Next Article in Journal
Transcriptome-Based Identification of a Functional Fasciola hepatica Carboxylesterase B
Next Article in Special Issue
Challenges and Tools for In Vitro Leishmania Exploratory Screening in the Drug Development Process: An Updated Review
Previous Article in Journal
In Silico Survey and Characterization of Babesia microti Functional and Non-Functional Proteases
Previous Article in Special Issue
Assessment of the Genetic Diversity of Echinococcus multilocularis from Copro-Isolated Eggs
Article

Evaluation of the Chagas Western Blot IgG Assay for the Diagnosis of Chagas Disease

1
Service de Parasitologie-Mycologie, APHP Sorbonne Université Hôpital Pitié-Salpêtrière, 75013 Paris, France
2
Instituto Multidisciplinario de Investigación en Patologías Pediátricas (IMIPP), CONICET-GCBA, Buenos Aires C1425EFD, Argentina
3
Servicio de Parasitología y Enfermedad de Chagas, Hospital de Niños “Dr. Ricardo Gutiérrez”, Instituto Multidisciplinario de Investigación en Patologías Pediátricas (IMIPP), CONICET-GCBA, Buenos Aires C1425EFD, Argentina
4
UMRS 1135, Centre d’Immunologie et des Maladies Infectieuses, Sorbonne Université, 75013 Paris, France
5
Service des Maladies Infectieuses et Tropicales, APHP, Sorbonne Université Hôpital Pitié-Salpêtrière, 75013 Paris, France
6
Service des Maladies Infectieuses et Tropicales, APHP, Hôpitaux Universitaires Paris Nord val de Seine, Hôpital Bichat, 75000 Paris, France
*
Author to whom correspondence should be addressed.
Academic Editors: Coralie L’Ollivier and Aurélien Dumetre
Pathogens 2021, 10(11), 1455; https://doi.org/10.3390/pathogens10111455
Received: 15 September 2021 / Revised: 4 November 2021 / Accepted: 5 November 2021 / Published: 10 November 2021
(This article belongs to the Special Issue Epidemiology of Parasitic Zoonoses)
Chagas disease is a debilitating and often fatal pathology resulting from infection by the protozoan parasite Trypanosoma cruzi. In its recommendations, the World Health Organization states that the diagnosis of T. cruzi infection is usually based on the detection of antibodies against T. cruzi antigens and performed with two methodologically different assays. An inconclusive result can be resolved with a third “confirmatory” assay. The objective of this article is to evaluate the effectiveness of the Chagas Western Blot IgG assay (LDBio Diagnostics, Lyon, France) as a confirmatory serologic test. The Chagas Western Blot IgG assay was performed with native antigens derived from a T. cruzi strain of the TcVI genotype. Retrospective sera were provided by two parasitology laboratories (France and Argentina). The sensitivity, specificity, positive predictive value and negative predictive value of the Chagas blot were all 100% in our sera collection. The Chagas blot is an easy and qualitative method for the diagnosis of Chagas disease, with results in less than 2 h. This immunoblot has potential as a supplemental test for the confirmation of the presence of antibodies against T. cruzi in serum specimens. Nonetheless, the very good initial results presented here will need to be confirmed in larger studies. View Full-Text
Keywords: chagas disease; diagnostic; Trypanosoma cruzi chagas disease; diagnostic; Trypanosoma cruzi
Show Figures

Figure 1

MDPI and ACS Style

Brossas, J.-Y.; Griselda, B.; Bisio, M.; Guihenneuc, J.; Gulin, J.E.N.; Jauréguiberry, S.; Lescure, F.-X.; Fekkar, A.; Mazier, D.; Altcheh, J.; Paris, L. Evaluation of the Chagas Western Blot IgG Assay for the Diagnosis of Chagas Disease. Pathogens 2021, 10, 1455. https://doi.org/10.3390/pathogens10111455

AMA Style

Brossas J-Y, Griselda B, Bisio M, Guihenneuc J, Gulin JEN, Jauréguiberry S, Lescure F-X, Fekkar A, Mazier D, Altcheh J, Paris L. Evaluation of the Chagas Western Blot IgG Assay for the Diagnosis of Chagas Disease. Pathogens. 2021; 10(11):1455. https://doi.org/10.3390/pathogens10111455

Chicago/Turabian Style

Brossas, Jean-Yves, Ballering Griselda, Margarita Bisio, Jeremy Guihenneuc, Julián E.N. Gulin, Stéphane Jauréguiberry, François-Xavier Lescure, Arnaud Fekkar, Dominique Mazier, Jaime Altcheh, and Luc Paris. 2021. "Evaluation of the Chagas Western Blot IgG Assay for the Diagnosis of Chagas Disease" Pathogens 10, no. 11: 1455. https://doi.org/10.3390/pathogens10111455

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop