; Wieliczko, A.; et al. The Phylogenetic Structure of Reptile, Avian and Uropathogenic Escherichia coli with Particular Reference to Extraintestinal Pathotypes. Int. J. Mol. Sci. 2021, 22, 1192. [Google Scholar] [CrossRef]
  5. Lindstedt, B.A.; Finton, M.D.; Porcellato, D.; Brandal, L.T. High frequency of hybrid Escherichia coli strains with combined Intestinal Pathogenic Escherichia coli (IPEC) and Extraintestinal Pathogenic Escherichia coli (ExPEC) virulence factors isolated from human faecal samples. BMC Infect. Dis. 2018, 18, 544. [Google Scholar] [CrossRef]
  6. Wasiński, B. Extra-intestinal pathogenic Escherichia coli—Threat connected with food-borne infections. Ann. Agric. Environ. Med. 2019, 26, 532–537. [Google Scholar] [CrossRef]
  7. García, A.; Fox, J.G. A One Health Perspective for Defining and Deciphering Escherichia coli Pathogenic Potential in Multiple Hosts. Comp. Med. 2021, 71, 3–45. [Google Scholar] [CrossRef]
  8. Rodriguez-Siek, K.E.; Giddings, C.W.; Doetkott, C.; Johnson, T.J.; Fakhr, M.K.; Nolan, L.K. Comparison of Escherichia coli isolates implicated in human urinary tract infection and avian colibacillosis. Microbiology 2005, 151, 2097–2110. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  9. Gomes, T.A.; Elias, W.P.; Scaletsky, I.C.; Guth, B.E.; Rodrigues, J.F.; Piazza, R.M.; Ferreira, L.C.; Martinez, M.B. Diarrheagenic Escherichia coli. Braz. J. Microbiol. 2016, 47, 3–30. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  10. Bautista-Trujillo, G.U.; Gutiérrez-Miceli, F.A.; Mandujano-García, L.; Oliva-Llaven, M.A.; Ibarra-Martínez, C.; Mendoza-Nazar, P.; Ruiz-Sesma, B.; Tejeda-Cruz, C.; Pérez-Vázquez, L.C.; Pérez-Batrez, J.E.; et al. Captive Green Iguana Carries Diarrheagenic Escherichia coli Pathotypes. Front. Vet. Sci. 2020, 7, 99. [Google Scholar] [CrossRef]
  11. Gopee, N.V.; Adesiyun, A.A.; Caesar, K. A longitudinal study of Escherichia coli strains isolated from captive mammals, birds, and reptiles in Trinidad. J. Zoo Wildl. Med. Off. Publ. Am. Assoc. Zoo Vet. 2000, 31, 353–360. [Google Scholar] [CrossRef]
  12. Sylvester, W.R.B.; Amadi, V.; Hegamin-Younger, C.; Pinckey, R.; Macpherson, C.N.L.; Mckibben, J.S.; Bruhl-Day, R.; John-Sylvester, K.D.; Hariharan, H. Occurrence of Antibiotic Resistant Escherichia coli in Green Iguanas (Iguana Iguana) in Grenada, West Indies. Int. J. Vet. Med. Res. Rep. 2014, 2014, 260412. [Google Scholar] [CrossRef]
  13. Unger, F.; Eisenberg, T.; Prenger-Berninghoff, E.; Leidner, U.; Ludwig, M.L.; Rothe, M.; Semmler, T.; Ewers, C. Imported reptiles as a risk factor for the global distribution of Escherichia coli harbouring the colistin resistance gene mcr-1. Int. J. Antimicrob. Agents 2017, 49, 122–123. [Google Scholar] [CrossRef] [PubMed]
  14. Nowaczek, A.; Dec, M.; Stępień-Pyśniak, D.; Urban-Chmiel, R.; Marek, A.; Różański, P. Antibiotic Resistance and Virulence Profiles of Escherichia coli Strains Isolated from Wild Birds in Poland. Pathogens 2021, 10, 1059. [Google Scholar] [CrossRef] [PubMed]
  15. Turchi, B.; Dec, M.; Bertelloni, F.; Winiarczyk, S.; Gnat, S.; Bresciani, F.; Viviani, F.; Cerri, D.; Fratini, F. Antibiotic Susceptibility and Virulence Factors in Escherichia coli from Sympatric Wildlife of the Apuan Alps Regional Park (Tuscany, Italy). Microb. Drug Resist. 2019, 25, 772–780. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  16. Tiseo, K.; Huber, L.; Gilbert, M.; Robinson, T.P.; Van Boeckel, T.P. Global Trends in Antimicrobial Use in Food Animals from 2017 to 2030. Antibiotics 2020, 9, 918. [Google Scholar] [CrossRef]
  17. Zając, M.; Skarżyńska, M.; Lalak, A.; Kwit, R.; Śmiałowska-Węglińska, A.; Pasim, P.; Szulowski, K.; Wasyl, D. Salmonella in Captive Reptiles and Their Environment-Can We Tame the Dragon? Microorganisms 2021, 9, 1012. [Google Scholar] [CrossRef]
