Improvement in the Biological Properties of Titanium Surfaces with Low-Temperature Plasma
Abstract
:1. Introduction
2. Materials and Methods
2.1. Titanium Disk Preparation
2.2. Low-Temperature Plasma Treatment
2.3. Scanning Electron Microscopic Analysis of Surfaces
2.4. Energy Dispersive Spectrometry
2.5. X-Ray Photoelectron Spectroscopy
2.6. Examination of Surface Wettability
2.7. Examination of Surface Roughness
2.8. Cell Viability
2.9. Porphyromonas gingivalis Culture
2.10. Quantification of Substrate and Products
2.11. Bacteria Counting Analysis
2.12. Statistical Analyses
3. Results
3.1. Surfaces Electron Microscopic Analysis
3.2. Energy Dispersive Spectrometry
3.3. X-ray Photoelectron Spectroscopy
3.4. Examination of Surface Wettability
3.5. Surface Roughness Examination
3.6. Cell Viability
3.7. Porphyromonas Gingivalis Adhesion and Growth
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Treatment | Component | ||
---|---|---|---|
Ti | O | C | |
Crtl-Ti | 93.62 | 4.70 | 2.83 |
LTP-Ti | 92.80 | 5.70 | 1.9 |
p-value | 0.302 | 0.131 | * 0.038 |
Treatment | Culturing Days after P. gingivalis Cultured | ||||
---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | |
Crtl-Ti | 63.10 cfu/cm2 | 1584.90 cfu/cm2 | 1,258,925.41 cfu/cm2 | 15,848,931.92 cfu/cm2 | 25,118,864.32 cfu/cm2 |
LTP-Ti | 19.95 cfu/cm2 | 91.20 cfu/cm2 | 316,227.77 cfu/cm2 | 794,328.23 cfu/cm2 | 1,584,893.19 cfu/cm2 |
p-value | *** p < 0.001 | *** p < 0.001 | * p < 0.05 | *** p < 0.001 | * p < 0.05 |
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Pan, Y.-H.; Yao, W.-L.; Lin, J.C.Y.; Salamanca, E.; Tsai, P.-Y.; Leu, S.-J.; Yang, K.-C.; Huang, H.-M.; Teng, N.C.; Chang, W.-J. Improvement in the Biological Properties of Titanium Surfaces with Low-Temperature Plasma. Metals 2019, 9, 943. https://doi.org/10.3390/met9090943
Pan Y-H, Yao W-L, Lin JCY, Salamanca E, Tsai P-Y, Leu S-J, Yang K-C, Huang H-M, Teng NC, Chang W-J. Improvement in the Biological Properties of Titanium Surfaces with Low-Temperature Plasma. Metals. 2019; 9(9):943. https://doi.org/10.3390/met9090943
Chicago/Turabian StylePan, Yu-Hwa, Wan-Ling Yao, Jerry Chin Yi Lin, Eisner Salamanca, Pei-Yo Tsai, Sy-Jye Leu, Kai-Chiang Yang, Haw-Ming Huang, Nai Chia Teng, and Wei-Jen Chang. 2019. "Improvement in the Biological Properties of Titanium Surfaces with Low-Temperature Plasma" Metals 9, no. 9: 943. https://doi.org/10.3390/met9090943