Detection and Characterization of Clinical Bordetella trematum Isolates from Chronic Wounds
Abstract
:1. Introduction
2. Results
2.1. Origin of the Isolates
2.2. Identification
2.3. Susceptibility Testing
2.4. Genome Analysis
3. Discussion
4. Materials and Methods
4.1. Identification
4.2. Susceptibility Testing
4.3. Sequencing and Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Isolate 1 | Isolate 2 | Isolate 3 | Isolate 1 | Isolate 2 | Isolate 3 | |
---|---|---|---|---|---|---|
Ampicillin | <2 | >16 | <2 | 2 | >256 | 3 |
Ampicillin/Sulbactam | <2/4 | >16/4 | <2/4 | 1.5 | 6 | 1.5 |
Amikacin | <4 * | <4 * | <4 * | |||
Cefepime | 2 | 8 | 4 | |||
Ceftriaxone | 3 | 4 | 4 | |||
Cefotaxime | >2 | >2 | >2 | |||
Ceftazidime | 4 | 4 | 4 | 4 | 6 | 4 |
Ceftazidime/Avibactam | 4/4 | 4/4 | 4/4 | |||
Ceftolozane/Tazobactam | >8/4 | >8/4 | >8/4 | |||
Chloramphenicol | 16 ** | 16 ** | 16 ** | |||
Ciprofloxacin | 2 | 2 | 2 | 1.5 | 1.5 | 1 |
Colistin | <1 ** | <1 ** | <1 ** | |||
Fosfomycin | >64 ** | >64 ** | >64 ** | |||
Gentamicin | <1 | 2 | 2 | |||
Imipenem | <1 | <1 | <1 | |||
Levofloxacin | 1 | 1 | 1 | |||
Meropenem | <0.125 | <0.125 | <0.125 | 0.047 | 0.047 | 0.047 |
Piperacillin | <8 * | <8 * | <8 * | |||
Piperacillin/ Tazobactam | <2/4 | <2/4 | <2/4 | 0.75 | 1.5 | 0.75 |
Temocillin | 128 ** | 128 ** | 128 ** | |||
Tigecycline | <0.25 | <0.25 | <0.25 | |||
Tobramycin | <1 * | <1 * | 2 | |||
Trimethoprim/Sulfamethoxazole | <1/19 ** | >4/76 ** | <1/19 ** | 0.094 ** | >32 ** | 0.125 ** |
Subsystem Feature Counts | Isolate | B1 | B2 | B3 |
---|---|---|---|---|
Antibiotic/ Toxin Res.: | Copper homeostasis | 8 | 7 | 7 |
Bile hydrolysis | 2 | 2 | 2 | |
Cobalt-zinc- cadmium res. | 5 | 6 | 5 | |
Aminoglycoside adenylyltr. | 1 | 1 | 1 | |
Copper tolreance | 3 | 3 | 3 | |
Fosfomycin resistance | 1 | 1 | 1 | |
Fluoroquinolone resistance | 2 | 2 | 2 | |
Beta-lactamase | 0 | 1 | 0 | |
Chromioum compound res. | 1 | 2 | 1 | |
Invasion/ Intracellular Res.: | SSU ribosomal proteins | 4 | 4 | 4 |
Mycob. Virulence operon inv. in DNA transcr. | 2 | 2 | 2 | |
LSU ribosomal proteins | 3 | 3 | 3 |
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Buechler, C.; Neidhöfer, C.; Hornung, T.; Neuenhoff, M.; Parčina, M. Detection and Characterization of Clinical Bordetella trematum Isolates from Chronic Wounds. Pathogens 2021, 10, 966. https://doi.org/10.3390/pathogens10080966
Buechler C, Neidhöfer C, Hornung T, Neuenhoff M, Parčina M. Detection and Characterization of Clinical Bordetella trematum Isolates from Chronic Wounds. Pathogens. 2021; 10(8):966. https://doi.org/10.3390/pathogens10080966
Chicago/Turabian StyleBuechler, Christian, Claudio Neidhöfer, Thorsten Hornung, Marcel Neuenhoff, and Marijo Parčina. 2021. "Detection and Characterization of Clinical Bordetella trematum Isolates from Chronic Wounds" Pathogens 10, no. 8: 966. https://doi.org/10.3390/pathogens10080966