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Open AccessArticle

Development of a Nucleic Acid Lateral Flow Immunoassay for the Detection of Human Polyomavirus BK

1
Biomedical Engineering Program, Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei 10607, Taiwan
2
Graduate Institute of Life Sciences, National Defense Medical Center, Taipei 11490, Taiwan
3
Department of Physiology and Biophysics, Graduate Institute of Physiology, National Defense Medical Center, Taipei 11490, Taiwan
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Institute of Preventive Medicine, National Defense Medical Center, Taipei 11490, Taiwan
5
Department and Graduate Institute of Biology and Anatomy, National Defense Medical Center, Taipei 11490, Taiwan
6
Department of Gerontological Health Care, National Taipei University of Nursing and Health Sciences, Taipei 11219, Taiwan
*
Authors to whom correspondence should be addressed.
Diagnostics 2020, 10(6), 403; https://doi.org/10.3390/diagnostics10060403
Received: 15 May 2020 / Revised: 5 June 2020 / Accepted: 10 June 2020 / Published: 12 June 2020
The BK virus (BKV) is an emerging pathogen in immunocompromised individuals and widespread in the human population. Polymerase chain reaction is a simple and highly sensitive method for detecting BKV, but it is time consuming and requires expensive instruments and expert judgment. The lateral flow assay, a rapid, low-cost, minimal-labor, and easy-to-use diagnostic method, was successfully applied for pathogen detection. In this study, we used oligonucleotide probes to develop a simple and rapid sandwich-type lateral flow immunoassay for detecting BKV DNA within 45 minutes. The detection limit for the synthetic single-stranded DNA was 5 nM. The specificity study showed no cross-reactivity with other polyomaviruses, such as JC virus and simian virus 40. For the Escherichia coli containing BKV plasmid cultured samples, the sensitivity was determined to be 107 copies/mL. The approach offers great potential for BKV detection of various target analytes in point-of-care settings. View Full-Text
Keywords: BK polyomavirus; lateral flow immunoassay; nucleic acid testing; point-of-care testing BK polyomavirus; lateral flow immunoassay; nucleic acid testing; point-of-care testing
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Huang, Y.-H.; Yu, K.-Y.; Huang, S.-P.; Chuang, H.-W.; Lin, W.-Z.; Cherng, J.-H.; Hung, Y.-W.; Yeh, M.-K.; Hong, P.-D.; Liu, C.-C. Development of a Nucleic Acid Lateral Flow Immunoassay for the Detection of Human Polyomavirus BK. Diagnostics 2020, 10, 403.

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