Quantitative Trait Locus Mapping for Fusarium Wilt Race 4 Resistance in a Recombinant Inbred Line Population of Pima Cotton (Gossypium Barbadense)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Development of the RIL Population
2.2. Experimental Designs, Inoculation Methods, and Assessment of FOV 4 Resistance
2.3. Analysis of Variance
2.4. Linkage Mapping and QTL Analysis
3. Results and Discussion
3.1. Analysis of Variance of FOV4 Resistance Based on DSR
3.2. Performance of the RILs for FOV4 Resistance
3.3. QTL Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Sources of Variation | df | Mean Squares |
---|---|---|
Genotype | 162 | 0.85 ** |
Test | 1 | 0.21 ** |
Genotype × Test | 162 | 0.52 ** |
Error | 324 | 0.38 |
CV (%) | 31.2 | |
h2 | 0.65 |
Test | Mean DSR | Mean DI (%) | Mean Mortality (%) |
---|---|---|---|
1 | 1.65 a | 82.18 a | 0.20 a |
2 | 4.67 b | 93.81 a | 83.65 b |
Line | Test 1 | Test 2 | Line | Test 1 | Test 2 |
---|---|---|---|---|---|
10 most susceptible lines | 10 most susceptible lines | ||||
NMPRIL21 | 0.25 | 1.58 | NMPRIL6 | 2.63 | 3.95 |
NMPRIL20 | 0.38 | 2.06 | NMRIL15 | 2.62 | 5.00 |
NMPRIL24 | 0.44 | 2.22 | NMRIL140 | 2.50 | 4.67 |
NMPRIL135 | 0.46 | 2.34 | NMRIL72 | 2.46 | 5.00 |
NMPRIL12 | 0.48 | 2.41 | NMRIL67 | 2.41 | 5.00 |
NMPRIL7 | 0.50 | 2.05 | NMRIL91 | 2.33 | 5.00 |
NMPRIL55 | 0.60 | 2.04 | NMRIL82 | 2.29 | 4.86 |
NMPRIL65 | 0.67 | 2.01 | NMRIL75 | 2.27 | 5.00 |
NMPRIL71 | 0.72 | 1.98 | NMRIL101 | 2.25 | 4.83 |
NMPRIL100 | 0.78 | 2.01 | NMRIL110 | 2.25 | 4.35 |
LSD (0.05) | 1.12 | 1.18 |
QTL Name | Chr. | Left Marker | Right Marker | Physical Location (Mb) | LOD | PVE (%) | Direction |
---|---|---|---|---|---|---|---|
qFOV.c14-1 | c14 | BNL3932-300 | BNL1897B-300 | 6.25–69.61 | 22.54 | 16.54 | Pima S-6 |
qFOV.c17-1 | c17 | BNL3408a-200 | CIR347-280 | 48.63–48.81 | 21.54 | 29.01 | Pima S-6 |
qFOV.c17-2 | c17 | BNL3408a-200 | CIR347-280 | 48.63–48.81 | 21.54 | 29.01 | Pima S-6 |
qFOV.c19-1 | c19 | BNL1611-430 | DPL0071 | 10.56–21.19 | 11.58 | 18.57 | Pima S-6 |
qFOV.c21-1 | c21 | BNL1053a-230 | BNL3997-430 | 17.52–26.24 | 9.98 | 15.00 | Pima S-6 |
qFOV.c24-1 | c24 | MUSS260a-300 | BNL2961b-400 | 56.77–56.97 | 10.25 | 19.87 | Pima S-6 |
qFOV.c24-2 | c24 | BNL2961b-400 | BNL1521-400 | 56.77–56.97 | 11.89 | 19.87 | Pima S-6 |
qFOV.c25-1 | c25 | MUSS122 | JESPR056 | 52.85 | 13.54 | 21.84 | Pima S-6 |
qFOV.c25-2 | c25 | MUSS122 | JESPR056 | 52.85 | 13.54 | 21.84 | Pima S-6 |
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Abdelraheem, A.; Zhu, Y.; Zhang, J. Quantitative Trait Locus Mapping for Fusarium Wilt Race 4 Resistance in a Recombinant Inbred Line Population of Pima Cotton (Gossypium Barbadense). Pathogens 2022, 11, 1143. https://doi.org/10.3390/pathogens11101143
Abdelraheem A, Zhu Y, Zhang J. Quantitative Trait Locus Mapping for Fusarium Wilt Race 4 Resistance in a Recombinant Inbred Line Population of Pima Cotton (Gossypium Barbadense). Pathogens. 2022; 11(10):1143. https://doi.org/10.3390/pathogens11101143
Chicago/Turabian StyleAbdelraheem, Abdelraheem, Yi Zhu, and Jinfa Zhang. 2022. "Quantitative Trait Locus Mapping for Fusarium Wilt Race 4 Resistance in a Recombinant Inbred Line Population of Pima Cotton (Gossypium Barbadense)" Pathogens 11, no. 10: 1143. https://doi.org/10.3390/pathogens11101143
APA StyleAbdelraheem, A., Zhu, Y., & Zhang, J. (2022). Quantitative Trait Locus Mapping for Fusarium Wilt Race 4 Resistance in a Recombinant Inbred Line Population of Pima Cotton (Gossypium Barbadense). Pathogens, 11(10), 1143. https://doi.org/10.3390/pathogens11101143