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Open AccessArticle

Comparison of Ki-67 Labeling Index Patterns of Diffuse Large B-Cell Lymphomas and Burkitt Lymphomas Using Image Analysis: A Multicenter Study

1
Department of Hospital Pathology, Yeouido St. Mary’s Hospital, The Catholic University of Korea College of Medicine, Seoul 07345, Korea
2
Department of Hospital Pathology, Uijeongbu St. Mary’s Hospital, The Catholic University of Korea College of Medicine, Uijeongbu 11765, Korea
3
Department of Hospital Pathology, Incheon St. Mary’s Hospital, The Catholic University of Korea College of Medicine, Incheon 21431, Korea
4
Department of Hospital Pathology, St. Vincent’s Hospital, The Catholic University of Korea College of Medicine, Suwon 16247, Korea
5
Department of Hospital Pathology, Bucheon St. Mary’s Hospital, The Catholic University of Korea College of Medicine, Bucheon 14647, Korea
6
Department of Hospital Pathology, Daejeon St. Mary’s Hospital, The Catholic University of Korea College of Medicine, Daejeon 35301, Korea
7
Department of Hospital Pathology, Eunpyeong St. Mary’s Hospital, The Catholic University of Korea College of Medicine, Seoul 03312, Korea
8
Department of Hospital Pathology, Seoul St. Mary’s Hospital, The Catholic University of Korea College of Medicine, Seoul 06591, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Ludmilla Morozova-Roche
Diagnostics 2021, 11(2), 343; https://doi.org/10.3390/diagnostics11020343
Received: 22 January 2021 / Revised: 17 February 2021 / Accepted: 17 February 2021 / Published: 19 February 2021
(This article belongs to the Collection Biomarkers in Medicine)
Diffuse large B-cell lymphoma (DLBCL) is the most common high-grade B-cell lymphoma found in Korea; it manifests with a variety of cellular morphologies and a high proliferation index. It is difficult to differentiate between DLBCL and Burkitt lymphoma (BL) based on immunohistochemistry, histology, and Epstein-Barr virus infection status owing to the overlap in findings. In this study, we performed comparative morphometric analysis to understand the proportional difference in Ki-67 staining between DLBCL and BL. We analyzed Ki-67-stained slides of 103 DLBCLs and 29 BLs that were pathologically confirmed using a three-tier classification system (negative, 1+, 2+, and 3+) to compare Ki-67 expression between BL and activated B-cell and germinal center B-cell subtypes of DLBCL and DLBCL with high proliferation indices (>90% of 2+ and 3+ cells). Patients with DLBCL were older than those with BL (62.1 versus 51.0 years). The number and proportion of negative cells (passenger and true negative cells) were significantly lower in BLs than those in DLBCLs (337.4, 5.9% versus 690.3, 12.4%). The number and proportion of 3+ cells were significantly higher in BLs than those in DLBCLs (5213.6, 96.3% versus 3132.4, 62.0%). BLs and DLBCLs with a high proliferation index showed similar results as those between BLs and overall DLBCLs. We were able to differentiate BLs and DLBCLs with 98.1% sensitivity and 100.0% specificity using an optimal cut-off of 97.9% of 2+/3+ Ki-67-positive cells. Thus, the Ki-67 labeling index may be a good differential biomarker for DLBCLs and BLs. View Full-Text
Keywords: diffuse large B-cell lymphoma; Burkitt lymphoma; Ki-67 antigen; computer-assisted image processing; differential diagnosis; image cytometry diffuse large B-cell lymphoma; Burkitt lymphoma; Ki-67 antigen; computer-assisted image processing; differential diagnosis; image cytometry
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MDPI and ACS Style

Chong, Y.; Kim, T.E.; Cho, U.; Jin, M.-S.; Yim, K.; Thakur, N.; Kim, J.O.; Cho, I.; Park, G. Comparison of Ki-67 Labeling Index Patterns of Diffuse Large B-Cell Lymphomas and Burkitt Lymphomas Using Image Analysis: A Multicenter Study. Diagnostics 2021, 11, 343. https://doi.org/10.3390/diagnostics11020343

AMA Style

Chong Y, Kim TE, Cho U, Jin M-S, Yim K, Thakur N, Kim JO, Cho I, Park G. Comparison of Ki-67 Labeling Index Patterns of Diffuse Large B-Cell Lymphomas and Burkitt Lymphomas Using Image Analysis: A Multicenter Study. Diagnostics. 2021; 11(2):343. https://doi.org/10.3390/diagnostics11020343

Chicago/Turabian Style

Chong, Yosep; Kim, Tae E.; Cho, Uiju; Jin, Min-Sun; Yim, Kwangil; Thakur, Nishant; Kim, Jong O.; Cho, Inju; Park, Gyeongsin. 2021. "Comparison of Ki-67 Labeling Index Patterns of Diffuse Large B-Cell Lymphomas and Burkitt Lymphomas Using Image Analysis: A Multicenter Study" Diagnostics 11, no. 2: 343. https://doi.org/10.3390/diagnostics11020343

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