Next Article in Journal
EHMT1/GLP; Biochemical Function and Association with Brain Disorders
Previous Article in Journal
Mining Novel Candidate Imprinted Genes Using Genome-Wide Methylation Screening and Literature Review
 
Search for Articles:
Title / Keyword
Author / Affiliation
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
Journals
Epigenomes
Volume 1
Issue 3
10.3390/epigenomes1030014
epigenomes-logo
Submit to this Journal Review for this Journal Edit a Special Issue
► Article Menu

Article Menu

share announcement question_answer thumb_up
...
textsms
...
Article

Saliva as a Blood Alternative for Genome-Wide DNA Methylation Profiling by Methylated DNA Immunoprecipitation (MeDIP) Sequencing

by
Nicklas Heine Staunstrup
1,2,4,5,*,
Anna Starnawska
1,4,5,
Mette Nyegaard
1,4,
Anders Lade Nielsen
1,4,
Anders Børglum
1,4,5 and
Ole Mors
2,3,4
1
Department of Biomedicine, Aarhus University, 8000 Aarhus C, Denmark
2
Translational Neuropsychiatric Unit, Aarhus University Hospital, 8240 Risskov, Denmark
3
Psychosis Research Unit, Aarhus University Hospital, 8240 Risskov, Denmark
4
The Lundbeck Foundation Initiative for Integrative Psychiatric Research, iPSYCH, Denmark
5
Center for Integrative Sequencing, iSEQ, Aarhus University, 8000 Aarhus C, Denmark
*
Author to whom correspondence should be addressed.
Academic Editor: Muller Fabbri
Epigenomes 2017, 1(3), 14; https://doi.org/10.3390/epigenomes1030014
Received: 31 July 2017 / Revised: 5 October 2017 / Accepted: 11 October 2017 / Published: 19 October 2017
View Full-Text Download PDF
Browse Figures

Abstract

Abstract: Background: Interrogation of DNA methylation profiles hold promise for improved diagnostics, as well as the delineation of the aetiology for common human diseases. However, as the primary tissue of the disease is often inaccessible without complicated and inconvenient interventions, there is an increasing interest in peripheral surrogate tissues. Whereas most work has been conducted on blood, saliva is now becoming recognized as an interesting alternative due to the simple and non-invasive manner of collection allowing for self-sampling. Results: In this study we have evaluated if saliva samples are suitable for DNA methylation studies using methylated DNA immunoprecipitation coupled to next-generation sequencing (MeDIP-seq). This was done by comparing the DNA methylation profile in saliva against the benchmark profile of peripheral blood from three individuals. We show that the output, quality, and depth of paired-end 50 bp sequencing reads are comparable between saliva and peripheral blood and, moreover, that the distribution of reads along genomic regions are similar and follow canonical methylation patterns. Conclusion: In summary, we show that high-quality MeDIP-seq data can be generated using saliva, thus supporting the future use of saliva in the generation of DNA methylation information at annotated genes, non-RefSeq genes, and repetitive elements relevant to human disease. View Full-Text
Keywords: epigenetics; DNA methylation; MeDIP-seq; genome-wide; saliva; blood epigenetics; DNA methylation; MeDIP-seq; genome-wide; saliva; blood
►▼ Show Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Supplementary Material

SciFeed

Share and Cite

MDPI and ACS Style

Staunstrup, N.H.; Starnawska, A.; Nyegaard, M.; Nielsen, A.L.; Børglum, A.; Mors, O. Saliva as a Blood Alternative for Genome-Wide DNA Methylation Profiling by Methylated DNA Immunoprecipitation (MeDIP) Sequencing. Epigenomes 2017, 1, 14. https://doi.org/10.3390/epigenomes1030014

AMA Style

Staunstrup NH, Starnawska A, Nyegaard M, Nielsen AL, Børglum A, Mors O. Saliva as a Blood Alternative for Genome-Wide DNA Methylation Profiling by Methylated DNA Immunoprecipitation (MeDIP) Sequencing. Epigenomes. 2017; 1(3):14. https://doi.org/10.3390/epigenomes1030014

Chicago/Turabian Style

Staunstrup, Nicklas H., Anna Starnawska, Mette Nyegaard, Anders L. Nielsen, Anders Børglum, and Ole Mors. 2017. "Saliva as a Blood Alternative for Genome-Wide DNA Methylation Profiling by Methylated DNA Immunoprecipitation (MeDIP) Sequencing" Epigenomes 1, no. 3: 14. https://doi.org/10.3390/epigenomes1030014

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Article Access Map by Country/Region

1
Back to TopTop