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Volume 10
Issue 4
10.3390/pathogens10040396
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Article

Nanopore-Sequencing Characterization of the Gut Microbiota of Melolontha melolontha Larvae: Contribution to Protection against Entomopathogenic Nematodes?

by
Ewa Sajnaga
1,*,
Marcin Skowronek
1,
Agnieszka Kalwasińska
2,
Waldemar Kazimierczak
1,
Karolina Ferenc
3,
Magdalena Lis
1 and
Adrian Wiater
4
1
Centre for Interdisciplinary Research, Laboratory of Biocontrol, Application and Production of EPN, John Paul II Catholic University of Lublin, Konstantynów 1J, 20-708 Lublin, Poland
2
Department of Environmental Microbiology and Biotechnology, Nicolaus Copernicus University in Toruń, Lwowska 1, 87-100 Toruń, Poland
3
Centre for Biomedicine Research, Warsaw University of Life Sciences, Nowoursynowska 100, 02-797 Warsaw, Poland
4
Department of Industrial and Environmental Microbiology, Institute of Biological Sciences, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland
*
Author to whom correspondence should be addressed.
Pathogens 2021, 10(4), 396; https://doi.org/10.3390/pathogens10040396
Submission received: 2 March 2021 / Revised: 23 March 2021 / Accepted: 24 March 2021 / Published: 25 March 2021
(This article belongs to the Special Issue Harnessing Host-Pathogen-Microbiota Interactions for Sustainable Disease Management)
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Abstract

This study focused on the potential relationships between midgut microbiota of the common cockchafer Melolontha melolontha larvae and their resistance to entomopathogenic nematodes (EPN) infection. We investigated the bacterial community associated with control and unsusceptible EPN-exposed insects through nanopore sequencing of the 16S rRNA gene. Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetes were the most abundant bacterial phyla within the complex and variable midgut microbiota of the wild M. melolontha larvae. The core microbiota was found to include 82 genera, which accounted for 3.4% of the total number of identified genera. The EPN-resistant larvae differed significantly from the control ones in the abundance of many genera belonging to the Actinomycetales, Rhizobiales, and Clostridiales orders. Additionally, the analysis of the microbiome networks revealed different sets of keystone midgut bacterial genera between these two groups of insects, indicating differences in the mutual interactions between bacteria. Finally, we detected Xenorhabdus and Photorhabdus as gut residents and various bacterial species exhibiting antagonistic activity against these entomopathogens. This study paves the way to further research aimed at unravelling the role of the host gut microbiota on the output of EPN infection, which may contribute to enhancement of the efficiency of nematodes used in eco-friendly pest management.
Keywords: Melolontha melolontha; entomopathogenic nematodes; gut microbiota; host protection; metataxonomics; Xenorhabdus; Photorhabdus; pest biocontrol Melolontha melolontha; entomopathogenic nematodes; gut microbiota; host protection; metataxonomics; Xenorhabdus; Photorhabdus; pest biocontrol
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MDPI and ACS Style

Sajnaga, E.; Skowronek, M.; Kalwasińska, A.; Kazimierczak, W.; Ferenc, K.; Lis, M.; Wiater, A. Nanopore-Sequencing Characterization of the Gut Microbiota of Melolontha melolontha Larvae: Contribution to Protection against Entomopathogenic Nematodes? Pathogens 2021, 10, 396. https://doi.org/10.3390/pathogens10040396

AMA Style

Sajnaga E, Skowronek M, Kalwasińska A, Kazimierczak W, Ferenc K, Lis M, Wiater A. Nanopore-Sequencing Characterization of the Gut Microbiota of Melolontha melolontha Larvae: Contribution to Protection against Entomopathogenic Nematodes? Pathogens. 2021; 10(4):396. https://doi.org/10.3390/pathogens10040396

Chicago/Turabian Style

Sajnaga, Ewa, Marcin Skowronek, Agnieszka Kalwasińska, Waldemar Kazimierczak, Karolina Ferenc, Magdalena Lis, and Adrian Wiater. 2021. "Nanopore-Sequencing Characterization of the Gut Microbiota of Melolontha melolontha Larvae: Contribution to Protection against Entomopathogenic Nematodes?" Pathogens 10, no. 4: 396. https://doi.org/10.3390/pathogens10040396

APA Style

Sajnaga, E., Skowronek, M., Kalwasińska, A., Kazimierczak, W., Ferenc, K., Lis, M., & Wiater, A. (2021). Nanopore-Sequencing Characterization of the Gut Microbiota of Melolontha melolontha Larvae: Contribution to Protection against Entomopathogenic Nematodes? Pathogens, 10(4), 396. https://doi.org/10.3390/pathogens10040396

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