  18. Arnafia, W.; Ningrum, S.G.; Adji, R.S.; Lukman, D.W.; Pasaribu, F.H.; Wibawan, I.W.T. Isolation of Salmonella from reptiles in pet shop and its susceptibility to antibiotics in Indonesia. Hum. Vet. Med. 2016, 8, 177–181. [Google Scholar]
  19. Bertelloni, F.; Chemaly, M.; Cerri, D.; Gall, F.L.; Ebani, V.V. Salmonella infection in healthy pet reptiles: Bacteriological isolation and study of some pathogenic characters. Acta Microbiol. Immunol. Hung. 2016, 63, 203–216. [Google Scholar] [CrossRef] [Green Version]
  20. Mazurek, J.; Pusz, P.; Bok, E.; Stosik, M.; Baldy-Chudzik, K. The phenotypic and genotypic characteristics of antibiotic resistance in Escherichia coli populations isolated from farm animals with different exposure to antimicrobial agents. Pol. J. Microbiol. 2013, 62, 173–179. [Google Scholar] [CrossRef]
  21. Jurado-Rabadán, S.; de la Fuente, R.; Ruiz-Santa-Quiteria, J.A.; Orden, J.A.; de Vries, L.E.; Agersø, Y. Detection and linkage to mobile genetic elements of tetracycline resistance gene tet(M) in Escherichia coli isolates from pigs. BMC Vet. Res. 2014, 10, 155. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  22. Dec, M.; Stępień-Pyśniak, D.; Gnat, S.; Fratini, F.; Urban-Chmiel, R.; Cerri, D.; Winiarczyk, S.; Turchi, B. Antibiotic Susceptibility and Virulence Genes in Enterococcus Isolates from Wild Mammals Living in Tuscany, Italy. Microb. Drug Resist. 2020, 26, 505–519. [Google Scholar] [CrossRef]
  23. Dec, M.; Nowaczek, A.; Stępień-Pyśniak, D.; Wawrzykowski, J.; Urban-Chmiel, R. Identification and antibiotic susceptibility of lactobacilli isolated from turkeys. BMC Microbiol. 2018, 18, 168. [Google Scholar] [CrossRef] [PubMed]
  24. Ur Rahman, S.; Ali, T.; Ali, I.; Khan, N.A.; Han, B.; Gao, J. The Growing Genetic and Functional Diversity of Extended Spectrum Beta-Lactamases. Biomed. Res. Int. 2018, 2018, 9519718. [Google Scholar] [CrossRef] [PubMed]
  25. Paterson, D.L.; Bonomo, R.A. Extended-spectrum beta-lactamases: A clinical update. Clin. Microbiol. Rev. 2005, 18, 657–686. [Google Scholar] [CrossRef] [Green Version]
  26. Xu, X.; Sun, Q.; Zhao, L. Virulence Factors and Antibiotic Resistance of Avian Pathogenic Escherichia Coli in Eastern China. J. Vet. Res. 2019, 63, 317–320. [Google Scholar] [CrossRef] [Green Version]
  27. Gordon, D.M.; Cowling, A. The distribution and genetic structure of Escherichia coli in Australian vertebrates: Host and geographic effects. Microbiology 2003, 149, 3575–3586. [Google Scholar] [CrossRef] [Green Version]
  28. Godambe, L.P.; Bandekar, J.; Shashidhar, R. Species specific PCR based detection of Escherichia coli from Indian foods. 3 Biotech 2017, 7, 130. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  29. Ewers, C.; Janssen, T.; Kiessling, S.; Philipp, H.C.; Wieler, L.H. Rapid detection of virulence-associated genes in avian pathogenic Escherichia coli by multiplex polymerase chain reaction. Avian Dis. 2005, 49, 269–273. [Google Scholar] [CrossRef]
  30. Tóth, I.; Hérault, F.; Beutin, L.; Oswald, E. Production of cytolethal distending toxins by pathogenic Escherichia coli strains isolated from human and animal sources: Establishment of the existence of a new cdt variant (Type IV). J. Clin. Microbiol. 2003, 41, 4285–4291. [Google Scholar] [CrossRef] [Green Version]
  31. CLSI. Performance Standards for Antimicrobial Susceptibility Testing, 28th ed.; CLSI Supplement M100; Clinical and Laboratory Standards Institute: Wayne, PA, USA, 2018. [Google Scholar]
  32. EUCAST. The European Committee on Antimicrobial Susceptibility Testing. Breakpoint Tables for Interpretation of MICs and Zone Diameters. Version 11.0. 2021. Available online: http://www.eucast.org (accessed on 10 December 2021).
  33. Feng, Y.; Mannion, A.; Madden, C.M.; Swennes, A.G.; Townes, C.; Byrd, C.; Marini, R.P.; Fox, J.G. Cytotoxic Escherichia coli strains encoding colibactin and cytotoxic necrotizing factor (CNF) colonize laboratory macaques. Gut Pathog. 2017, 9, 71. [Google Scholar] [CrossRef]
  34. Cruz-Soto, A.S.; Toro-Castillo, V.; Munguía-Magdaleno, C.O.; Torres-Flores, J.E.; Flores-Pantoja, L.E.; Loeza-Lara, P.D.; Jiménez-Mejía, R. Genetic relationships, biofilm formation, motility and virulence of Escherichia coli isolated from bovine mastitis. Rev. Mex. Cienc. Pecu. 2020, 11, 167–182. [Google Scholar] [CrossRef]
  35. Fang, H.; Ataker, F.; Hedin, G.; Dornbusch, K. Molecular epidemiology of extended-spectrum beta-lactamases among Escherichia coli isolates collected in a Swedish hospital and its associated health care facilities from 2001 to 2006. J. Clin. Microbiol. 2008, 46, 707–712. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  36. Kozak, G.K.; Boerlin, P.; Janecko, N.; Reid-Smith, R.J.; Jardine, C. Antimicrobial resistance in Escherichia coli isolates from swine and wild small mammals in the proximity of swine farms and in natural environments in Ontario, Canada. Appl. Environ. Microbiol. 2009, 75, 559–566. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  37. Grape, M.; Motakefi, A.; Pavuluri, S.; Kahlmeter, G. Standard and real-time multiplex PCR methods for detection of trimethoprim resistance dfr genes in large collections of bacteria. Clin. Microbiol. Infect. 2007, 13, 1112–1118. [Google Scholar] [CrossRef] [Green Version]
Pathogens 11 00127 g001 550
Figure 1. Multiplex PCR patterns for E. coli phylogenetic groups.
Figure 1. Multiplex PCR patterns for E. coli phylogenetic groups.
Pathogens 11 00127 g001
Pathogens 11 00127 g002 550
Figure 2. Agarose gel patterns of products amplified by (GTG)5-PCR.
Figure 2. Agarose gel patterns of products amplified by (GTG)5-PCR.
Pathogens 11 00127 g002
Pathogens 11 00127 g003 550
Figure 3. Dendrogram generated by UPGMA clustering from (GTG)5-PCR fingerprinting of E. coli strains.
Figure 3. Dendrogram generated by UPGMA clustering from (GTG)5-PCR fingerprinting of E. coli strains.
Pathogens 11 00127 g003
Table
Table 1. Frequency of isolation of Escherichia coli from captive reptiles in Poland.
Table 1. Frequency of isolation of Escherichia coli from captive reptiles in Poland.
Group of Reptiles Number of SamplesSpecies (Number of Samples) Diet GroupNumber of E. coli Isolates (%)
Snakes31Pantheropsis guttatus (14)
Python regius (3)
Boa constrictor (4)
Lampropeltis triangulum (4)
Morelia pilota (1)
Orthrophis teaniurus (5)		Carnivore
Carnivore
Carnivore
Carnivore
Carnivore
Carnivore		7 (50)
3 (100)
3 (75)
2 (50)
1 (100)
0
Lizards18Pogona vitticeps (6)
Iguana iguana (4)
Eublepharis macularius (3)
Furcifer pardali (5)		Omnivore
Herbivore
Carnivore
Omnivore		5 (83)
2 (50)
0
1 (20)
Turtles 18Testudo horsfieldii (8)
Testudo hermanni (9)
Chelonoidis carbonaria (1)		Herbivore
Herbivore
Herbivore 		5 (62.5)
2 (22)
1 (100)
Total:67 32 (47.8)
Table
Table 2. Phenotypic and genotypic drug resistance profiles, virulence profiles and phylogenetic groups of E. coli strains isolated from reptiles.
Table 2. Phenotypic and genotypic drug resistance profiles, virulence profiles and phylogenetic groups of E. coli strains isolated from reptiles.
