Next Article in Journal
Identification and Characterization of Nasal Polyposis and Mycoplasma Superinfection by Scanning Electron Microscopy and Nasal Cytology with Optical Microscopy: A Case Report
Previous Article in Journal
Acute Bronchiolitis: Is There a Role for Lung Ultrasound?
Open AccessArticle

mRNA Detection in Anal Cytology: A Feasible Approach for Anal Cancer Screening in Men Who Have Sex with Men Living With HIV

1
Institute Clinic of Gynaecology, Obstetrics, and Neonatology, Hospital Clínic—Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), University of Barcelona, 08036 Barcelona, Spain
2
Cepheid AB, 71 54 Solna, Sweden
3
Department of Pathology, Hospital Clínic, University of Barcelona, 08036 Barcelona, Spain
4
Department of Dermatology, Hospital Clinic of Barcelona, University of Barcelona, 08036 Barcelona, Spain
5
Cepheid AB, Sunnyvale, CA 94089, USA
6
Institut de Salut Global de Barcelona (ISGlobal), 08036 Barcelona, Spain
*
Author to whom correspondence should be addressed.
Diagnostics 2019, 9(4), 173; https://doi.org/10.3390/diagnostics9040173
Received: 29 June 2019 / Revised: 13 September 2019 / Accepted: 23 September 2019 / Published: 2 November 2019
(This article belongs to the Section Pathology and Molecular Diagnostics)
There is growing interest in anal cancer screening strategies. However, cytological/molecular evaluation of anal samples is challenging. We aimed to determine the feasibility of detecting, in anal liquid-based cytologies, the expression of biomarkers involved in the cell cycle disturbance elicited by human papillomavirus (HPV). The accuracy of this approach in the identification of high-grade squamous intraepithelial lesions/anal intraepithelial neoplasia grade2–3 (HSIL/AIN2–3) was also evaluated. 215 anal cytologies from men having sex with men living with human immunodeficiency virus were evaluated. Patients showing concordant cytological and anoscopy-directed biopsy diagnosis were selected: 70 with negative cytology and HPV test, 70 with low-grade SIL (LSIL/AIN1) cytology and biopsy, and 75 with cytology and biopsy of HSIL/AIN2–3. CDKN2A/p16, MKI67 and TOP2A mRNA expression was analyzed. HPV detection was performed with Xpert HPV Assay (Cepheid, Sunnyvale, CA, USA). HSIL/AIN2–3 showed higher expression for the biomarkers than LSIL/AIN1 or negative samples. The specificity for HSIL/AIN2–3 detection for a sensitivity established at 70% was 44.7% (95%confidence interval [CI] 36.5–53.2) for TOP2A and MKI67 and 54.5% (95%CI 46.0–62.8%) for CDKN2A/p16. mRNA detection of cell biomarkers in anal liquid-based cytology is feasible. Further studies are warranted to confirm if strategies based on mRNA detection have any role in anal cancer screening.
Keywords: anal cancer; anal cytology; HPV; mRNA; biomarkers anal cancer; anal cytology; HPV; mRNA; biomarkers
MDPI and ACS Style

Del Pino, M.; Martí, C.; Gaber, J.; Svanholm-Barrie, C.; Rodríguez-Carunchio, L.; Rodriguez-Trujillo, A.; Carreras, N.; Fuertes, I.; Barnadas, E.; Marimón, L.; Blanco, J.L.; Persing, D.H.; Torné, A.; Ordi, J. mRNA Detection in Anal Cytology: A Feasible Approach for Anal Cancer Screening in Men Who Have Sex with Men Living With HIV. Diagnostics 2019, 9, 173.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop