Search Results (6)

(1) Background: Beetles in the superfamily Scarabaeoidea are one of the most important groups of Coleoptera. They are found in various ecosystems all over the world and belong to coprophagous, necrophagous, saproxylophagous, phyllophagous and rhizophagous types. The aim of the study is to describe the fauna and distribution of Scarabaeoidea in the Republic of Mordovia (central part of European Russia); (2) Methods: The study was conducted from 2003 to 2023. Collection material (specimens from 1972 and 1986) was also used. Specimens were collected using traditional Coleoptera collecting methods (manual collecting, light trap, collection of rotten remains and pitfall traps). For each observation, the coordinates, number of specimens and dates were recorded; (3) Results: The dataset contains 3198 occurrences. We examined 11,011 specimens of Scarabaeoidea. The dataset contains information on 88 species of Scarabaeoidea. Of these, five species (Aphodius pedellus, Nobius serotinus, Phaeaphodius rectus, Planolinus fasciatus and Onthophagus medius) are listed for the region for the first time. Another seven species are additionally known from others taken from the literature (but were not found during the present field survey); (4) Conclusions: Species diversity of Scarabaeoidea of Mordovia accounts for 95 species from 4 families (Geotrupidae, Trogidae, Lucanidae and Scarabaeidae). Ten species (Protaetia marmorata, Anoplotrupes stercorosus, Cetonia aurata, Protaetia cuprea volhyniensis, Oxythyrea funesta, Platycerus caraboides, Serica brunnea, Melolontha hippocastani, Trichius fasciatus and Protaetia fieberi) constitute the main population of Scarabaeoidea fauna given in the dataset. The species diversity of Scarabaeoidea of the Republic of Mordovia is roughly similar in number of species to that of neighboring or more northern regions. Full article

This study focused on the potential relationships between midgut microbiota of the common cockchafer Melolontha melolontha larvae and their resistance to entomopathogenic nematodes (EPN) infection. We investigated the bacterial community associated with control and unsusceptible EPN-exposed insects through nanopore sequencing of the 16S rRNA gene. Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetes were the most abundant bacterial phyla within the complex and variable midgut microbiota of the wild M. melolontha larvae. The core microbiota was found to include 82 genera, which accounted for 3.4% of the total number of identified genera. The EPN-resistant larvae differed significantly from the control ones in the abundance of many genera belonging to the Actinomycetales, Rhizobiales, and Clostridiales orders. Additionally, the analysis of the microbiome networks revealed different sets of keystone midgut bacterial genera between these two groups of insects, indicating differences in the mutual interactions between bacteria. Finally, we detected Xenorhabdus and Photorhabdus as gut residents and various bacterial species exhibiting antagonistic activity against these entomopathogens. This study paves the way to further research aimed at unravelling the role of the host gut microbiota on the output of EPN infection, which may contribute to enhancement of the efficiency of nematodes used in eco-friendly pest management. Full article

The efficacy of two strains of two Beauveria species (B. bassiana and B. brongniartii), individually or as co-inoculants, to control Melolontha sp. grubs was assessed in two organic strawberry plantations in relation to the environmental conditions, their abundance after soil inoculation, and their in vitro chitinolytic activity, thereby also verifying their impact on soil microbial communities. A reduction of the grubs’ damage to strawberry plants was observed when compared to the untreated control in one plantation, irrespective of the strain used and whether they were applied as single or as co-inoculum. The metabolic pattern expressed by the two fungi in vitro was different: B. bassiana showed a higher metabolic versatility in the use of different carbon sources than B. brongniartii, whose profile was partly overlapped in the co-inoculum. Similar differences in the chitinolytic activity of each of the fungi and the co-inoculum were also pointed out. A higher abundance of B. bassiana in the soils receiving this species in comparison to those receiving B. brongniartii, together with its in vitro metabolic activity, could account for the observed diverse efficacy of pest damage control of the two species. However, environmental and climatic factors also affected the overall efficacy of the two bioinocula. According to the monitoring of the two species in soil, B. bassiana could be considered as a common native species in the studied locations in contrast to B. brongniartii, which seemed to be a non-endemic species. Nevertheless, the inoculation with both species or the co-inoculum did not consistently affect the soil microbial (fungi and bacteria) biodiversity, as expressed by the operational taxonomic unit (OTU) number and Shannon–Wiener diversity index based on terminal restriction fragment length polymorphism (TRFLP) data. A small transient increase of the share of the inoculated species to the total fungal community was noted by the analysis of genes copy numbers only for B. brongniartii at the end of the third growing season. Full article

The effect of fungal entomopathogen M. anisopliae strain NCAIM 362 against M. melolontha larvae in sweet potato was tested under open field conditions when crop management included compost supply and soil cover (agro-foil or agro-textile). Additionally, the effect of M. anisopliae same strain against M. melolontha was compared with the effect of α-cypermethrin under greenhouse conditions. Soil microbial community using Illumina sequencing and soil biological activity were tested as possible parameter influencing M. anisopliae effect. According to the results, compost supply and textile cover may enhance the effectiveness of M. anisopliae under open field conditions, while no effect of fungal treatment was detected under greenhouse conditions. Even if soil parameters (chemical composition, bacterial, and biological activity) were identical, the effect of α-cypermethrin against M. melolontha larvae was significant: lower ratio of larval survival and less damaged tubers were detected after the chemical treatment. Our results suggest that M. anisopliae strain NCAIM 362 is not effective to control M. melolontha larvae, further pieces of research are needed to test other species of the Metarhizium genus to find an effective agent for sustainable pest control in sweet potato. Full article

The mechanisms of action of the complex including entomopathogenic nematodes of the genera Steinernema and Heterorhabditis and their mutualistic partners, i.e., bacteria Xenorhabdus and Photorhabdus, have been well explained, and the nematodes have been commercialized as biological control agents against many soil insect pests. However, little is known regarding the nature of the relationships between these bacteria and the gut microbiota of infected insects. In the present study, 900 bacterial isolates that were obtained from the midgut samples of Melolontha melolontha larvae were screened for their antagonistic activity against the selected species of the genera Xenorhabdus and Photorhabdus. Twelve strains exhibited significant antibacterial activity in the applied tests. They were identified based on 16S rRNA and rpoB, rpoD, or recA gene sequences as Pseudomonas chlororaphis, Citrobacter murliniae, Acinetobacter calcoaceticus, Chryseobacterium lathyri, Chryseobacterium sp., Serratia liquefaciens, and Serratia sp. The culture filtrate of the isolate P. chlororaphis MMC3 L3 04 exerted the strongest inhibitory effect on the tested bacteria. The results of the preliminary study that are presented here, which focused on interactions between the insect gut microbiota and mutualistic bacteria of entomopathogenic nematodes, show that bacteria inhabiting the gut of insects might play a key role in insect resistance to entomopathogenic nematode pressure. Full article

Root-feeding Melolonthinae larvae are a forest pest species in Europe that can exert serious damage. In Poland, they are classified as the most dangerous pest on land dedicated to afforestation and the most serious threat to natural regeneration in the stands. This study was performed in three forest districts in east Poland (Lubartów, Marcule, and Wyszków forest districts) in mixed conifer forests, where the presence of Melolontha spp. grubs was evaluated in autumn and spring of 2012 to 2017, respectively. In spring 2012, 2014, and 2016, consecutively ‘small sawdust pits’ in rows between seedlings were prepared, and in adjacent inter-rows similar control pits without sawdust were marked. In spring and autumn of the following year, sawdust and soil from both types of pits were sieved and Melolonthinae larvae were counted and compared. More grubs were found in sawdust pits in spring than in autumn. In Marcule Forest District (FD) (2014), more grubs were found in inter-rows than in rows with seedlings, when compared to grubs detected using the traditional method of “autumn large pits assessment”, recommended by Polish forest rules. The Melolonthinae population size and location of grubs were related to the weather conditions in the evaluated periods, as well. We conclude that to properly assess the cockchafer threat, it is necessary to perform spring assessment and to search in inter-rows. This could be recommended for decisions on control. Full article