IsolateSpeciesPhylogenetic GroupAntibiotic Phenotype Pattern (Including Resistant and Intermediate Strains) aResistance GenesVirulence Genes
6Corn snake (Pantheropsis guttatus)B1AMP-AK-S-CIP-traT
13aBall python (Python regius)B1AMP-irp-2, iucD, traT, fyuA
15aBall python (Python regius)A---
16aCorn snake (Pantheropsis guttatus)AAMP-AMC-AK-S-pic, astA
26Boa constrictor snake (Boa constrictor)B1S--
30aCorn snake (Pantheropsis guttatus)B2S-irp-2, vat, traT, fyuA
31aCorn snake (Pantheropsis guttatus)B1SaadAtraT
32aMilk snake (Lampropeltis triangulum)B2S-irp-2, vat, traT, fyuA
34Ball python (Python regius)B1S-traT
35aBoa constrictor snake (Boa constrictor)DS-vat
36aBoa constrictor snake (Boa constrictor)A--traT
37aCorn snake (Pantheropsis guttatus)B1CN-AK-S-T-Wb--
39Corn snake (Pantheropsis guttatus)B1 --
41Milk snake (Lampropeltis triangulum)B2S-pic, irp-2, traT, fyuA
47Corn snake (Pantheropsis guttatus)B1S--
48Carpet python (Morelia spilota)B1S-traT, fyuA
54aPanther chameleon (Furcifer pardalis)B1S--
46Central bearded dragon (Pogona vitticeps)AS-TtetA-
55Central bearded dragon (Pogona vitticeps)DCN-AK-S-T-CIPtetB, tetMirp-2, fyuA
61Central bearded dragon (Pogona vitticeps)B1AMP-SblaTEMtraT
62Central bearded dragon (Pogona vitticeps)B1S--
63Central bearded dragon (Pogona vitticeps)B1S--
44Green iguana (Iguana iguana)B1S-traT
65Green iguana (Iguana iguana)B1AMP-S--
17aSteppe tortoise (Testudo horsfieldii)B1--traT
18aSteppe tortoise (Testudo horsfieldii)AS-fyuA
19aSteppe tortoise (Testudo horsfieldii)A---
25aRed-footed tortoise (Chelonoidis carbonaria)B1AK-S--
67Greek tortoise (Testudo hermanni)B1S--
69Steppe tortoise (Testudo horsfieldii)B1S--
70Steppe tortoise (Testudo horsfieldii)B1S--
71Greek tortoise (Testudo hermanni)B1S--
a bold symbols indicate resistance, non-bold symbols indicate intermediate susceptibility; AMP—ampicillin, AMC—amoxicillin/clavulanic acid, AK—amikacin, CIP—ciprofloxacin, CN—gentamicin, S—streptomycin, T—tetracycline, W—trimethoprim; b MDR strain.
Table
Table 3. PCR schemes used to detect virulence genes in E. coli.
Table 3. PCR schemes used to detect virulence genes in E. coli.
Detected GenesAnnealing Temperature (°C)Reference
Multiplex Istx1, stx2, hylA, eaeA, saa65 (10 cycles) then
62 (20 cycles)		[14]
Multiplex IIecsV, ent, bfpB, invE, astA, aggR, pic, ipaH, elt, estIa, estIb62[14]
Multiplex IIIompT, iutA63[14]
Multiplex IVtsh, pap-C, iss, irp-257[29]
Multiplex ViucD, vat54
Uniplex Icva/cvi58
Uniplex IIcnf55[30]
Uniplex IIIkpsII56[4]
Uniplex IVtraT59
Uniplex VfyuA59
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Dec, M.; Stepien-Pysniak, D.; Szczepaniak, K.; Turchi, B.; Urban-Chmiel, R. Virulence Profiles and Antibiotic Susceptibility of Escherichia coli Strains from Pet Reptiles. Pathogens 2022, 11, 127. https://doi.org/10.3390/pathogens11020127

AMA Style

Dec M, Stepien-Pysniak D, Szczepaniak K, Turchi B, Urban-Chmiel R. Virulence Profiles and Antibiotic Susceptibility of Escherichia coli Strains from Pet Reptiles. Pathogens. 2022; 11(2):127. https://doi.org/10.3390/pathogens11020127

Chicago/Turabian Style

Dec, Marta, Dagmara Stepien-Pysniak, Klaudiusz Szczepaniak, Barbara Turchi, and Renata Urban-Chmiel. 2022. "Virulence Profiles and Antibiotic Susceptibility of Escherichia coli Strains from Pet Reptiles" Pathogens 11, no. 2: 127. https://doi.org/10.3390/pathogens11020127

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop