Next Article in Journal
Targeting Host Tyrosine Kinase Receptor EPHA2 Signaling Affects Uropathogen Infection in Human Bladder Epithelial Cells
Next Article in Special Issue
The Troublesome Ticks Research Protocol: Developing a Comprehensive, Multidiscipline Research Plan for Investigating Human Tick-Associated Disease in Australia
Previous Article in Journal
Consumption of Galactose by Trypanosoma cruzi Epimastigotes Generates Resistance against Oxidative Stress
Previous Article in Special Issue
Investigation of Rickettsia conorii in Patients Suspected of Having Crimean-Congo Hemorrhagic Fever
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Pathogens
Volume 11
Issue 10
10.3390/pathogens11101175
pathogens-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Genetic Diversity of Coxiella burnetii in Iran by Multi-Spacer Sequence Typing

by
Ashraf Mohabati Mobarez
1,
Neda Baseri
2,3,
Mohammad Khalili
4,
Ehsan Mostafavi
2,3,
John Stenos
5 and
Saber Esmaeili
2,3,*
1
Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran 1411713116, Iran
2
Department of Epidemiology and Biostatics, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran 1316943551, Iran
3
National Reference Laboratory for Plague, Tularemia and Q Fever, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Akanlu, KabudarAhang, Hamadan 6556153145, Iran
4
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman 7616913439, Iran
5
Australian Rickettsial Reference Laboratory, University Hospital Geelong, Geelong, VIC 3220, Australia
*
Author to whom correspondence should be addressed.
Pathogens 2022, 11(10), 1175; https://doi.org/10.3390/pathogens11101175
Submission received: 29 August 2022 / Revised: 2 October 2022 / Accepted: 8 October 2022 / Published: 11 October 2022
(This article belongs to the Collection Updates on Rickettsia and Coxiella)
Browse Figures
Versions Notes

Abstract

Coxiella burnetii, the zoonotic agent of Q fever, has a worldwide distribution including Iran. However, no information regarding the circulating genotype of this infection has been reported in Iran. This study aimed to evaluate the genetic diversity of C. burnetii in Iran using the multi-spacer sequence typing (MST) method. First, 14 positive C. burnetii samples (collected from four sheep, three goats, and seven cattle) were confirmed using quantitative polymerase chain reaction (qPCR) targeting the IS1111 gene. Then, ten spacers (Cox 2, 5, 18, 20, 22, 37, 51, 56, 57, and 61) were amplified using PCR for future MST analysis. The in-silico MST genotyping analysis of domestic ruminant samples revealed two new alleles (Cox5.11 and Cox56.15) in Cox5 and Cox56 loci that led to the emergence of four novel MST genotypes (MST62, 63, 64, and 65) and one MST genotype that has been previously described (MST61). This study showed the circulation of five MST C. burnetii genotypes among Iranian domestic ruminants. Understanding the C. burnetii genotypic profiles is critical in determining and preventing Q fever outbreaks.
Keywords: Coxiella burnetii; Q fever; Genotyping; MST; Iran Coxiella burnetii; Q fever; Genotyping; MST; Iran

Share and Cite

MDPI and ACS Style

Mohabati Mobarez, A.; Baseri, N.; Khalili, M.; Mostafavi, E.; Stenos, J.; Esmaeili, S. Genetic Diversity of Coxiella burnetii in Iran by Multi-Spacer Sequence Typing. Pathogens 2022, 11, 1175. https://doi.org/10.3390/pathogens11101175

AMA Style

Mohabati Mobarez A, Baseri N, Khalili M, Mostafavi E, Stenos J, Esmaeili S. Genetic Diversity of Coxiella burnetii in Iran by Multi-Spacer Sequence Typing. Pathogens. 2022; 11(10):1175. https://doi.org/10.3390/pathogens11101175

Chicago/Turabian Style

Mohabati Mobarez, Ashraf, Neda Baseri, Mohammad Khalili, Ehsan Mostafavi, John Stenos, and Saber Esmaeili. 2022. "Genetic Diversity of Coxiella burnetii in Iran by Multi-Spacer Sequence Typing" Pathogens 11, no. 10: 1175. https://doi.org/10.3390/pathogens11101175

APA Style

Mohabati Mobarez, A., Baseri, N., Khalili, M., Mostafavi, E., Stenos, J., & Esmaeili, S. (2022). Genetic Diversity of Coxiella burnetii in Iran by Multi-Spacer Sequence Typing. Pathogens, 11(10), 1175. https://doi.org/10.3390/pathogens11101175

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop