An Update on the Laboratory Diagnosis of Rickettsia spp. Infection
Abstract
:1. Introduction
2. Current Challenges in Diagnosis
3. Serology
Disease | Serological Assay | Sensitivity (%) | Specificity (%) | References |
---|---|---|---|---|
Spotted fever group rickettsioses | WFT | 21–70 | 46–96 | [31,41,42,43,44] |
IFA IgM | 83–85 | 100 | [31,33] | |
IFA IgG | 85–100 | 99–100 | [28,31,32,33] | |
ELISA IgM | 91–100 | 94–100 | [32,42] | |
ELISA IgG | 83–100 | 87–100 | [32,41] | |
Murine typhus | WFT | 56–81 | 96–98 | [45] |
IFA IgM | 53–100 | 91–99 | [28,34] | |
IFA IgG | 83–100 | 91–100 | [28,34] | |
ELISA IgM | 45–98 | 91–98 | [34] | |
ELISA IgG | 100 | 83 | [34] |
4. Direct Detection of Rickettsia Species from Clinical Specimens
Assay Type | Specimen | Species | Sensitivity (%) | Specificity (%) | References |
---|---|---|---|---|---|
Immunohistochemistry | Skin | R. rickettsii, SFG rickettsioses | 70 | 100 | [47,48] |
Immunohistochemistry | Eschar | O. tsutsugamushi | 65 | 100 | [50] |
Immunocytochemical | Blood (circulating endothelial cells) | R. conorii | 50 | 94 | [53] |
PCR | Blood | R. rickettsia | 6–69 | 90–100 | [54,55] |
PCR | Eschar | R. rickettsia, R. parkeri | 70–92 | 95–100 | [50,52] |
Isolation | Plasma, buffy coat, skin | R. conorii, O. tsutsugamushi | 59 | - | [55] |
5. Genomic Approaches in the Diagnosis of Rickettsia Species Infection
6. Barriers and Implementation of Contemporary Testing Strategies
7. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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Stewart, A.G.; Stewart, A.G.A. An Update on the Laboratory Diagnosis of Rickettsia spp. Infection. Pathogens 2021, 10, 1319. https://doi.org/10.3390/pathogens10101319
Stewart AG, Stewart AGA. An Update on the Laboratory Diagnosis of Rickettsia spp. Infection. Pathogens. 2021; 10(10):1319. https://doi.org/10.3390/pathogens10101319
Chicago/Turabian StyleStewart, Adam G., and Alexandra G. A. Stewart. 2021. "An Update on the Laboratory Diagnosis of Rickettsia spp. Infection" Pathogens 10, no. 10: 1319. https://doi.org/10.3390/pathogens10101319