A Broad-Based Mosquito Yeast Interfering RNA Pesticide Targeting Rbfox1 Represses Notch Signaling and Kills Both Larvae and Adult Mosquitoes
Abstract
:1. Introduction
2. Results and Discussion
2.1. Silencing Rbfox1 Results in A. aegypti Larval Mortality
2.2. Sugar Baited Delivery of Rbfox1.457 siRNA
2.3. Development of an RNAi-Based Yeast Pesticide That Can Be Deployed as an ATSB
2.4. Silencing Rbfox1 Represses Notch Signaling
2.5. Rbfox1.457 Yeast Pesticides Function as Broad-Range Mosquito Insecticides but Are Not Found to Be Toxic to Select Non-Target Arthropods
3. Materials and Methods
3.1. Mosquito Rearing
3.2. Identification of siRNA #457
3.3. Adult siRNA-ATSB Trials
3.4. Generation of Yeast Interfering RNA Larvicide Strains and Yeast Culturing
3.5. Larvicide Trials
3.5.1. Laboratory Assays
3.5.2. Semi-Field Trials
3.5.3. Yeast ATSB Simulated Field Trials
3.6. Whole Mount In Situ Hybridization
3.7. Evaluation of Non-target Species
3.7.1. O. fasciatus
3.7.2. H. convergens
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Experiment 1 | % Mortality | p Value Control vs. Treatment | n |
---|---|---|---|
Larval soaking | |||
Control siRNA | 0 ± 0 | 4.4−13 | 40 |
Rbfox1 siRNA | 73 ± 2.5 | ||
Adult microinjection | |||
Control siRNA | 5 | 4.0−3 | 20 |
Rbfox1 siRNA | 45 | ||
ATSB/siRNA feeding | |||
Control siRNA | 8 ± 5.3 | 3.5−10 | 37 |
Rbfox1 siRNA | 77 ± 7.1 | 42 |
Feeding Rate (%) | ||||
---|---|---|---|---|
Experiment 1 | Species | Control | Rbfox1.457 | n |
siRNA ATSB | A. aegypti | 57 ± 7 | 63 ± 8.5 | 65 |
Yeast ATSB | A. aegypti | 100 ± 0 | 100 ± 0 | 150 |
A. gambiae | 100 ± 0 | 100 ± 0 | 75 | |
C. quinquefasciatus | 100 ± 0 | 100 ± 0 | 225 | |
A. albopictus | 89 ± 0.5 | 91 ± 1 | 225 |
Test Organism 1 | Control | Rbfox1.457 | n |
---|---|---|---|
D. magna adults | 100 ± 0 | 98 ± 3.5 | 40 |
D. melanogaster larvae | 100 ± 0 | 100 ± 0 | 60 |
D. melanogaster adults | 100 ± 0 | 99 ± 1 | 60 |
H. convergens adults | 90 ± 0 | 90 ± 7 | 20 |
O. fasciatus adults | 80 ± 7 | 77.5 ± 18 | 40 |
T. castaneum adults | 100 ± 0 | 100 ± 0 | 60 |
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Mysore, K.; Sun, L.; Hapairai, L.K.; Wang, C.-W.; Roethele, J.B.; Igiede, J.; Scheel, M.P.; Scheel, N.D.; Li, P.; Wei, N.; et al. A Broad-Based Mosquito Yeast Interfering RNA Pesticide Targeting Rbfox1 Represses Notch Signaling and Kills Both Larvae and Adult Mosquitoes. Pathogens 2021, 10, 1251. https://doi.org/10.3390/pathogens10101251
Mysore K, Sun L, Hapairai LK, Wang C-W, Roethele JB, Igiede J, Scheel MP, Scheel ND, Li P, Wei N, et al. A Broad-Based Mosquito Yeast Interfering RNA Pesticide Targeting Rbfox1 Represses Notch Signaling and Kills Both Larvae and Adult Mosquitoes. Pathogens. 2021; 10(10):1251. https://doi.org/10.3390/pathogens10101251
Chicago/Turabian StyleMysore, Keshava, Longhua Sun, Limb K. Hapairai, Chien-Wei Wang, Joseph B. Roethele, Jessica Igiede, Max P. Scheel, Nicholas D. Scheel, Ping Li, Na Wei, and et al. 2021. "A Broad-Based Mosquito Yeast Interfering RNA Pesticide Targeting Rbfox1 Represses Notch Signaling and Kills Both Larvae and Adult Mosquitoes" Pathogens 10, no. 10: 1251. https://doi.org/10.3390/pathogens10101251