Next Article in Journal
Epidemiological Study of Autoimmune Bullous Dermatoses in Northeastern Romania
Next Article in Special Issue
Comparison of SD Bioline Malaria Ag Pf/Pan and Acro Malaria P.f./P.v./Pan with Microscopy and Real Time PCR for the Diagnosis of Human Plasmodium Species
Previous Article in Journal
Increased Risk of Benign Prostate Hyperplasia (BPH) in Patients with Gout: A Longitudinal Follow-Up Study Using a National Health Screening Cohort
Previous Article in Special Issue
The Significance of FilmArray Blood Culture Identification Panel (FA-BCID) for Managing Patients with Positive Blood Cultures
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Diagnostics
Volume 14
Issue 1
10.3390/diagnostics14010056
diagnostics-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

The Rapid Phenotypic Susceptibility Testing in Real-Life Experience: How the MIC Values Impact on Sepsis Fast Diagnostic Workflow

by
Giuseppe Migliorisi
1,*,
Maddalena Calvo
1,
Antonina Collura
2,
Francesca Di Bernardo
2,
Marianna Perez
3,
Guido Scalia
1,3 and
Stefania Stefani
1,3
1
U.O.C. Laboratory Analysis A.O.U. “Policlinico—San Marco”, Via Santa Sofia 78, 95123 Catania, Italy
2
U.O.C. Clinical Microbiology, “Civico-Di Cristina-Benfratelli” Hospital, Piazza Nicola Leotta 4, 90127 Palermo, Italy
3
Department of Biomedical and Biotechnological Sciences, University of Catania, Via Santa Sofia 97, 95123 Catania, Italy
*
Author to whom correspondence should be addressed.
Diagnostics 2024, 14(1), 56; https://doi.org/10.3390/diagnostics14010056
Submission received: 18 November 2023 / Revised: 22 December 2023 / Accepted: 25 December 2023 / Published: 26 December 2023
(This article belongs to the Special Issue Laboratory Diagnosis in Microbial Diseases, 2nd Edition)
Browse Figures
Versions Notes

Abstract

The MIC value definition faithfully reflects antimicrobial sensitivity, profoundly impacting the infection's clinical outcome. Our study aimed to evaluate the Accelerate PhenoTM System in defining the importance of fast phenotypic susceptibility data. A number of 270 monomicrobial samples simultaneously underwent standard procedures and fast protocols after a contemporary Gram stain. Finally, we provided Turn-around Time (TAT) and statistical evaluations. The fast technology required a medium value of 7 h to complete ID and AST profiles. Although there were some spectrum limitations, it revealed an optimal success rate in microbial identification directly from positive blood cultures. The Gram-negative AST reached a 98.9% agreement between the Accelerate Pheno™ System and the standard method. In addition, the Gram-positive AST gathered a 98.7% agreement comparing the same systems. The chance to rapidly provide precise MIC values is one of the last frontiers in clinical microbiology, especially in high-prevalence antimicrobial resistance areas.
Keywords: sepsis; MIC value; rapid phenotypic AST sepsis; MIC value; rapid phenotypic AST

Share and Cite

MDPI and ACS Style

Migliorisi, G.; Calvo, M.; Collura, A.; Di Bernardo, F.; Perez, M.; Scalia, G.; Stefani, S. The Rapid Phenotypic Susceptibility Testing in Real-Life Experience: How the MIC Values Impact on Sepsis Fast Diagnostic Workflow. Diagnostics 2024, 14, 56. https://doi.org/10.3390/diagnostics14010056

AMA Style

Migliorisi G, Calvo M, Collura A, Di Bernardo F, Perez M, Scalia G, Stefani S. The Rapid Phenotypic Susceptibility Testing in Real-Life Experience: How the MIC Values Impact on Sepsis Fast Diagnostic Workflow. Diagnostics. 2024; 14(1):56. https://doi.org/10.3390/diagnostics14010056

Chicago/Turabian Style

Migliorisi, Giuseppe, Maddalena Calvo, Antonina Collura, Francesca Di Bernardo, Marianna Perez, Guido Scalia, and Stefania Stefani. 2024. "The Rapid Phenotypic Susceptibility Testing in Real-Life Experience: How the MIC Values Impact on Sepsis Fast Diagnostic Workflow" Diagnostics 14, no. 1: 56. https://doi.org/10.3390/diagnostics14010056

APA Style

Migliorisi, G., Calvo, M., Collura, A., Di Bernardo, F., Perez, M., Scalia, G., & Stefani, S. (2024). The Rapid Phenotypic Susceptibility Testing in Real-Life Experience: How the MIC Values Impact on Sepsis Fast Diagnostic Workflow. Diagnostics, 14(1), 56. https://doi.org/10.3390/diagnostics14010056

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop