Next Article in Journal
Virulence Factors in Coagulase-Negative Staphylococci
Previous Article in Journal
Comparison of Cytokine Expression Profile in Chikungunya and Dengue Co-Infected and Mono-Infected Patients’ Samples
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Pathogens
Volume 10
Issue 2
10.3390/pathogens10020168
pathogens-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Alterations of Fucosyltransferase Genes and Fucosylated Glycans in Gastric Epithelial Cells Infected with Helicobacter pylori

by
Ruyue Fan
,
Xiurui Han
,
Yanan Gong
,
Lihua He
,
Zhijing Xue
,
Yaming Yang
,
Lu Sun
,
Dongjie Fan
,
Yuanhai You
,
Fanliang Meng
,
Xiaomei Yan
,
Maojun Zhang
and
Jianzhong Zhang
*
State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
*
Author to whom correspondence should be addressed.
Pathogens 2021, 10(2), 168; https://doi.org/10.3390/pathogens10020168
Submission received: 11 January 2021 / Revised: 31 January 2021 / Accepted: 1 February 2021 / Published: 4 February 2021
Browse Figures
Versions Notes

Abstract

Helicobacter pylori (H. pylori) adhesion to human gastric epithelial cells is closely linked with fucosylated glycans. Therefore, investigation of fucosylation in the interaction of gastric epithelial cells with H. pylori is critical. In this study we used lectin microarrays to detect the expression of fucosylated glycans in gastric epithelial cells (GES-1) infected with H. pylori strains isolated from patients with different diseases including chronic gastritis, duodenal ulcers, and gastric cancer (each containing two strains) at 4 h. In addition, we investigated the time-course expression of fucosyltransferase (FUT) 1–6 genes in GES-1 cells stimulated with H. pylori strains at 0.5–8 h. At 4 h post-infection, Lotus, AAA, BC2LCN, PA-IIL, CNL and ACG lectins had increased signals in H. pylori-infected GES-1 cells compared to uninfected cells. Higher expression of FUT1 and FUT2 was detected in all H. pylori-infected GES-1 cells within 2 h, regardless of the H. pylori strain. In particular, the expression of FUT2 was higher in H. pylori-infected GES-1 cells with a higher fold change in levels of BC2LCN lectin specific to α1-2 linked fucose (Fuc) at 4 h. The results suggest that the high levels of α1, 2-linked Fuc synthesized by FUT1/2, might play a role in the preliminary stage of H. pylori infection. This provides us with pivotal information to understand the adhesion of H. pylori to human gastric epithelial cells.
Keywords: H. pylori; fucosyltransferase expression; fucosylation; gastric epithelial cells H. pylori; fucosyltransferase expression; fucosylation; gastric epithelial cells

Share and Cite

MDPI and ACS Style

Fan, R.; Han, X.; Gong, Y.; He, L.; Xue, Z.; Yang, Y.; Sun, L.; Fan, D.; You, Y.; Meng, F.; et al. Alterations of Fucosyltransferase Genes and Fucosylated Glycans in Gastric Epithelial Cells Infected with Helicobacter pylori. Pathogens 2021, 10, 168. https://doi.org/10.3390/pathogens10020168

AMA Style

Fan R, Han X, Gong Y, He L, Xue Z, Yang Y, Sun L, Fan D, You Y, Meng F, et al. Alterations of Fucosyltransferase Genes and Fucosylated Glycans in Gastric Epithelial Cells Infected with Helicobacter pylori. Pathogens. 2021; 10(2):168. https://doi.org/10.3390/pathogens10020168

Chicago/Turabian Style

Fan, Ruyue, Xiurui Han, Yanan Gong, Lihua He, Zhijing Xue, Yaming Yang, Lu Sun, Dongjie Fan, Yuanhai You, Fanliang Meng, and et al. 2021. "Alterations of Fucosyltransferase Genes and Fucosylated Glycans in Gastric Epithelial Cells Infected with Helicobacter pylori" Pathogens 10, no. 2: 168. https://doi.org/10.3390/pathogens10020168

APA Style

Fan, R., Han, X., Gong, Y., He, L., Xue, Z., Yang, Y., Sun, L., Fan, D., You, Y., Meng, F., Yan, X., Zhang, M., & Zhang, J. (2021). Alterations of Fucosyltransferase Genes and Fucosylated Glycans in Gastric Epithelial Cells Infected with Helicobacter pylori. Pathogens, 10(2), 168. https://doi.org/10.3390/pathogens10020168

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop